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RNAPII_CTD27-52_N2A_S1966C

Plasmid

#98682

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with N2A and S1966C

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Tags

6xHis

Expression

Bacterial

Mutation

N1775A, N1782A, S1966C

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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RNAPII_CTD27-52_K5S_S1966C

Plasmid

#98683

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with K5S and S1966C

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Tags

6xHis

Expression

Bacterial

Mutation

K1838S, K1859S, K1866S, K1873S, K1887S, S1966C

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE/SP M1 S245X

Plasmid

#92372

Purpose

Inducible expression of M1S245X spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-194 bp of 5'UTR deleted, S245X

Promoter

Tet-responsive P tight

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/M87 N184X

Plasmid

#92367

Purpose

expression of M87 N184X spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-460 bp deleted, N184X

Promoter

CMV

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/M1 N184X

Plasmid

#92365

Purpose

expression of M1 N184X spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-194 bp of 5'UTR deleted, N184X

Promoter

CMV

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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isoform PKC Epsilon (JCE592)

Plasmid

#89776

Purpose

yeast expression of mouse PKC Epsilon with yeast PKC1 promoter and ADH1 terminator

Depositor

Juan Carlos Igual

Article

Soriano-Carot et al Nucleic Acids Res. 2014 Jun;42(11):7084-

Insert

PKC Epsilon (Prkce Mouse)

Expression

Mammalian

Promoter

yeast PKC1 promoter (-600 to -1)

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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isoform PKC Eta (JCE615)

Plasmid

#89774

Purpose

yeast expression of mouse PKC Eta with yeast PKC1 promoter and ADH1 terminator

Depositor

Juan Carlos Igual

Article

Soriano-Carot et al Nucleic Acids Res. 2014 Jun;42(11):7084-

Insert

PKC Eta (Prkch Mouse)

Expression

Mammalian

Promoter

yeast PKC1 promoter (-600 to -1)

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pHU6_Ch5_155183064-gRNA-for

Plasmid

#81214

Purpose

pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 3' region of pCALNL_Ch5 reporter

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA

Expression

Mammalian

Promoter

U6

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pHU6_Ch5_155183064-gRNA-rev

Plasmid

#81215

Purpose

pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 5' region of pCALNL_Ch5 reporter

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA

Expression

Mammalian

Promoter

U6

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP C1 SADBs shRNA Resistant

Plasmid

#67155

Purpose

pEGFP containing N-terminally EGFP Tagged mouse SADBs resistant to an shRNA

Depositor

Maria Alvarado-Kristensson, Ana Carrera

Article

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

Brsk1 (Brsk1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

nucleotide 294 C-T, nucleotide 297 C-G

Promoter

CMV

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only
pSG5 N-HA SADBs shRNA resistant

Plasmid

#67157

Purpose

N-terminally HA tagged mouse SADBs containing substitutions providing resistance to an shRNA

Depositor

Maria Alvarado-Kristensson, Ana Carrera

Article

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

Brsk1 (Brsk1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Nucleotide 294 C-T Nucleotide 297 C-G (shRNA resi…

Promoter

SV40 promoter

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only
pTER SADB shRNA Human

Plasmid

#67151

Purpose

Doxycycline-regulated mammalian expression vector for expressing shRNA against human SADB

Depositor

Maria Alvarado-Kristensson, Ana Carrera

Article

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

BRSK1 (BRSK1 Human)

Use

RNAi

Expression

Mammalian

Promoter

H1 Tet

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only
pFRT-TODestFLAGHA_RBPMS_KDR

Plasmid

#65091

Purpose

Destination vector with RBPMS resistant to siRNA treatment by s21729 (Applied Biosystems) for stable cell line generation

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS (RBPMS Human)

Tags

FLAG HA

Expression

Mammalian

Mutation

changed sequence to render plasmid resistant to s…

Promoter

CMV, 2x Tet

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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AI-CN491

Plasmid

#62594

Purpose

TALEN array receiving vector with half-site NN RVD

Depositor

Joshua Grimley

Article

Martinez et al Nucleic Acids Res. 2015 Mar 11. pii: gkv

Type

Empty backbone

Use

TALEN; T7

Tags

3× FLAG and FOKI WT

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2015

Availability

Academic Institutions and Nonprofits only

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AI-CN443

Plasmid

#62593

Purpose

TALEN array receiving vector with half-site NG RVD

Depositor

Joshua Grimley

Article

Martinez et al Nucleic Acids Res. 2015 Mar 11. pii: gkv

Type

Empty backbone

Use

TALEN; T7

Tags

3× FLAG and FOKI WT

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2015

Availability

Academic Institutions and Nonprofits only

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AI-CN442

Plasmid

#62591

Purpose

TALEN array receiving vector with half-site HD RVD

Depositor

Joshua Grimley

Article

Martinez et al Nucleic Acids Res. 2015 Mar 11. pii: gkv

Type

Empty backbone

Use

TALEN; T7

Tags

3× FLAG and FOKI WT

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2015

Availability

Academic Institutions and Nonprofits only

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AI-CN441

Plasmid

#62590

Purpose

TALEN array receiving vector with half-site NI RVD

Depositor

Joshua Grimley

Article

Martinez et al Nucleic Acids Res. 2015 Mar 11. pii: gkv

Type

Empty backbone

Use

TALEN; T7

Tags

3× FLAG and FOKI WT

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2015

Availability

Academic Institutions and Nonprofits only

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p416GAL1-Skn7t-L-IFP-F[1] (Yeast)

Plasmid

#52896

Purpose

Skn7t replaces the zipper and is fused to the IFP-F[1] through the linker (GGGGS) X 2. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

Skn7t-L-IFP-F[1]

Tags

L-IFP-F[1]

Expression

Yeast

Promoter

GAL1

Available Since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-MAVS-M1A500(KaganF60)

Plasmid

#52003

Purpose

Expresses the human MAVS CDS containing a point mutation M1A and a C-terminal deletion removing the transmembrane domain

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS-M1A-500

Expression

Mammalian

Mutation

changed Met 1 to Ala and deleted the C-terminal t…

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
1132 pME-Lin28

Plasmid

#49009

Purpose

full length coding sequence for zebrafish lin28 gene in gateway middle entry vector

Depositor

Nathan Lawson

Article

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

lin-28 homolog A (lin28ab Zebrafish)

Use

Gateway middle entry

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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