Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 4,862 results for: Mela

Showing: 4801 - 4820 of 4862 results

  3. pRRL-SFFV-rtTA3-IRES-Luc-P2A-mtagBFP

    Plasmid
    #176027
    Purpose
    construct expresses the reverse tet-responsive transactivator 3 (rtTA3) together with Luciferase (Luc) for bioluminescence in vivo imaging, and mTaqBFP for enriching transgenic cells by flow cytometry
    Depositor
    Irmela Jeremias
    Article
    Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1
    Inserts
    rtTA3
    Luc2
    mtagBFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Jan. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    176027-plasmid-map-sequence-id-351264

    Enlarge

  5. MCHR1-DuET

    Plasmid
    #213337
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    MCHR1 (MCHR1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213337-plasmid-map-sequence-id-430912

    Enlarge

  6. LLPS1-iLID::EGFP::FTH1 (pBS1042)

    Plasmid
    #185289
    Purpose
    For the mammalian expression of the synthetic protein iLID::eGFP::FTH1 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation
    Depositor
    Pamela Silver
    Article
    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1
    Insert
    iLID::eGFP::FTH1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185289-plasmid-map-sequence-id-363896

    Enlarge

  12. MC2R-DuET

    Plasmid
    #213336
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    MC2R (MC2R Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213336-plasmid-map-sequence-id-430911

    Enlarge

  13. MCHR2-DuET

    Plasmid
    #213338
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    MCHR2 (MCHR2 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213338-plasmid-map-sequence-id-430867

    Enlarge

  14. PvLEA4_repeats_k3_26_mCh-SspB (pBS1075)

    Plasmid
    #185299
    Purpose
    Mammalian expression of sleeping chironomid protein PvLEA4_repeats_k3_26 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation.
    Depositor
    Pamela Silver
    Article
    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1
    Insert
    pvLEA22mer_shuffle_3
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    July 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    185299-plasmid-map-sequence-id-363926

    Enlarge

  15. DHN_VrDHN1a_mCh-SspB (pBS1074)

    Plasmid
    #185298
    Purpose
    Mammalian expression of riverbank grape plant protein DHN_VrDHN1a attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation.
    Depositor
    Pamela Silver
    Article
    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1
    Insert
    PvLEA4_repeats_k3_26
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    July 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    185298-plasmid-map-sequence-id-363925

    Enlarge

  16. ApoE3_D125I_47-300_mCh-SspB (pBS1071)

    Plasmid
    #185294
    Purpose
    For the mammalian expression of the human protein ApoE3_D125I_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation
    Depositor
    Pamela Silver
    Article
    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1
    Insert
    ApoE3_D125I_47-300
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D125I
    Promoter
    Available Since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    185294-plasmid-map-sequence-id-363903

    Enlarge

  20. pvLEA22mer_mutant_3_mCh-SspB (pBS1080)

    Plasmid
    #185303
    Purpose
    For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_3 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation
    Depositor
    Pamela Silver
    Article
    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1
    Insert
    pvLEA22mer_mutant_3
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185303-plasmid-map-sequence-id-363934

    Enlarge

Showing: 4801 - 4820 of 4862 results