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  1. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...s41565-023-01419-x Deverman, B. E., Pravdo, P. L., Simpson, B. P., Kumar, S. R., Chan, K. Y., Banerjee, A...Hordeaux, J., Yuan, Y., Clark, P. M., Wang, Q., Martino, R. A., Sims, J. J., Bell, P., Raymond, A., Stanford,..., M., & Sabeti, P. C. (2023). Systemic administration of novel engineered AAV capsids facilitates enhanced...Wagers, A. J., & Sabeti, P. C. (2021). Directed evolution of a family of AAV capsid variants enabling potent... A. I., Dvoryanchikov, G., Makhoul, V., Tsoulfas, P., & Chaudhari, N. (2022). Selectively Imaging Cranial...Griffiths, J. A., Ichiki, T., Mazmanian, S. K., Osborne, P. B., Keast, J. R., Miller, C. T., Fox, A. S., Chiu... N. M., Sun, J., Chen, X., Lefevre, A., Singh, V. P., Arokiaraj, C. M., Shay, T. F., Vendemiatti, J., ...

  2. Tips for arabidopsis transformation

    Type
    Blog Post
    ...transformation’, Plant Methods. BioMed Central, 2(1), p. 19. doi: 10.1186/1746-4811-2-19. PubMed PMID: 17087829...CRISPR/Cas9 Using pSiM24 for simplifying plant genetic engineering Using arabidopsis in the classroom ..., a graduate student at Stanford University. Arabidopsis is a fantastic model organism for many reasons... are many motivations to generate transgenic Arabidopsis, from studying transcriptional and translational... plants, to complementing mutant phenotypes. Arabidopsis is amenable to the floral drip or dip transformation...transformation protocol, as well as protocols for growing Arabidopsis are also available (Weigel and Glazebrook, 2002...vector is the first step in generating transgenic Arabidopsis. There are a bunch of great options, but here...

  3. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...Many thanks to our guest blogger Derek Simon! Derek P. Simon received his PhD in Cellular and Molecular ...number of the opsin genes to alter the light spectrum for activation and the kinetics of opsin activity/inactivity...viruses (not expressing the opsin) are also available. Picking your opsin  You must consider whether you...  In brief, some standard opsins (see table above) include Channelrhodopsin2 (Chr2), a blue-light sensitive...silence neurons than HR. Delivering your opsin  The standard opsin delivery method is local injection of ...expression of your opsin is no longer required to achieve cell-type specific opsin expression. However...stimulation. Testing opsin expression  Regardless of the method of expression of your opsin gene, it is essential...

  4. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...transfer with a psi membrane stacked behind the p membrane. If your protein runs through the p membrane into... try using a 0.2 µm (psi) membrane for small proteins (< 15 kDa) and a 0.45 µm (p) membrane for larger...into the psi membrane, that’s a good indication that the p membrane pores are too large.  Transfer method...-transferring Transfer with two membranes* Psi membrane Semi-dry transfer Decrease voltage...

  5. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...transgene expression. Psi (Ψ) RNA target site for packaging by Nucleocapsid. WPRE Woodchuck hepatitis... resources References Swift S, Lorens J, Achacoso P, Nolan GP. Rapid production of retroviruses for efficient...retroviral particle containing Matrix, Capsid, and Nucleocapsid components. Pol Precursor protein...

  6. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ... C, Deveau H, Richards M, Boyaval P, Moineau S, Romero DA, Horvath P. 2007. CRISPR provides acquired resistance... M, Loehr J, Jacquet K, Huard CC, Roques C, Herst P, Côté J, Doyon Y. 2015. A Scalable Genome-Editing-...4. PMID: 27096365 Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T. 2015. Multicolor CRISPR...Barrangou R, Brouns SJ, Charpentier E, Haft DH, Horvath P, Moineau S, Mojica FJ, Terns RM, Terns MP, White MF...Rev Microbiol . 13(11):722-36. PMID: 26411297 Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville...Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin...PMID: 26422227 Zetsche B, Heidenreich M, Mohanraju P, Fedorova I, Kneppers J, DeGennaro EM, Winblad N, ...

