We narrowed to 75,279 results for: plasmid

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pAAV-R-Creon shRNA[Control]

Plasmid

#180775

Purpose

This vector simultaneously expresses mCherry protein and shRNA in a Cre-dependent manner.

Depositor

Eun Mi Hwang

Article

Kim et al J Neurosci Methods. 2021 Dec 21:109452.

Type

Empty backbone

Use

AAV, Cre/Lox, and RNAi

Expression

Mammalian

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-Syn-PSD95.FingR-eGFP-CCR5TC

Plasmid

#125693

Purpose

Global labeling of excitatory synapses (green fluorescence)

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

PSD95.FingR-eGFP-CCR5TC

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV SCH9

Plasmid

#212708

Purpose

A SCHEMA evolved AAV variant effective at targeting subventricular NSCs

Depositor

David Schaffer

Article

Ojala et al Mol Ther. 2018 Jan 3;26(1):304-319. doi:

Insert

AAV2 Rep SCH9 Cap

Use

AAV

Available Since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pArk313

Plasmid

#200257

Purpose

Ark313 rep/cap

Depositor

Justin Eyquem

Article

Nyberg et al Cell. 2023 Jan 19;186(2):446-460.e19. do

Insert

Ark313 Rep/Cap

Use

AAV

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-PE6a

Plasmid

#207851

Purpose

Mammalian expression of PE6a prime editor

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PE6a

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

Ec48rt(E60K, K87E, E165D, D243N, R267I, E279K, K3…

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-CMVc-Cas9

Plasmid

#106431

Purpose

Expresses SpCas9 from the CMVc promoter in AAV backbone

Depositor

Juan Belmonte

Article

Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

Insert

Cas9

Use

AAV and CRISPR

Tags

HA-NLS and NLS

Expression

Mammalian

Promoter

CMVc

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-fDIO-JRGECO1a

Plasmid

#128317

Purpose

Expresses JRGECO1a under control of eukaryotic Ef1a promoter after Flp-dependent recombination.

Depositor

Patricia Jensen

Has Service

AAV1

Insert

NES-JRGECO1a

Use

AAV

Tags

His Tag

Expression

Mammalian

Promoter

Ef1a

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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MOS

Plasmid

#64120

Purpose

A modified episomal (EBNA1/oriP) vector expressing human OCT4 and SOX2 genes

Depositor

Linzhao Cheng

Article

Chou et al Stem Cells Transl Med. 2015 Mar 5. pii:

Insert

OCT4-2A-SOX2 (POU5F1, SOX2 Human)

Tags

HA and Myc

Expression

Mammalian

Promoter

SFFV

Available Since

Oct. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pCSC-NGN2-IRES-GFP-T2A-Sox11

Plasmid

#90214

Purpose

To convert human skin fibroblasts into induced motor neurons (hiMN) in combination with ISL1, LHX3, FGF2 and two small molecules, forskolin and dorsomorphin.

Depositor

Chun-Li Zhang

Article

Liu et al Cell Rep. 2016 Jan 5;14(1):115-28. doi:

Insert

NGN2-IRES-GFP-T2A-Sox11 (NEUROG2, Sox11 Mouse, Human)

Use

Lentiviral

Promoter

CMV

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-flex-dsRed-shvgat

Plasmid

#67845

Purpose

Expresses shRNA to the vesicular GABA transporter VGAT. Cre dependent expression by flex switch

Depositor

William Wisden

Article

Yu et al Neuron. 2015 Jun 17. pii: S0896-6273(15)

Insert

AAV-hsyn-flexed-dsRed-miR30-vgat2.shRNA

Use

AAV, Cre/Lox, Mouse Targeting, and RNAi

Expression

Mammalian

Promoter

h-synapsin

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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LXRE_Luc

Plasmid

#177622

Purpose

synthetic 3X LXRE promoter linked to luciferase in the pTAL vector

Depositor

Thomas Burris

Article

Griffett et al ACS Chem Biol. 2022 Apr 13. doi: 10.1021

Insert

3X LXRE

Use

Luciferase

Expression

Mammalian

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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Synapsin-cyto-iATPSnFR1.0

Plasmid

#102556

Purpose

expresses cytoplasmic ATP sensor in neurons

Depositor

Baljit Khakh

Article

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

iATPSnFR1.0

Use

AAV

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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PB-TET-mOct4

Plasmid

#20907

Purpose

piggyBac vector with tet inducible mOct4

Depositor

Andras Nagy

Article

Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

Insert

Oct4 (Pou5f1 Mouse)

Use

piggybac

Tags

IRES-bGeo

Expression

Mammalian

Available Since

April 23, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO hChR2(C128S/D156A)-EYFP

Plasmid

#35503

Purpose

AAV expression of Ef1a-driven, cre-dependent, stabilized step function opsin (SSFO) for optogenetic activation.

Depositor

Karl Deisseroth

Article

Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

Insert

hChR2(C128S/D156A)-EYFP

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

C128S and D156A

Promoter

Ef1a

Available Since

April 23, 2012

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_antiHer24D5-7_synNotch_Gal4VP64

Plasmid

#85424

Purpose

Lentiviral vector for expression of anti-Her2 scFv 24D5-7 Gal4VP64 synthetic Notch

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

anti-Her2_4D5-7_synNotch

Use

Lentiviral

Promoter

PGK

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCW57.1-Luciferase

Plasmid

#99283

Purpose

All-in-one lentivector with tet-inducible luciferase

Depositor

Stephen Tapscott

Article

Shadle et al PLoS Genet. 2017 Mar 8;13(3):e1006658. d

Insert

Firefly luciferase

Use

Lentiviral

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-EGFP-Kir4.1

Plasmid

#52874

Purpose

Express EGFP-Kir4.1 fusion protein in astrocytes

Depositor

Baljit Khakh

Article

Tong et al Nat Neurosci. 2014 May;17(5):694-703. do

Insert

Kir4.1 (Kcnj10 Rat)

Use

AAV; Adeno-associated virus ()

Expression

Mammalian

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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Synapsin-cyto-mRuby3-iATPSnFR1.0

Plasmid

#102557

Purpose

expresses cytoplasmic ATP sensor in neurons with red reference protein

Depositor

Baljit Khakh

Article

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

mRuby3-iATPSnFR1.0

Use

AAV

Tags

Ruby3

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pSR657

Plasmid

#65214

Purpose

Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-2 cap gene in Sf9 insect cells

Depositor

Robert Kotin

Article

Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

Inserts

modified AAV-2 rep78
modified AAV-2 cap

Use

AAV

Expression

Insect

Promoter

p10 and polyhedrin

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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AAV-U6-dgRNA-CAG-MPH

Plasmid

#106259

Purpose

Expresses MPH co-activator from the CAG promoter and one U6-driven dgRNA in AAV backbone

Depositor

Juan Belmonte

Article

Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

Insert

MS2-P65-HSF1 (HSF1 Synthetic, Human)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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