We narrowed to 10,183 results for: yeast

1. pCfB8622

   Plasmid

   #126912

   Purpose

   Plasmid containing gRNA expression cassette targeting ADE2 in Saccharomyces cerevisiae

   Depositor

   Irina Borodina

   Article

   Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

   Insert

   ADE2 gRNA
   

   Use

   CRISPR

   Available Since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYZ675

   Plasmid

   #132954

   Purpose

   crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with dFnCas12a Schizosaccharomyces pombe

   Depositor

   Jef Boeke

   Article

   Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578

   Insert

   dFnCas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   nucleoplasmin NLS

   Promoter

   adh1

   Available Since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYZ694

   Plasmid

   #132956

   Purpose

   crRNA entry vector of Prrk1-Not1-HHR with dFnCas12a-Clr4 in Schizosaccharomyces pombe

   Depositor

   Jef Boeke

   Article

   Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578

   Insert

   dFnCas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40 NLS and nucleoplasmin NLS

   Promoter

   adh1

   Available Since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDH6

   Plasmid

   #83777

   Purpose

   Useful for tagging yeast proteins with C-terminal YFP tag; G418 selection

   Depositor

   Eric Muller

   Article

   Hailey et al Methods Enzymol. 2002;351:34-49.

   Insert

   YFP
   

   Tags

   YFP

   Expression

   Yeast

   Mutation

   S65G, V68L, Q69K, S72A, T203Y mutations added.

   Available Since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. jk089_h12

   Plasmid

   #112151

   Purpose

   C. thermophilum DASH/Dam1 complex (construct used for cryo-EM structure determination)

   Depositor

   Stephen Harrison

   Article

   Jenni et al Science. 2018 May 4;360(6388):552-558. d

   Insert

   C. thermophilum DASH/Dam1 complex
   

   Tags

   his tag on Spc34 and strep tag on Dad1

   Available Since

   Sept. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. p184.3

   Plasmid

   #44521

   Depositor

   Michael Grunstein

   Article

   Hecht et al Cell. 1995 Feb 24;80(4):583-92.

   Insert

   Histone 3 N-terminus
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa1-46

   Available Since

   Oct. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pRS313-Sad1-Wt

   Plasmid

   #90308

   Purpose

   yeast expression

   Depositor

   Christine Guthrie

   Article

   Hadjivassiliou et al RNA. 2014 May;20(5):656-69. doi: 10.1261

   Insert

   SAD1
   

   Tags

   no tag

   Expression

   Yeast

   Available Since

   June 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDZ264 (pKAN tdtomato cyc loxP KAN loxP)

   Plasmid

   #35193

   Depositor

   Robert Singer

   Article

   Larson et al Science. 2011 Apr 22;332(6028):475-8.

   Insert

   tdtomato cyc loxP KAN loxP
   

   Available Since

   March 5, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAc-Cdc3-Cdc10

   Plasmid

   #131160

   Purpose

   Expression of Septin rods

   Depositor

   Nils Johnsson

   Article

   Renz et al BMC Biotechnol. 2013 Jul 26;13:60. doi:

   Insert

   Cdc3 + Cdc10
   

   Use

   Bicistronic

   Tags

   Cdc10: S Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYCTK009

    Plasmid

    #176713

    Purpose

    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor gene of Eremothecium cymbalariae (sender part).

    Depositor

    Victor Sourjik

    Article

    Krink et al bioRxiv 2021

    Insert

    mfalpha1Ec
    

    Use

    Synthetic Biology

    Mutation

    Codon-optimized for expression in S. cerevisiae

    Available Since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYCTK020

    Plasmid

    #176724

    Purpose

    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Ste2 gene of Eremothecium cymbalariae (receiver part).

    Depositor

    Victor Sourjik

    Article

    Krink et al bioRxiv 2021

    Insert

    ste2Ec
    

    Use

    Synthetic Biology

    Mutation

    Codon-optimized for expression in S. cerevisiae

    Available Since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYCTK030

    Plasmid

    #176734

    Purpose

    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Bar1 gene of Candida albicans (barrier part).

    Depositor

    Victor Sourjik

    Article

    Krink et al bioRxiv 2021

    Insert

    bar1Ca
    

    Use

    Synthetic Biology

    Mutation

    Codon-optimized for expression in S. cerevisiae

    Available Since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYCTK031

    Plasmid

    #176735

    Purpose

    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Bar1 gene of Eremothecium cymbalariae (barrier part).

    Depositor

    Victor Sourjik

    Article

    Krink et al bioRxiv 2021

    Insert

    bar1Ec
    

    Use

    Synthetic Biology

    Mutation

    Codon-optimized for expression in S. cerevisiae

    Available Since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYCTK008

    Plasmid

    #176712

    Purpose

    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor gene of Candida albicans (sender part).

    Depositor

    Victor Sourjik

    Article

    Krink et al bioRxiv 2021

    Insert

    mfalpha1Ca
    

    Use

    Synthetic Biology

    Mutation

    Codon-optimized for expression in S. cerevisiae

    Available Since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRS406-PHO5-GFP-Scs2

    Plasmid

    #58834

    Purpose

    Expresses GFP-Tagged Scs2 in yeast

    Depositor

    Tim Levine

    Article

    Loewen et al EMBO J. 2003 May 1;22(9):2025-35.

    Insert

    Scs2 (SCS2 Budding Yeast)
    

    Tags

    GFP and Myc

    Expression

    Yeast

    Promoter

    PHO5

    Available Since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

16. LHP308 - Ub - wt

    Plasmid

    #32151

    Depositor

    Linda Hicke

    Article

    Terrell et al Mol Cell. 1998 Jan;1(2):193-202.

    Insert

    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    

    Expression

    Yeast

    Promoter

    CUP1

    Available Since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

17. YIp204-P30-His-POL30

    Plasmid

    #99550

    Purpose

    Yeast integrative vector for expression of His-tagged POL30 (PCNA); TRP1 marker

    Depositor

    Helle Ulrich

    Article

    Davies et al Methods Mol Biol. 2012;920:543-67. doi:

    Insert

    POL30 (POL30 Budding Yeast)
    

    Tags

    6xHis

    Expression

    Yeast

    Promoter

    POL30

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAS2-1-Esf1

    Plasmid

    #99987

    Purpose

    Express yeast Esf1 with Y2H binding domain fusion

    Depositor

    Susan Baserga

    Article

    Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

    Insert

    Esf1 (ESF1 Budding Yeast)
    

    Tags

    Y2H binding domain

    Expression

    Yeast

    Available Since

    Nov. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. SL-25-0

    Plasmid

    #108673

    Purpose

    in vitro mRNA transcription

    Depositor

    Jon Lorsch

    Article

    Yourik et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

    Insert

    CAA repeat mRNA
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAEF6

    Plasmid

    #136303

    Purpose

    pAEF5 plasmid with two gRNA inserted

    Depositor

    Gilles Fischer

    Article

    Agier et al Methods Mol Biol. 2021;2196:181-198. doi

    Insert

    double cassette coding for gRNA
    

    Use

    CRISPR; Template for pcr to insert double grna co…

    Available Since

    Feb. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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