  7. Deisseroth INTRSECT Collection

    Type
    Collection
    ...PubMed (Link opens in a new window) Tovote P, Esposito MS, Botta P, Chaudun F, Fadok JP, Markovic M, Wolff... Deisseroth K, Zhao F, Luo MH, Gong L, He M, Zhou P, Paninski L, Li B. 2017. The central amygdala controls...Venkataraju K, Straub C, Wang W, Robertson K, Osten P, Sabatini BL. 2019. Distinct Cortical-Thalamic-Striatal...additional fluorophores, excitatory and inhibitory opsins , genetically-encoded calcium indicators, and rabies... Cre Yes Dual recombinase-dependent: Excitatory Opsins Addgene ID Plasmid Logic Sites and Mutations In-Stock... Cre Yes Dual recombinase-dependent: Inhibitory Opsins Addgene ID Plasmid Logic Sites and Mutations In-Stock...

  8. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...Sands, J., Sahasrabudhe, A., Anikeeva, P., & Chandrakasan, A. P. (2020). Modular Optoelectronic System...EMBC44109.2020.9175600 Park, S., Loke, G., Fink, Y., & Anikeeva, P. (2019). Flexible fiber-based optoelectronics for ...Niroui, F., Fink, Y., Chandrakasan, A., … Anikeeva, P. (2023). Multifunctional microelectronic fibers enable... by delivering an AAV construct expressing channelrhodopsin 2 (ChR2), a blue light sensitive cation channel...This measurement trended with expression of the opsin in the target neurons, validating the functionality... Plasmid tools for microbiome studies Channel rhodopsins for optogenetics Viral Vectors 101: Chemogenetics...

  9. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...Adeishvili, N., Berthold, P., Ollig, D., Hegemann, P., & Bamberg, E. (2003). Channelrhodopsin-2, a directly light-gated...Papagiakoumou, E., Gottschalk, A., Soba, P., Emiliani, V., Engel, A. K., Hegemann, P., & Wiegert, J. S. (2021). BiPOLES...Berndt, A., Sohal, V. S., Deisseroth, K., & Hegemann, P. (2010). Ultrafast optogenetic control. Nature Neuroscience....1936192100 Tichy, A.-M., Gerrard, E. J., Sexton, P. M., & Janovjak, H. (2019). Light-activated chimeric...light-activated tool for controlling neuronal activity, channelrhodopsin-2 (ChR2), a blue light-activated cation channel...Light-gated ion channels Light activated ion channels (rhodopsins) either excite or inhibit neuronal activity upon... depolarize it (Nagel et al., 2003). Inhibitory opsins are usually light-driven pumps that pass ions (...

  10. Luciferase Plasmid Collection

    Type
    Collection
    ...Gateway cloning. Use with 174050 Alberto Macho 136010 psi-CHECK3 Firefly/Renilla Insertion of 3' UTR sequences...HSV TK promoter for normalization. Modified from psi-CHECK2 so both Renilla and Firefly luciferase contain...expression of firefly luciferase Ming-Chih Lai 115366 p CIneo-RL Renilla CMV Mammalian expression of humanized...

  11. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...y Hanlon, K. S., Kleinstiver, B. P., Garcia, S. P., Zaborowski, M. P., Volak, A., Spirig, S. E., Muller... C., Ingelsson, M., Chamberlain, J. S., Corey, D. P., Aryee, M. J., Joung, J. K., Breakefield, X. O., ...be packaged in a variety of capsids. To date, at least 12 different capsids, or serotypes, have been identified... AAV, with the genome from one serotype and the capsid of another. Researchers have used these psuedotypes...

  12. Fujii Lab CRISPR Plasmids

    Type
    Collection
    ...with the neomycin resistance gene 64325 3xFLAG-dCas9/p-bacteria Expresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent...M, von Stedingk K, Fujita T, Persson CU, Eriksson P, Veerla S, De Preter K, Speleman F, Fujii H, Påhlman...target human IRF-1 promoter 61080 gRNA-hIRF-1 #12/pSIR Expresses a guide RNA (gRNA) to target human IRF...

  13. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...Chadeuf G, Ciron C, Moullier P, Salvetti A (2005) Evidence for Encapsidation of Prokaryotic Sequences during...Lecomte E, Tournaire B, Cogné B, Dupont J-B, Lindenbaum P, Martin-Fontaine M, Broucque F, Robin C, Hebben M,...Merten O-W, Blouin V, François A, Redon R, Moullier P, Léger A (2015) Advanced Characterization of DNA Molecules...small predetermined seed sequences unique to the capsid plasmid. Our recombination software looks for and...

