We narrowed to 5,844 results for: pCas

1. px330-UFM1 sgRNA2

   Plasmid

   #134634

   Purpose

   contains sgRNA targeting human UFM1 for gene knockout

   Depositor

   Yihong Ye

   Article

   Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

   Insert

   UFM1 sgRNA2 (UFM1 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. px330-UFM1 sgRNA1

   Plasmid

   #134633

   Purpose

   contains sgRNA targeting human UFM1 for gene knockout

   Depositor

   Yihong Ye

   Article

   Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

   Insert

   UFM1 sgRNA1 (UFM1 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEn-C1.1

   Plasmid

   #61479

   Purpose

   Encodes CRISPR sgRNA for Gateway or conventional cloning of 1-3 sgRNAs into pDe-CAS9 or pCAS9-TPC

   Depositor

   Holger Puchta

   Article

   Schiml et al Plant J. 2014 Oct 18. doi: 10.1111/tpj.1

   Insert

   AtU6-26:sgRNA
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   U6-26

   Available Since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. BPK1169: CAG-DmrC-NLS-3xFLAG-P65

   Plasmid

   #104564

   Purpose

   Mammalian expression vector for DmrC fused to p65 activation domain

   Depositor

   Keith Joung

   Article

   Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

   Insert

   DmrC-p65
   

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pC1300_pUB10_pcoCASphi_E9t_version2_U6_AtPDS3_gRNA10

   Plasmid

   #197952

   Purpose

   T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the C-terminal , and the AtPDS3 guide RNA 10 driven by U6 promoter.

   Depositor

   Steven Jacobsen

   Article

   Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

   Inserts

   pcoCasphi-2
   AtPDS3 gRNA10
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   AtU6-26 and pUBQ10

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. TDP43 NOTAG6

   Plasmid

   #28209

   Depositor

   Zuoshang Xu

   Article

   Yang et al PLoS One. 2010 . 5(12):e15878.

   Insert

   Tar DNA binding protein (TARDBP Human)
   

   Expression

   Mammalian

   Mutation

   aa1-314 only

   Available Since

   Sept. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pUC57-Amp_CRISPIE donor B3

   Plasmid

   #172844

   Purpose

   CRISPIE donor B3 (Zhong et al, eLife 2021), mTurquoise2 translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   CRISPIE designer exon (phase 0-0) encoding mTurquoise2
   

   Use

   CRISPR

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC57-Amp_CRISPIE donor B4

   Plasmid

   #172845

   Purpose

   CRISPIE donor B4 (Zhong et al, eLife 2021), mNeonGreen translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   CRISPIE designer exon (phase 0-0) encoding mNeonGreen
   

   Use

   CRISPR

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. px330-UFL1 sgRNA2

   Plasmid

   #134636

   Purpose

   contains sgRNA targeting human UFL1 for gene knockout

   Depositor

   Yihong Ye

   Article

   Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

   Insert

   UFL1 sgRNA2 (UFL1 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZeo-Cas9-gate

    Plasmid

    #113744

    Purpose

    Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving Zeocin for selection in plants.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    Cas9
    

    Use

    CRISPR; Gateway destination vector

    Promoter

    Z. Mays ubiquitin promoter, 35S promoter

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRvCAHS3-mEGFP

    Plasmid

    #205008

    Purpose

    The vector contains 1kbp of the upstream and downstream regions of the cahs3 gene from Ramazzottius varieornatus, with the mEGFP gene positioned in between.

    Depositor

    Kazuharu Arakawa

    Article

    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

    Insert

    mEGFP flanked by 1kbp upstream and downstream of the cahs3 gene from Ramazzottius varieornatus
    

    Use

    Tardigrade expression

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRvACT-mCherry

    Plasmid

    #205002

    Purpose

    The vector contains 1kbp of the upstream and downstream regions of the actin gene from Ramazzottius varieornatus, with the mCherry gene with NLS sequence positioned in between.

    Depositor

    Kazuharu Arakawa

    Article

    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

    Insert

    mCherry-NLS flanked by 1kbp upstream and downstream of the actin gene from R. varieornatus
    

    Use

    Tardigrade expression

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. px330-UFL1 sgRNA1

    Plasmid

    #134635

    Purpose

    contains sgRNA targeting human UFL1 for gene knockout

    Depositor

    Yihong Ye

    Article

    Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

    Insert

    UFL1 sgRNA1 (UFL1 Human)
    

    Expression

    Mammalian

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-ATG13

    Plasmid

    #171413

    Purpose

    For the purifcation of human ULK1 complex

    Depositor

    James Hurley

    Article

    Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

    Insert

    ATG13 (ATG13 Synthetic)
    

    Expression

    Mammalian

    Available Since

    July 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. TDP43 NOTAG3

    Plasmid

    #28208

    Depositor

    Zuoshang Xu

    Article

    Yang et al PLoS One. 2010 . 5(12):e15878.

    Insert

    Tar DNA binding protein (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    aa170-414 only

    Available Since

    Sept. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pJK01_Rho_minprox_DsREd

    Plasmid

    #173489

    Purpose

    PCR template for reporter gene with mouse Rhodopsin promoter with DsRed

    Depositor

    Barak Cohen

    Article

    White et al Proc Natl Acad Sci U S A. 2013 Jul 16;11

    Insert

    Mouse Rhodopsin promoter driving DsRed reporter gene
    

    Expression

    Mammalian

    Promoter

    Mouse Rhodopsin

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330-PITCh-TUBB4B

    Plasmid

    #207785

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of TUBB4B for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of TUBB4B (TUBB4B Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Double UP mNeon to mScarlet Rac1-N17

    Plasmid

    #125137

    Purpose

    Natively produces mNeonGreen, in the presence of Cre will be recombined to produce mScarlet and an untagged Rac1-N17 (Dominant Negative).

    Depositor

    Erik Dent

    Article

    Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d

    Insert

    mNeon/mScarletP2A Rac1-N17
    

    Expression

    Mammalian

    Mutation

    Removed Not1 site in mScarlet. 17th AA in Rac1 ch…

    Promoter

    CAG

    Available Since

    May 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. A3A-PBE-ΔUGI

    Plasmid

    #119770

    Purpose

    Conversion of C to T in wheat plants or monocotyledons protoplasts without uracil glycosylase inhibitor (UGI)

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

    Insert

    APOBEC3A-nCas9-ΔUGI-NLS
    

    Tags

    NLS

    Expression

    Plant

    Mutation

    nCas9 has a mutation D10A in wild type SpCas9

    Promoter

    maize Ubiquitin-1

    Available Since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. CP1028-ABE8e-dimer

    Plasmid

    #138498

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(WT)-ecTadA(8e)-nSpCas9 (CP1028)
    

    Expression

    Mammalian

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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