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pFRETtv-2in1-CN

Plasmid

#105119

Purpose

FRET/FLIM analysis in plants (stable or transient transformation)

Depositor

Christopher Grefen

Article

Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

Type

Empty backbone

Tags

mTRQ2 and mVenus

Expression

Plant

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pFRETtv-2in1-CC

Plasmid

#105116

Purpose

FRET/FLIM analysis in plants (stable or transient transformation)

Depositor

Christopher Grefen

Article

Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

Type

Empty backbone

Tags

mTRQ2/mVenus

Expression

Plant

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pHN1_lacUV5-mNG-PDT

Plasmid

#165583

Purpose

Expresses mNeonGreen and C-terminally fused PDT

Depositor

Daniel Ducat

Article

Sakkos et al ACS Synth Biol. 2021 Jul 16;10(7):1667-1

Insert

mNeonGreen-PDT

Tags

Protein degradation tag

Expression

Bacterial

Promoter

Ptrc

Available Since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pGC4593

Plasmid

#110206

Purpose

Accessory plasmid with inducible TEV protease and unnatural tRNA system (AcF)

Depositor

Adam Arkin, Guillaume Cambray

Article

Cambray et al Nat Biotechnol. 2018 Nov;36(10):1005-101

Inserts

TEV protease
aaRS

Expression

Bacterial

Promoter

Pbad and Ptet

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pUltra-FPheKRS

Plasmid

#136631

Purpose

Genetic Incorporation 4-fluorophenyl carbamate lysine (FPheK) into proteins

Depositor

Han Xiao

Article

Yu et al Bioconjug Chem. 2018 Nov 21;29(11):3522-

Insert

FPheKRS

Expression

Bacterial

Promoter

Tac1 promoter

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pBad33_ISAs26_reRNA

Plasmid

#205326

Purpose

Bacterial expression of ISAs26 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISAs26 TnpB

Expression

Bacterial

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pSB_syb25

Plasmid

#197992

Purpose

Expressed sybody 25 as a C-terminal myc-His-tagged construct

Depositor

Simon Newstead

Article

Parker et al Nat Struct Mol Biol. 2023 Jul 23. doi: 1

Insert

Sybody 25

Tags

Myc-His

Expression

Bacterial

Promoter

Arabinose

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAct-GW-HygroR

Plasmid

#149610

Purpose

Gateway compatible expression plasmid for Drosophila and establishing stable cell lines by Hygromycin selection. Actin5c ubiquitous promoter.

Depositor

Norbert Perrimon

Article

Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

Gateway cassette (ccdb + Chlor.R.)

Expression

Insect

Promoter

Act5c

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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PNCA-luciferase

Plasmid

#67793

Purpose

Use together with pCMV-intron and VSV-G to package MMLV reporter virus

Depositor

Stephen Goff

Article

Wang et al J Virol. 2015 Mar;89(5):2813-9. doi: 10.

Insert

MMLV-LTR-firefly luciferase

Expression

Mammalian

Available Since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only
pET28-his6-HA3-SNAPf

Plasmid

#188941

Purpose

T7 expression vector for making his6- and HA3-tagged SNAP protein

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

his6-HA3-SNAPf

Expression

Bacterial

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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Ec2Avs2

Plasmid

#188790

Purpose

Avs2 from Escherichia coli NCTC10650

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

Ec2Avs2

Expression

Bacterial

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAB168

Plasmid

#101671

Purpose

Opto-T7RNAP*(563-F1), equal expr.

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(563-F1), equal expr.

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pFRETtv-2in1-NN

Plasmid

#105125

Purpose

FRET/FLIM analysis in plants (stable or transient transformation)

Depositor

Christopher Grefen

Article

Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

Type

Empty backbone

Tags

mTRQ2/mVenus

Expression

Plant

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pPTK009-3a-αAmylase-αMFΔ

Plasmid

#84968

Purpose

Type 3a, α-Amylase followed by αMFΔ - secretion signal

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

α-Amylase followed by MFΔ secretion tag

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pSLICER

Plasmid

#197288

Purpose

Conjugative plasmid that facilitates seamless deletions in D. radiodurans following insertion of a seamless deletion cassette. Contains an origin of transfer

Depositor

Bogumil Karas

Article

Brumwell et al Biodes Res. 2023 Mar 15;5:0009. doi: 10.

Insert

I-SceI Endonuclease

Use

Synthetic Biology; Bacterial and yeast cloning

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pJB153

Plasmid

#92328

Purpose

Express mEos3.2 tagged ZapA in E coli

Depositor

Jie Xiao

Article

Lyu et al Biopolymers. 2016 Oct;105(10):725-34. do

Insert

ZapA

Tags

mEos3.2

Expression

Bacterial

Promoter

T5-lac

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pGT-Ah5

Plasmid

#122572

Purpose

MAGIC donor plasmid (Himar transposon with catP/sfGFP payload)

Depositor

Harris Wang

Article

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

catP/sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pPTK010-3a-Glucoamylase-αMFΔ

Plasmid

#84969

Purpose

Type 3a, Glucoamylase followed by αMFΔ - secretion signal

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Glucoamylase followed by αMFΔ secretion tag

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

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pSIBR4-Cre

Plasmid

#219435

Purpose

Theophylline_induced self_splicing intron_based riboswitch for the moderate expression of Cre recombinase

Depositor

Sheng Yang

Article

Xu et al Biotechnol Bioeng. 2024 Mar;121(3):1163-

Insert

intron 4-Cre

Expression

Bacterial

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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pKEB31

Plasmid

#31224

Depositor

Adam Bogdanove, Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

ccdB and Cm resistance gene

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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