We narrowed to 6,411 results for: kit

1. pET28a(+)::MBP-NL3F10H

   Plasmid

   #141291

   Purpose

   MBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

   Depositor

   Andrew Millar

   Article

   Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

   Insert

   MBP-NLUC3F10H
   

   Use

   Luciferase

   Tags

   Flag (x3), His (x10), and MBP

   Expression

   Bacterial

   Mutation

   Engineered for high stability (t1/2 = 11.5 days a…

   Promoter

   promoter for bacteriophage T7 RNA polymerase

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_EYFP

   Plasmid

   #167898

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMXs-p53P275S

   Plasmid

   #22726

   Depositor

   Shinya Yamanaka

   Article

   Hong et al Nature. 2009 Aug 27. 460(7259):1132-5.

   Insert

   Trp53 (Trp53 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   P275S

   Promoter

   Available since

   Jan. 4, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pICH47802-p35S::tdTomato-ER::tNOS

   Plasmid

   #160220

   Purpose

   tdTomato fluorescent protein-ER localization, 35 promoter, tNOS terminator, transcriptional unit, inserted at the MoClo toolkit level 1 acceptor position 1 reverse

   Depositor

   Matias Kirst

   Article

   Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

   Insert

   p2x35S::TdTomato-ER::tNOS
   

   Use

   Synthetic Biology; Moclo toolkit level 1 acceptor…

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLYS1-EMRE/RNAi resistant

   Plasmid

   #50056

   Purpose

   Expression of human EMRE in mammalian cells with silent mutation making it resistant to RNAi

   Depositor

   Vamsi Mootha

   Article

   Sancak et al Science. 2013 Nov 14.

   Insert

   EMRE-RNAi resistant (SMDT1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   RNAi resistant, silent mutant

   Promoter

   Available since

   Feb. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMXs-Scl

   Plasmid

   #131601

   Purpose

   expression of mouse ScI in mammalian cells

   Depositor

   Filipe Pereira, Kateri Moore

   Article

   Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

   Insert

   Scl (Tal1 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Perox-B

   Plasmid

   #209867

   Purpose

   Dimerization dependent fluorescent protein B anchored to cytosolic face of the peroxisomal membrane with N-terminal targeting sequence of human PEX3

   Depositor

   Sarah Cohen

   Article

   Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

   Insert

   ddGFP B
   

   Use

   Tags

   Targeting domain of PEX3 MLRSVWNFLKRHKKKCIFLGTVLG…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDB22

   Plasmid

   #61342

   Purpose

   Mammalian expression of mCherry fused to LINuS, a light-inducible nuclear localization signal (cMycP1A NLS/PKIt NES)

   Depositor

   Barbara Di Ventura, Roland Eils

   Article

   Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

   Insert

   mCherry
   

   Use

   Tags

   GS linker-AsLov2-cMyc1A NLS and PKIt NES

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMOD_B2518

   Plasmid

   #91075

   Purpose

   Module B, Promoter: TaU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Esp3I ccdb cassette for single gRNA spacer cloning
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   TaU6

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBTK402

    Plasmid

    #110598

    Purpose

    BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    mRFP dropout (RSF1010 origin)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMOD_C2906

    Plasmid

    #91093

    Purpose

    Module C, Promoter: FMV 34S, Gene: TREX2 (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TREX2 (+ BaeI site for GT donor cloning) (Trex2 Mouse)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    FMV 34S

    Available since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. NAX_CAGGS_CAS9_m4_VPR_mcherry

    Plasmid

    #167886

    Purpose

    PiggyBac compatible plasmid expressing dCas9-VPR

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

13. pTC364

    Plasmid

    #91213

    Purpose

    protoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, Csy4 processing)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    2 gRNAs targeting tomato ARF8A
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMXs-ms-Sox18

    Plasmid

    #50782

    Purpose

    retroviral expression of mouse Sox18

    Depositor

    Shinya Yamanaka

    Article

    Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

    Insert

    Sox18 (Sox18 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYSD300

    Plasmid

    #212930

    Purpose

    Encodes the S. cerevisiae Aga1 protein (Part3) with pGBK1 promoter, tENO2 terminator, HIS3 selectable marker and CEN/ARS yeast origin.

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    AGA1 (AGA1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBTK501

    Plasmid

    #110602

    Purpose

    BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (ampicillin resistance)

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTC394

    Plasmid

    #91224

    Purpose

    protoplast vector expressing gRNA24 targeting tomato ANT1 (control without TREX2 expression)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    gRNA targeting tomato ANT1
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTC392

    Plasmid

    #91223

    Purpose

    protoplast vector expressing gRNA23 targeting tomato ANT1 (control without TREX2 expression)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    gRNA targeting tomato ANT1
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBTK229

    Plasmid

    #110591

    Purpose

    BTK Type 3 coding sequence for golden gate assembly and homologous recombination

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    Kanamycin resistance
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pBTK570

    Plasmid

    #110615

    Purpose

    BTK assembled broad-host-range plasmid encoding E2-Crimson protein driven by PA3 promoter

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    E2-Crimson
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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