  14. An Introduction to Adenovirus

    Type
    Blog Post
    ...10.1016/B978-0-12-801238-3.02523-X. Hofmann, C., Löser, P., Cichon, G., Arnold, W., Both, G. W., & Strauss, ...doi.org/10.1136/jcp.s3-6.1.39. PMID: 4376152. Rowe, W. P., Huebner, R. J., Gilmore, L. K., Parrott, R. H., ...R. L., Garcia, K. Y., Goodrich, J. S., Lovett, S. P., Parker, K., Radune, D., Hatada, A., Pan, C. Y., ...Rizzo, K., Bertumen, J. B., Morales, C., Oluniyi, P. E., Nguyen, J., … Chiu, C. Y. (2023). Adeno-associated... (and their structural proteins, which would encapsidate new virions) are not expressed. Note that replication-competent...

  15. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...scale.     References Bezanilla M, Perroud P ‐F., Pan A, Klueh P, Quatrano RS (2005) An RNAi System in Physcomitrella...Clauder-Münster S, Eberhard D, Fälth Savitski M, Grandi P, Jakob P, Michon A-M, Sun H, Tessmer K, Bürckstümmer T...amiRNAs based upon miRNA precursors of rice and Arabidopsis species, respectively. Short hairpin RNA (shRNA...plants, such as in the model flowering plant Arabidopsis thaliana (Li et al., 2013; Zhang et al., 2018...

  16. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    ...stays open for a longer duration upon activation. p-mcherry-C1-iC++_CA - A slow cycling (open for minutes...20026026. PubMed Central PMCID: PMC2823934. Pai, Vaibhav P., et al. "Transmembrane voltage potential controls...specificity. Stable Step Function Opsin - A stabilized step function opsin (SSFO) for optogenetic activation...metagenomically discovered marine anion-conducting channelrhodopsin used as a sensitive fluorescent voltage indicator...Hyperpolarizing chloride channel SwiChr++ - A channelrhodopsin converted into a chloride-conducting anion... step-function artificial anion conducting channelrhodopsin (aACR). It is activated by cyan light and ...

  17. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...References: Allocca M, Doria M, Petrillo M, Colella P, Garcia-Hoyos M, Gibbs D, Kim SR, Maguire A, Rex TS...https://doi.org/10.1038/nbt1153 Odom GL, Gregorevic P, Allen JM, Chamberlain JS (2011) Gene Therapy of mdx...://doi.org/10.1038/mt.2010.205 Trapani I, Colella P, Sommella A, Iodice C, Cesi G, Simone S, Marrocco ....201302948 Trapani I, Toriello E, de Simone S, Colella P, Iodice C, Polishchuk EV, Sommella A, Colecchi L, ...the Packaging Capacity of Adeno-Associated Virus Capsids. Human Gene Therapy Methods 27:1–12 . https://doi.org...

  18. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...Wagers, A. J., & Sabeti, P. C. (2021). Directed evolution of a family of AAV capsid variants enabling potent..., Papworth, G., Watkins, S., Trucco, M., Robbins, P. D., Li, J., & Xiao, X. (2006). Widespread and Stable... Emily P. Bentley...vectors have both distinctive capsids and genomic structures. The viral capsid is the key that lets AAVs ...of promoter/enhancer. Table 1: AAV capsids and receptors. Capsid Primary receptor Coreceptor AAV1... true! In a previous post, we covered systemic capsids, which allow AAVs to broadly spread throughout ...particular cell type depends largely on the viral capsid, but specificity doesn’t end there: expression ...

  19. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...PubMed Central PMCID: PMC2593125. 2. Buchholz, F, P O Angrand, and A. Francis Stewart. 1998. “Improved...Central PMCID: PMC3655711. 4. Gronostajski, R M, and P D Sadowski. 1985. “The FLP Recombinase of the Saccharomyces...PMC41617. 7. Metzger, D, J Clifford, H Chiba, and P Chambon. 1995. “Conditional Site-Specific Recombination... M. Sternson. 2008. “A FLEX Switch Targets Channelrhodopsin-2 to Multiple Cell Types for Imaging and Long-Range...
Showing: 1 - 20 of 61 results