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pT7-EGFP-C1-CrPV-Rluc-6xbsMS2-PolyA-Malat_AC

Plasmid

#148446

Purpose

Mammalian Expression of 6xbsMS2-PolyA-Malat

Depositor

Elisa Izaurralde

Publication

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

6xbsMS2-PolyA-Malat

Expression

Mammalian

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HCV-RLuc-6xbsMS2-polyA-Malat_AC

Plasmid

#148448

Purpose

Mammalian Expression of 6xbsMS2-polyA-Malat

Depositor

Elisa Izaurralde

Publication

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

6xbsMS2-polyA-Malat

Expression

Mammalian

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HseIF1_AC

Plasmid

#148449

Purpose

Mammalian Expression of HseIF1

Depositor

Elisa Izaurralde

Publication

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HseIF1 (ELF1 Human)

Expression

Mammalian

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsRCK-E236Q-shRNAres_AC

Plasmid

#148450

Purpose

Mammalian Expression of HSRck-E236Q-shRNAres

Depositor

Elisa Izaurralde

Publication

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HSRck-E236Q-shRNAres (DDX6 Human)

Expression

Mammalian

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsRCK-K135A-shRNAres_AC

Plasmid

#148451

Purpose

Mammalian Expression of HsRck-K135A-shRNAres

Depositor

Elisa Izaurralde

Publication

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck-K135A-shRNAres (DDX6 Human)

Expression

Mammalian

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsRCK-QHTRRKKtoAAAAAAA-shRNAres_AC

Plasmid

#148452

Purpose

Mammalian Expression of HsRck-QHTRRKKtoAAAAAAA-shRNAres

Depositor

Elisa Izaurralde

Publication

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck-QHTRRKKtoAAAAAAA-shRNAres (DDX6 Human)

Expression

Mammalian

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsRCK-QHTRtoAAAA-shRNAres_AC

Plasmid

#148453

Purpose

Mammalian Expression of HsRck-QHTRtoAAAA-shRNAres

Depositor

Elisa Izaurralde

Publication

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck-QHTRtoAAAA-shRNAres (DDX6 Human)

Expression

Mammalian

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsRCK-RFQNRtoEDAAA-shRNAres_AC

Plasmid

#148454

Purpose

Mammalian Expression of HsRck-RFQNRtoEDAAA-shRNAres

Depositor

Elisa Izaurralde

Publication

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck-RFQNRtoEDAAA-shRNAres (DDX6 Human)

Expression

Mammalian

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-mCCK2R-stop

Plasmid

#179321

Purpose

Express mouse cholecystokinin 2 receptor (untagged) in mammalian cells

Depositor

Christian Gruber

Publication

Taghizadeh et al Sci Rep. 2022 Jun 2;12(1):9215. doi: 10.

Insert

mouse cholecystokinin2 receptor

Expression

Mammalian

Mutation

stop codon after ORF

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAB174

Plasmid

#101673

Purpose

Opto-T7RNAP*(179)

Depositor

Mustafa Khammash

Publication

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(179)

Use

Synthetic Biology

Expression

Bacterial

Available since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAB144

Plasmid

#101665

Purpose

Opto-T7RNAP*(69)

Depositor

Mustafa Khammash

Publication

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(69)

Use

Synthetic Biology

Expression

Bacterial

Available since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAB153

Plasmid

#101664

Purpose

Opto-T7RNAP*(600)

Depositor

Mustafa Khammash

Publication

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(600)

Use

Synthetic Biology

Expression

Bacterial

Available since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAB171

Plasmid

#101670

Purpose

Opto-T7RNAP*(69), equal expr.

Depositor

Mustafa Khammash

Publication

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(69), equal expr.

Use

Synthetic Biology

Expression

Bacterial

Available since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pSKA322

Plasmid

#101676

Purpose

empty backbone with chloramphenicol resistance

Depositor

Mustafa Khammash

Publication

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pPBO.ACT002

Plasmid

#182712

Purpose

aTc inducible dCasRx-IF1

Depositor

Joseph Schoeniger

Publication

Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

Insert

dCasRx

Use

CRISPR

Tags

3x(GGGS)-Escherichia Coli Initiation Factor 1

Expression

Bacterial

Mutation

Catalytically deactivated R295A, H300A, R849A, H8…

Promoter

pTet

Available since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pPBO.ACT004

Plasmid

#182714

Purpose

Constituitvely expressed CasRx crRNA cloning vector, extended 3' terminator region

Depositor

Joseph Schoeniger

Publication

Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

Insert

CasRx crRNA cloning backbone

Use

CRISPR

Expression

Bacterial

Mutation

Catalytically deactivated R295A, H300A, R849A, H8…

Promoter

pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)

Available since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-DmDCP1-GSSGmut-dsRNAres_Q

Plasmid

#147338

Purpose

Insect Expression of DmDCP1-GSSGmut-dsRNAres

Depositor

Elisa Izaurralde

Publication

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

DmDCP1-GSSGmut-dsRNAres (Dcp1 Fly)

Expression

Insect

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pCJH026_proD_Cas12c-multiplexing_CAM

Plasmid

#183073

Purpose

Plasmid expressing a Cas12c sgRNA targeting GFP and another sgRNA targeting RFP in one transcript for testing multiplexed repression

Depositor

Jennifer Doudna

Publication

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Expressing two Cas12c sgRNAs (targeting GFP or RFP) in one transcript

Use

CRISPR

Expression

Bacterial

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HCV-Fluc_AC

Plasmid

#148447

Purpose

Mammalian Expression of Fluc

Depositor

Elisa Izaurralde

Publication

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

Fluc

Expression

Mammalian

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCIneo-Rluc-6xbsMS2-random_AC

Plasmid

#148431

Purpose

Mammalian Expression of 6xbsMS2-random

Depositor

Elisa Izaurralde

Publication

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

6xbsMS2-random

Expression

Mammalian

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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