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Plasmid#114773PurposePrey vector NIK2 A9_pECIA14 should be used with bait vector NIK2 A9_pECIA2.DepositorInsertAT3G25560
UseTagsExpressionInsectMutationPromoterMetallothionein (Copper-Inducible)Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
SARK A10_pECIA14
Plasmid#114774PurposePrey vector SARK A10_pECIA14 should be used with bait vector SARK A10_pECIA2.DepositorInsertAT4G30520
UseTagsExpressionInsectMutationPromoterMetallothionein (Copper-Inducible)Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
IOS1 A1_pECIA14
Plasmid#114736PurposePrey vector IOS1 A1_pECIA14 should be used with bait vector IOS1 A1_pECIA2.DepositorInsertAT1G51800
UseTagsExpressionInsectMutationPromoterMetallothionein (Copper-Inducible)Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-FLEX-macaque OPN4-IRES-EGFP
Plasmid#223253PurposeAAV construct for Cre-dependent (FLEX) expression of macaque melanopsin (Opn4, short isoform, GenBank:OP856641; hSyn promoter) and of EGFP via an IRES.DepositorInsertmacaque OPN4-IRES-EGFP (OPN4 )
UseAAVTagsExpressionMutationPromoterhuman Synapsin 1 (hSyn)Available sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPK-351
Plasmid#157921PurposepcDNA-CMV-PIF3MTAD-IRES-PhyBGal4DBDDepositorInsertsPIF3-MTAD
IRES
PhyB(1-621)-SV40NLS-Gal4DBD
UseSynthetic Biology; OptogeneticsTagsHA tag and SV40NLSExpressionMammalianMutationpif3 1-523PromoterCMVAvailable sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
CIBN-CAAX
Plasmid#79574Purposeexpression of N-terminal portion of CIB1 with a C-terminal CAAX box from KRras for plasma-membrane targetingDepositorInsertCIBN (CIB1 Mustard Weed)
UseTagsC-terminal CAAX-boxExpressionMammalianMutationK93A, R94A, K106A, K107A (please see depositor co…PromoterCMVAvailable sinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2-CRY2-mCherry
Plasmid#140571PurposeFor use in light-induced protein inactivation through the interaction with CIB1DepositorInsertCRY2 (CRY2 Mustard Weed)
UseTagsmCherryExpressionMammalianMutationPromoterCMV IE94Available sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pAAV hSyn1 CaRhAC T2A tDimer
Plasmid#101722Purpose- humanized - variant of pAAV hSyn YFP-CaRhAC Addgene # 101721– less reliable in hippocampal neuronsDepositorInsertsCatRhAC
red fluorescent protein
UseAAVTagsExpressionMutationE497K,C569DPromoterhuman Synapsin1 promotor and ribosomal skip seque…Available sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
Ef1a-DIO-BiPOLES-mCerulean
Plasmid#154949PurposeOptogenetic tool for blue-light inhibition and red-light excitation of neuronsDepositorInsertBiPOLES
UseAAVTagsExpressionMammalianMutationPromoterEf1-alphaAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
mDlx-BiPOLES-mCerulean
Plasmid#154946PurposeOptogenetic tool for blue-light inhibition and red-light excitation of neuronsDepositorInsertBiPOLES
UseAAVTagsExpressionMammalianMutationPromoterminimal DlxAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn1 CaRhGC wt T2A tDimer
Plasmid#101720Purposehumanized, Neuron-specific promoter driven expression of the rhodopsin guanylyl cyclase from Catenaria anguillulae with a neuron-specific promoter. Useful for raising intracellular cAMP close to the mDepositorInsertsCatRhGC
red fluorescent protein
UseAAVTagsExpressionMammalianMutationPromoterhSyn1 and ribosomal skip sequence T2AAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
NLS-tdPCP-CIBN
Plasmid#183937PurposePCP tandem dimer binding to PP7 RNA stem loops coupled to optogenetic CIBN domain; bound by PHR upon blue light exposureDepositorInserttdPCP-CIBN (CIB1 Mustard Weed, Synthetic)
UseTagsNLSExpressionMammalianMutationnonePromoterCMV (TATA box removed)Available sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPKm-230
Plasmid#90499PurposepSIN - EF-1alpha - PIF3 - MTAD - IRES - PhyB - GBD, dual vector of PIF3-MTAD under EF-1 alpha promoter and PhyB-DBD under IRES promoter; See growth conditions below.DepositorInsertmito-tFD and mito-tFNR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
PHR-GFP-VP16
Plasmid#183927PurposeOptogenetic PHR domain coupled to GFP and VP16 activation domain; binds to CIBN upon blue light exposureDepositorUseTagsExpressionMammalianMutationnonePromoterCMVAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
PHR-GFP-FUSN-VP16
Plasmid#183933PurposeOptogenetic PHR domain coupled to GFP, FUSN (high phase separation propensity) and VP16 activation domain; binds to CIBN upon blue light exposureDepositorUseTagsExpressionMammalianMutationnonePromoterCMVAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
DRH337: AAV-hsyn-CheRiff-eGFP
Plasmid#51697PurposeAAV production of CheRiff channelrhodopsin actuatorDepositorInsertCheRiff-eGFP
UseAAVTagseGFPExpressionMutationPromoterhSynapsinAvailable sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
CaMKII-somBiPOLES-mCerulean
Plasmid#154948PurposeOptogenetic tool for blue-light inhibition and red-light excitation of neuronsDepositorHas ServiceAAV Retrograde, AAV5, and AAV9InsertsomBiPOLES
UseAAVTagssoma-targeting motif from Kv2.1 channelExpressionMammalianMutationPromoterCaMKIIa(0.4)Available sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE503
Plasmid#153276PurposeIntermediate sgRNA array cloning vector position 1DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE504
Plasmid#153277PurposeIntermediate sgRNA array cloning vector position 2DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only