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We narrowed to 7,094 results for: pcdna3.1

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Showing: 4841 - 4860 of 7094 results

  1. mGFAP-Halo

    Plasmid
    #169474
    Purpose
    the host vector was obtained by substitution of the EGFP tag from a pEGFP-N3-mGFAP plasmid for a HaloTag using BamHI and NotI restriction sites
    Depositor
    Federico Herrera
    Publication
    Letra-Vilela et al Experimental Results (2020), Volume 1, e
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GFAP (Gfap Mouse)
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Changed R 236 to H
    Available since
    June 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    169474-plasmid-map-sequence-id-334721

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  2. pspCas9-HF-tetra-com-vector

    Plasmid
    #132555
    Purpose
    Vector plasmid expressing high fidelity spCas9 (R691A) and sgRNA scaffold with com replacing the Tetraloop.
    Depositor
    Baisong Lu
    Publication
    Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    High fidelity Cas9
    sgRNA scaffold with com replacing the Tetraloop
    Expression
    Mammalian
    Available since
    March 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    132555-plasmid-map-sequence-id-259534

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  4. pspCas9-ABE-3'UTR-sgRNA-Tetra-com-vector

    Plasmid
    #132560
    Purpose
    Vector plasmid expressing ABEmax and sgRNA scaffold with com replacing the Tetraloop.
    Depositor
    Baisong Lu
    Publication
    Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    ABEmax
    sgRNA, with Tetraloop replaced by com aptamer
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    132560-plasmid-map-sequence-id-259542

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  5. pcDNA3-PPO-Venus

    Plasmid
    #139502
    Purpose
    Expresses Venus-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.
    Depositor
    Michael Bruchas
    Publication
    Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    parapinopsin
    Tags
    Venus tag following 3x Ala linker
    Expression
    Mammalian
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    139502-plasmid-map-sequence-id-288143

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  7. FKBP-CD28TMD-NTEVp(H75S)

    Plasmid
    #137828
    Purpose
    MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75S mutant TEV protease
    Depositor
    Joshua Leonard
    Publication
    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FKBP-CD28TMD-NTEVp(H75S)
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    Mutated Histidine 75 to Serine in NTEVp fragment
    Promoter
    CMV
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    137828-plasmid-map-sequence-id-311924

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  10. pCMV-ABEMAX-NLS-P2A_BLAST

    Plasmid
    #135355
    Purpose
    Expression of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance
    Depositor
    Alex Hewitt
    Publication
    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ABEMax-2XNLS
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    WT
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    135355-plasmid-map-sequence-id-267704

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  11. pcDNA3-HCMVgO-sfGFP (TB40/E)

    Plasmid
    #136451
    Purpose
    Mammalian expression plasmid for HCMV glycoprotein O (TB40/E strain) fused to superfolder GFP
    Depositor
    Erik Procko
    Publication
    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    glycoprotein O (UL74 Human betaherpesvirus 5)
    Tags
    Gly/Ser-rich linker (GGSGGSGSGG) (includes BamHI …
    Expression
    Mammalian
    Mutation
    Codon-optimized for human cell expression.
    Promoter
    CMV
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    136451-plasmid-map-sequence-id-271690

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  12. pcDNA3-HCMVgH-8his (TB40/E)

    Plasmid
    #136445
    Purpose
    Mammalian expression plasmid for HCMV glycoprotein H (TB40/E strain) extracellular domain with 8his purification tag
    Depositor
    Erik Procko
    Publication
    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.
    Insert
    glycoprotein H (UL75 Human betaherpesvirus 5)
    Tags
    Short GGSG linker and 8his tag
    Expression
    Mammalian
    Mutation
    Extracellular domain (a.a. 1-717). Codon-optimize…
    Promoter
    CMV
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    136445-plasmid-map-sequence-id-269879

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  13. pcDNA3-HCMVgH (TB40/E)

    Plasmid
    #136446
    Purpose
    Mammalian expression plasmid for HCMV glycoprotein H (TB40/E strain), full-length
    Depositor
    Erik Procko
    Publication
    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    glycoprotein H (UL75 Human betaherpesvirus 5)
    Expression
    Mammalian
    Mutation
    Codon-optimized for human cell expression.
    Promoter
    CMV
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    136446-plasmid-map-sequence-id-271694

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  14. pcDNA3-sACE2-WT(732)

    Plasmid
    #154098
    Purpose
    Mammalian expression plasmid for dimeric soluble ACE2 (untagged)
    Depositor
    Erik Procko
    Publication
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease and neck domains …
    Promoter
    CMV
    Available since
    June 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    154098-plasmid-map-sequence-id-304580

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  16. mCerulean-hCOL1A1

    Plasmid
    #140112
    Purpose
    Expresses fluorescently-tagged human Type I procollagen α1 chain with mCerulean replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Sergey Leikin
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Type I procollagen α1 (COL1A1 Human)
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    COL1A1 exons 2-3 replaced by fluorescent tag
    Promoter
    CMV
    Available since
    May 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    140112-plasmid-map-sequence-id-296778

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  17. pcDNA3-sACE2v2-Fc(IgA1)

    Plasmid
    #145157
    Purpose
    Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) high affinity variant 2
    Depositor
    Erik Procko
    Publication
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Fc region of human IgA1
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    145157-plasmid-map-sequence-id-292593

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  18. pcDNA3-sACE2v3-sfGFP

    Plasmid
    #145174
    Purpose
    Mammalian expression plasmid for sACE2-sfGFP high affinity variant 3
    Depositor
    Erik Procko
    Publication
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Linker GSGGSGSGG and superfolder GFP
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    145174-plasmid-map-sequence-id-292600

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  19. HA-RNF126 C231A

    Plasmid
    #138648
    Purpose
    Mammalian expression of full-length2 HA-tagged RNF126 with an inactivating mutation in the RING domain (C231A)
    Depositor
    Jane McGlade
    Publication
    Smith et al Exp Cell Res. 2014 Jan 15;320(2):219-32.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RNF126 (RNF126 Mouse)
    Tags
    HA-HA
    Expression
    Mammalian
    Mutation
    C231A
    Available since
    Feb. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    138648-plasmid-map-sequence-id-273239

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Showing: 4841 - 4860 of 7094 results