We narrowed to 16,259 results for: GRN

1. pNTI661 pRPR1(TetO)-sgRNA

   Plasmid

   #139475

   Purpose

   Vector for tet-regulated sgRNA expression in yeast

   Depositor

   Nicholas Ingolia

   Article

   McGlincy et al BMC Genomics. 2021 Mar 23;22(1):205. doi

   Insert

   single guide RNA scaffold
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   pRPR1(TetO)

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCR-U6-gRNA-miniCMV-TdTomato

   Plasmid

   #192656

   Purpose

   A plasmid encoding miniCMV sgRNA and miniCMV-promoter-TdTomato

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   TdTomato
   

   Expression

   Mammalian

   Promoter

   U6, miniCMV

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CMVp-dsRed2-Triplex-28-gRNA1-28-pA

   Plasmid

   #55200

   Purpose

   Plasmid encoding the Triplex/gRNA architecture.This is a modified form of the original plasmid described in the paper (Construct 3). mKate2 was replaced with dsRed2 because of distribution issues.

   Depositor

   Timothy Lu

   Article

   Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

   Insert

   dsRed2
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLKO.1-puro U6 sgRNA CAG

   Plasmid

   #50927

   Purpose

   U6 driven sgRNA negative control

   Depositor

   Rene Maehr, Scot Wolfe

   Article

   Kearns et al Development. 2014 Jan;141(1):219-23. doi

   Insert

   negative control sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pC0043-PspCas13b-gRNA LINE1

   Plasmid

   #223699

   Purpose

   gRNA of dPspCas13b-FTO targeting LINE1

   Depositor

   Chuan He

   Article

   Wei et al Science. 2022 May 27;376(6596):968-973.

   Insert

   gRNA target sequence for LINE1
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMD19-PBBa_J23117-sgRNA-Spr

   Plasmid

   #190793

   Purpose

   Template vector to amplify single sgRNAs or pieces for multiplexing arrays. Has flanking BsaI-sites.

   Depositor

   Paul Hudson

   Article

   Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

   Insert

   sgRNA (dummy)
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   BBa_J23117

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pKLV2-U6gRNA5(BbsI)-PGKpuro2AZsG-W

   Plasmid

   #67975

   Purpose

   CRISPR gRNA expression vector with an improved scaffold and puro/ZsG markers

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKpuro2AZsG cassette, WPRE
   

   Use

   Lentiviral

   Mutation

   Deleted BbsI site within WPRE

   Promoter

   human U6 promoter, mouse PGK promoter

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. px335-sgRNA-EF(Cas9n)-Dnmt3b-R

   Plasmid

   #122333

   Purpose

   Expresses sgRNA targeting mouse Dnmt3b and Cas9 nickase in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   sgRNA for mouse Dnmt3b
   

   Expression

   Mammalian

   Available Since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. px335-sgRNA-EF(Cas9n)-Dnmt3b-L

   Plasmid

   #122334

   Purpose

   Expresses sgRNA targeting mouse Dnmt3b and Cas9 nickase in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   sgRNA for mouse Dnmt3b
   

   Expression

   Mammalian

   Available Since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLH-sgRNA-Sirius-8XPP7

    Plasmid

    #121940

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    sgRNA-Sirius-8XPP7
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Promoter

    human U6 promoter

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDY0207 ACTB atgRNA with v1 scaffold

    Plasmid

    #179107

    Purpose

    ACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    ACTB N-term PBS 13 RT 29 AttB 46 atgRNA
    

    Use

    CRISPR

    Available Since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pU6-sp-sgRNA-mDNMT1_nick-lenti

    Plasmid

    #135959

    Purpose

    S. pyogenes nicking sgRNA for mouse DNMT1

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    mDNMT1_nick
    

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    U6

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUb-Cas9-mCherry_cU6:6-sgRNA

    Plasmid

    #190598

    Purpose

    The plasmid encodes S. pyogenes Cas9 and mCherry separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter

    Depositor

    Claudia Rueckert

    Article

    Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.

    Inserts

    Cas9
    sgRNA
    

    Use

    CRISPR

    Tags

    mCherry - separated by T2A

    Promoter

    Aedes aegypti polyubiquitin and Culex quinquefasc…

    Available Since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pX335-NQL002-WAPL-sgRNA1

    Plasmid

    #175550

    Purpose

    For transient expression of spCas9-nickase and one sgRNA targeting the mouse WAPL locus. Use together with pX335-NQL003-WAPL-sgRNA2 targeting construct.

    Depositor

    Elzo de Wit

    Article

    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

    Insert

    spCas9-nickase and sgRNA against mouse WAPL STOP Codon
    

    Use

    Mouse Targeting

    Expression

    Mammalian

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pX335-NQL003-WAPL-sgRNA2

    Plasmid

    #175551

    Purpose

    For transient expression of spCas9-nickase and one sgRNA targeting the mouse WAPL locus. Use together with pX335-NQL002-WAPL-sgRNA1 targeting construct.

    Depositor

    Elzo de Wit

    Article

    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

    Insert

    spCas9-nickase and sgRNA against mouse WAPL STOP Codon
    

    Use

    Mouse Targeting

    Expression

    Mammalian

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCR8-attL5-pegRNA-attR3

    Plasmid

    #213054

    Purpose

    Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attR3

    Depositor

    Bing Yang

    Article

    Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

    Insert

    sgRNA
    

    Expression

    Bacterial

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. hU6-pegRNA-EF-1α-Cre Template

    Plasmid

    #196036

    Purpose

    The template UPEC vector designed for cloning of a single pegRNA and subsequent co-expression with Cre.

    Depositor

    Tyler Jacks

    Article

    Ely et al Nat Biotechnol. 2023 May 11. doi: 10.103

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    hU6 and EF-1-alpha

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. gRNA3_NIPBL_Cas9-hGem-for_N-terminal_tagging

    Plasmid

    #217662

    Purpose

    expresses Cas9-hGem and guideRNA for N terminal tagging of NIPBL

    Depositor

    Daniel Gerlich

    Article

    Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

    Insert

    sgRNA3 for N terminal tagging of NIPBL (NIPBL Human)
    

    Use

    CRISPR

    Expression

    Bacterial and Mammalian

    Promoter

    U6

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CaTCH Dual-sgRNA_sgBC1-A_sgBC1-C

    Plasmid

    #154196

    Purpose

    Lentiviral expression plasmid encoding two sgRNAs for targeting of the CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker.

    Depositor

    Anna Obenauf

    Article

    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

    Insert

    sgRNA-BC1-A, sgRNA-BC1-C
    

    Use

    Lentiviral; Catch barcode activation

    Expression

    Mammalian

    Promoter

    hU6 and mU6

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKLV-U6gRNA-EF(BbsI)-PGKpuro2ABFP

    Plasmid

    #62348

    Purpose

    An empty optimized gRNA expression vector

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Nucleic Acids Res. 2015 Jun 19. pii: gkv

    Insert

    none
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge