We narrowed to 2,065 results for: gibson assembly

1. pST39-Mega'-deltaSJ

   Plasmid

   #216936

   Purpose

   Mega' with knockout of GvpS and GvpJ

   Depositor

   George Lu

   Article

   Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

   Insert

   Mega' with knockout of GvpS and GvpJ
   

   Expression

   Bacterial

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pST39-Mega'-deltaG

   Plasmid

   #216883

   Purpose

   Mega' with knockout of GvpG

   Depositor

   George Lu

   Article

   Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

   Insert

   Mega' with knockout of GvpG
   

   Expression

   Bacterial

   Available Since

   Sept. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pST39-Mega'-deltaLJ

   Plasmid

   #216932

   Purpose

   Mega' with knockout of GvpL and GvpJ

   Depositor

   George Lu

   Article

   Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

   Insert

   Mega' with knockout of GvpL and GvpJ
   

   Expression

   Bacterial

   Available Since

   Sept. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pST39-Mega'-deltaLK

   Plasmid

   #216931

   Purpose

   Mega' with knockout of GvpL and GvpK

   Depositor

   George Lu

   Article

   Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

   Insert

   Mega' with knockout of GvpL and GvpK
   

   Expression

   Bacterial

   Available Since

   Sept. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pST39-Mega'-deltaGU

   Plasmid

   #216929

   Purpose

   Mega' with knockout of GvpG and GvpU

   Depositor

   George Lu

   Article

   Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

   Insert

   Mega' with knockout of GvpG and GvpU
   

   Expression

   Bacterial

   Available Since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pST39-Mega'-deltaGT

   Plasmid

   #216928

   Purpose

   Mega' with knockout of GvpG and GvpT

   Depositor

   George Lu

   Article

   Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

   Insert

   Mega' with knockout of GvpG and GvpT
   

   Expression

   Bacterial

   Available Since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pST39-Mega'-deltaGK

   Plasmid

   #216926

   Purpose

   Mega' with knockout of GvpG and GvpK

   Depositor

   George Lu

   Article

   Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

   Insert

   Mega' with knockout of GvpG and GvpK
   

   Expression

   Bacterial

   Available Since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pST39-Mega'-deltaGJ

   Plasmid

   #216927

   Purpose

   Mega' with knockout of GvpG and GvpJ

   Depositor

   George Lu

   Article

   Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

   Insert

   Mega' with knockout of GvpG and GvpJ
   

   Expression

   Bacterial

   Available Since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pST39-Mega'-deltaTU

   Plasmid

   #216944

   Purpose

   Mega' with knockout of GvpT and GvpU

   Depositor

   George Lu

   Article

   Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

   Insert

   Mega' with knockout of GvpT and GvpU
   

   Expression

   Bacterial

   Available Since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1-39S Light chain

    Plasmid

    #216297

    Purpose

    Expresses anti-HER2 39S Light chain in mammalian cells. To be paired with 39S heavy chain to form the anti-HER2 39S Fab

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    39S Light chain
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Signal peptide

    Expression

    Mammalian

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1-MF3958 Light chain

    Plasmid

    #216304

    Purpose

    Expresses anti-HER2 MF3958 Light chain in mammalian cells. To be paired with MF3958 heavy chain to form the anti-HER2 MF3958 Fab

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    MF3958 Light chain
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Signal peptide

    Expression

    Mammalian

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPW3757 : CMVd3-ERmembrane-2xHaloTag-KKMP

    Plasmid

    #185679

    Purpose

    Low-level transient expression of two tandem HaloTag proteins targeted to the ER membrane.

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERmembrane-2xHaloTag-KKMP
    

    Expression

    Mammalian

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFD-MBP-CS(TEV)-StrepTag-II-AI(TVMV)

    Plasmid

    #137110

    Purpose

    iFLinkC Linker cloning

    Depositor

    Viktor Stein

    Article

    Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

    Insert

    MBP-CS(TEV)-StrepTag-II-AI(TVMV)
    

    Use

    Synthetic Biology

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pQE80L TriCatcher-MMP

    Plasmid

    #112632

    Purpose

    Two SpyCatchers linked by an elastin-like polypeptide with an MMP-cleavable sequence and a C-terminal SnoopCatcher

    Depositor

    Mark Howarth

    Article

    Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

    Insert

    TriCatcher-MMP
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    central matrix metalloproteinase-cleavable sequen…

    Promoter

    T5

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p005-RFP-weak

    Plasmid

    #108316

    Purpose

    Synthetic RFP based on Corynactis spp under weak synthetic constitutive promoter pFAB4024; kanamycin resistant.

    Depositor

    Jaroslaw Bryk

    Article

    Siddall et al bioRxiv 2021.06.20.449138

    Insert

    RFP under controlf of weak synthetic constitutive promoter pFAB4024
    

    Expression

    Bacterial

    Promoter

    pFAB4024

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. psBIG1a

    Plasmid

    #229957

    Purpose

    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. psBIG1b

    Plasmid

    #229958

    Purpose

    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. psBIG2abc

    Plasmid

    #229963

    Purpose

    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. psBIG2ab

    Plasmid

    #229962

    Purpose

    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. psBIG2abcd

    Plasmid

    #229964

    Purpose

    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge