We narrowed to 15,948 results for: GRN

1. CMVp-dsRed2-Triplex-28-gRNA3-28-gRNA4-28-gRNA5-28-gRNA6-28-pA

   Plasmid

   #55202

   Purpose

   Plasmid encoding multiplexed 4x gRNAs. This is a modified form of the original plasmid described in the paper (Construct 19). mKate2 was replaced with dsRed2 because of distribution issues.

   Depositor

   Timothy Lu

   Article

   Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

   Insert

   dsRed2
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pT2K-CAGGS-U6-sgRNA-M5-U6-sgRNA-M7-U6-sgRNA-M9-IRES-CFP

   Plasmid

   #114729

   Purpose

   3 sgRNAs targeting a sequence upstream of the initiator ATG of the cellular Myc gene

   Depositor

   Martine Roussel

   Article

   Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

   Insert

   sgRNA M5, sgRNA M7, sgRNA M9
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Construct 31 - 1xTetO_H1p_20nt2_stgRNA_3xLacO_U6p_20nt3_stgRNA

   Plasmid

   #81254

   Purpose

   For expressing Dox and IPTG inducible stgRNAs

   Depositor

   Timothy Lu

   Article

   Perli et al Science. 2016 Aug 18. pii: aag0511.

   Insert

   stgRNA
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSpCas9_gRNA1TERTKO_BsagRNA5tertko_2A-GFP

   Plasmid

   #198863

   Purpose

   Plasmid encoding for 2 gRNAs targeting the human TERT gene

   Depositor

   Agnel Sfeir

   Article

   Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

   Insert

   TERT sgRNA (TERT Human)
   

   Expression

   Mammalian

   Promoter

   u6

   Available Since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PE-pegRNA(BG)-nsgRNA(BG)

   Plasmid

   #232727

   Purpose

   A dual-targeting vector that contains PE3 system guide RNAs against BFP (pegRNA(BG) and nsgRNA(BG)) with restriction enzyme sites for insertion of target site guide RNAS (pegRNAs(TS) and nsgRNA(TS)).

   Depositor

   David Brafman

   Article

   Frisch et al Mol Ther Nucleic Acids. 2023 Jul 17;33:4

   Insert

   pegRNA(BG), nsgRNA(BG), pegRNAs(TS), nsgRNA(TS)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2

   Plasmid

   #74009

   Purpose

   Tissue-specific knock-out in zebrafish, Cas9 and GFP driven by a UAS promoter

   Depositor

   Filippo Del Bene

   Article

   Di Donato et al Genome Res. 2016 Mar 8.

   Inserts

   5x UAS
   Cas9
   T2A
   GFP
   U6 promoter
   

   Use

   CRISPR

   Available Since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUAS:Cas9T2ACre;U6:sgRNA1;U6:sgRNA2

   Plasmid

   #74010

   Purpose

   Tissue-specific knock-out in zebrafish, Cas9 and Cre driven by a UAS promoter

   Depositor

   Filippo Del Bene

   Article

   Di Donato et al Genome Res. 2016 Mar 8.

   Inserts

   5xUAS
   Cas9
   Cre
   U6 promoter
   

   Use

   CRISPR

   Available Since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Construct 3 - U6p_20nt1_mod2_sgRNA (stgRNA)

   Plasmid

   #81246

   Purpose

   For assaying self-targeting activity via Cas9

   Depositor

   Timothy Lu

   Article

   Perli et al Science. 2016 Aug 18. pii: aag0511.

   Insert

   sgRNA
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. U6-pegRNA-H1-nick sgRNA-mCherry

   Plasmid

   #176901

   Purpose

   Expresses prime editing guide RNA and guide RNA in mammalian cells

   Depositor

   Hyongbum Kim

   Article

   Jang et al Nat Biomed Eng. 2021 Aug 26. pii: 10.103

   Inserts

   mCherry
   gRNA scaffold
   gRNA scaffold
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG, H1, and U6

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJJF439_PU6_empty_sgRNAscaffold - sgRNA cloning backbone

    Plasmid

    #164266

    Purpose

    sgRNA cloning backbone for expression of sgRNA in C. elegans. Contains BbsI sites for insertion of spacer sequences. U6 promoter from W05B2.8 gene.

