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pDH006

Plasmid

#157641

Purpose

sMHT-113 fused SYNZIP18 only

Depositor

Jonathan Silberg

Publication

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Inserts

sMHT(1-113)
SYNZIP18-sMHT(114-23)

Expression

Bacterial

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDH007

Plasmid

#157642

Purpose

sMHT-113 fused to CheA and CheY

Depositor

Jonathan Silberg

Publication

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Inserts

sMHT(1-113) -CheA
CheY-sMHT(114-23)

Expression

Bacterial

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDH008

Plasmid

#157643

Purpose

sMHT-113 fused to CheY only

Depositor

Jonathan Silberg

Publication

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Inserts

sMHT(1-113)
CheY-sMHT(1-114)

Expression

Bacterial

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDH011

Plasmid

#157646

Purpose

sMHT-113 fused to FKBP/FRB

Depositor

Jonathan Silberg

Publication

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Inserts

sMHT(1-113)-FKBP
FRB-sMHT(114-230)

Expression

Bacterial

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDH012

Plasmid

#157647

Purpose

Constitutive sMHT-113-SYNZIP expression

Depositor

Jonathan Silberg

Publication

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Inserts

sMHT(1-113)-SYNZIP17
SYNZIP18-sMHT(114-230)

Expression

Bacterial

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pGMR-WhiteA2

Plasmid

#165874

Purpose

Basic transgenesis 5xGMR white CDS physical eye marker cassette in Alpha2, lacks attB site

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Eye Marker Cassette

Use

Synthetic Biology

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pT2U2-YFP

Plasmid

#84544

Purpose

Hybrid reporter construct with 4 transcription factor binding sites (2 TetO, 2 UAS) driving EYFP

Depositor

Joshua Leonard

Publication

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

T2U2-EYFP

Expression

Mammalian

Promoter

CMV-min

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pT2U3-YFP

Plasmid

#84547

Purpose

Hybrid reporter construct with 5 transcription factor binding sites (2 TetO, 3 UAS) driving EYFP

Depositor

Joshua Leonard

Publication

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

T2U3-EYFP

Expression

Mammalian

Promoter

CMV-min

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pU2T4-YFP

Plasmid

#84548

Purpose

Hybrid reporter construct with 6 transcription factor binding sites (2 UAS, 4 TetO) driving EYFP

Depositor

Joshua Leonard

Publication

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

U2T4-EYFP

Expression

Mammalian

Promoter

CMV-min

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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Rap-MESA-20-Tev

Plasmid

#84555

Purpose

MESA protease chain with rapamycin FKBP ectodomain, 20 extracellular linkers, and Tev protease

Depositor

Joshua Leonard

Publication

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

Rap-MESA-FKBP-20-Tev

Use

AAV

Expression

Mammalian

Promoter

CMV

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pU2T2-YFP

Plasmid

#84543

Purpose

Hybrid reporter construct with 4 transcription factor binding sites (2 UAS, 2 TetO) driving EYFP

Depositor

Joshua Leonard

Publication

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

U2T2-EYFP

Expression

Mammalian

Promoter

CMV-min

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCSC-CMV-HA-Ast-3-NP-1-IRES-mCherry

Plasmid

#159631

Purpose

expresses HA Allatostatin-3, Neurophysin-1, IRES mCherry under the CSC promoter

Depositor

Andrew Allen, Ross Bathgate

Publication

Ngo et al Cell Rep. 2020 Sep 15;32(11):108139. doi

Insert

Ast (AstA Fly)

Use

Lentiviral

Tags

HA, IRES, mCherry, and neurophysin-1

Expression

Mammalian

Promoter

CMV promoter

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pMJA008

Plasmid

#128512

Purpose

Expresses Xylanase C

Depositor

Marcos Alcocer

Publication

Furniss et al Biochim Biophys Acta. 2002 Jul 29;1598(1

Insert

Xylanase C

Expression

Bacterial

Promoter

Histone 4B

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI119

Plasmid

#126059

Purpose

Module 0 vector of PlaMiNGo toolkit

Depositor

Ralf-Bernd Klösgen

Publication

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

chloroplastic FNR transit peptide

Use

Synthetic Biology; Module 0

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI120

Plasmid

#126060

Purpose

Module 0 vector of PlaMiNGo toolkit

Depositor

Ralf-Bernd Klösgen

Publication

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

mitochondria Rieske iron sulphur protein (1-100 aa)

Use

Synthetic Biology; Module 0

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI123

Plasmid

#126063

Purpose

Module 0 vector of PlaMiNGo toolkit

Depositor

Ralf-Bernd Klösgen

Publication

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

GFP 11x3 (5 aa linker)

Use

Synthetic Biology; Module 0

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI124

Plasmid

#126064

Purpose

Module 0 vector of PlaMiNGo toolkit

Depositor

Ralf-Bernd Klösgen

Publication

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

GFP 11x7 (5 aa linker)

Use

Synthetic Biology; Module 0

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI125

Plasmid

#126065

Purpose

Module 0 vector of PlaMiNGo toolkit

Depositor

Ralf-Bernd Klösgen

Publication

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

YFP1-10

Use

Synthetic Biology

Tags

linker (GTGGGGS)

Mutation

T205Y

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR sgAMDHD1

Plasmid

#102316

Purpose

genetic depletion of AMDHD1

Depositor

David Sabatini

Publication

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

sgAMDHD1 (AMDHD1 Human)

Use

Lentiviral

Promoter

U6

Available since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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Hsh155 N4A 3xFLAG

Plasmid

#111975

Purpose

Shuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker. It has 3xFLAG at the C-terminus. Contains the N4A mutation.

Depositor

Aaron Hoskins

Publication

Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d

Insert

Hsh155 N4A 3x FLAG

Tags

3xFLAG

Expression

Bacterial and Yeast

Mutation

T178A R181A R182A R186A

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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