    Depositor

    Nikolaus Rajewsky

    Article

    Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Worm

    Promoter

    U6 promoter from W05B2.8

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LsgRNA-FXR1-KH1-gRNA

    Plasmid

    #225483

    Purpose

    Express gRNA for FXR1

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    gRNA for editing FXR1-KH1 (FXR1 Human)
    

    Use

    CRISPR

    Promoter

    hU6

    Available Since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TKIT NFL gRNA1 gRNA2

    Plasmid

    #182681

    Purpose

    Expression of spCas9, gRNA targeting intron 3 and gRNA targeting 3'UTR of the mouse Nefl gene. Can be used for the tagging of endogenous NFL via Targeted Knock-In with Two guides (TKIT) approach.

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Insert

    two Nefl-targeting gRNAs, one targets intron 3 and the other 3'UTR of the Nefl gene (Nefl Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 and chicken beta-actin promoter

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AAV-U6gRNA1-U6gRNA2-TnT-Cre

    Plasmid

    #87682

    Purpose

    AAV vector for U6 driven expression of two gRNAs, and cardiomyocyte specific expression of Cre recombinase.

    Depositor

    William Pu

    Article

    Guo et al Circ Res. 2017 Mar 29. pii: CIRCRESAHA.1

    Inserts

    gRNA1
    gRNA2
    Cre
    

    Use

    AAV

    Promoter

    U6 and cTnT

    Available Since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. px552-U6-gRNA1-U6-gRNA2-CMV-eGFP

    Plasmid

    #211760

    Purpose

    Paired gRNAs (sgRNA1 and 2) targeting Exon 1 of VEGFA gene conserved across mouse, rhesus macaque, and human.

    Depositor

    Glenn Yiu

    Article

    Chung et al Mol Ther Nucleic Acids. 2022 Apr 27;28:6

    Insert

    gRNA1 and gRNA2 targeting VEGF-A (VEGFA Mouse, Human, M. mulatta (rhesus macaque))
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSpCas9nucl-T2A-mCherry - BAKgRNA1- BAKgRNA2

    Plasmid

    #167295

    Purpose

    Plasmid encoding for 2 gRNAs targeting the human BAK gene and a CMV driven nuclease Cas9 followed by self-cleaving mCherry

    Depositor

    Agnel Sfeir

    Article

    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

    Insert

    BAK (BAK1 Human)
    

    Expression

    Mammalian

    Available Since

    April 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSpCas9nucl-T2A-EGFP - BAXgRNA1- BAXgRNA2

    Plasmid

    #167296

    Purpose

    Plasmid encoding for 2 gRNAs targeting the human BAX gene and a CMV driven nuclease Cas9 followed by self-cleaving EGFP

    Depositor

    Agnel Sfeir

    Article

    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

    Insert

    BAX (BAX Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PGL3-U6-SDHB_pegRNA-Csy4RS-nick_sgRNA-EGFP

    Plasmid

    #172670

    Purpose

    For expression of transcript containing pegRNA and nick-sgRNA targeting human SDHB gene from the U6 promoter with pegRNA flanked by Csy4 recognition site

    Depositor

    Xiaolong Wang

    Article

    Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142

    Insert

    SDHB pegRNA and SDHB_nick-sgRNA
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PGL3-U6-ALDOB_pegRNA-Csy4RS-nick_sgRNA-EGFP

    Plasmid

    #172668

    Purpose

    For expression of transcript containing pegRNA and nick-sgRNA targeting human ALDOB gene from the U6 promoter with pegRNA flanked by Csy4 recognition site

    Depositor

    Xiaolong Wang

    Article

    Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142

    Insert

    ALDOB pegRNA and ALDOB_nick-sgRNA
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PGL3-U6-RNF2_pegRNA-Csy4RS-nick_sgRNA-EGFP

    Plasmid

    #172669

    Purpose

    For expression of transcript containing pegRNA and nick-sgRNA targeting human RNF2 gene from the U6 promoter with pegRNA flanked by Csy4 recognition site

    Depositor

    Xiaolong Wang

    Article

    Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142

    Insert

    RNF2 pegRNA and RNF2_nick-sgRNA
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSG297-(Sp-gRNA)-(Sa-gRNA)-(PuroR_T2A_BFP(C>T screening))

    Plasmid

    #239448

    Purpose

    Orthogonal dual Cas9 gRNA and BFP reporter lentivirus cassette plasmid. For use with S. pyogenes and S. aureus gRNAs and includes a BFP reporter which reports on C-to-T editing.

    Depositor

    Alexis Komor

    Inserts

    S.p. gRNA backbone
    S.a. gRNA backbone
    PuroR-T2A-BFP(C>T_screening)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    BFP: T65S, S72S, V145F, K206K, H231L

    Promoter

    EF1alpha, U6, and mU6

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge