We narrowed to 7,176 results for: cas9 plasmid

1. pMpGE_En01

   Plasmid

   #71534

   Purpose

   Used to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorpha

   Depositor

   Ikuko Hara-Nishimura, Takayuki Kohchi

   Article

   Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. tdTomato/pTREX-b

   Plasmid

   #68709

   Purpose

   Expresses tdTomato in pTREX-b vector which confers resistance to blasticidin. This vector is used for cloning a specific sgRNA by BamHI, to transfect Cas9-expressing Trypanosoma cruzi epimastigotes.

   Depositor

   Roberto Docampo

   Article

   Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

   Insert

   tdTomato
   

   Use

   Expression of tdtomato red fluorescence protein i…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSP2253

   Plasmid

   #70704

   Purpose

   Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Mutation

   E782K, N968K and R1015H in SaCas9

   Promoter

   T7

   Available since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. APq5210

   Plasmid

   #66086

   Purpose

   co-expression of Cas9 and a sgRNA targeting K08F4.2 in the middle of the ORF

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

   Insert

   sgRNA for APa4-2
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   U6

   Available since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

5. gh23

   Plasmid

   #106696

   Purpose

   expression of gRNA targeting POMT1, Cas9, and GFP

   Depositor

   Eric-Paul Bennett

   Article

   Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

   Insert

   POMT1 (POMT1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. AP303-4

   Plasmid

   #66088

   Purpose

   co-expression of Cas9 and a sgRNA targeting 5'end of C.elegans K08F4.2

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

   Insert

   sgRNA for APs1
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   U6

   Available since

   July 28, 2015

   Availability

   Academic Institutions and Nonprofits only

7. AP334-5

   Plasmid

   #66096

   Purpose

   co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

   Insert

   sgRNA for APs5
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   U6

   Available since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

8. APq5271

   Plasmid

   #66085

   Purpose

   co-expression of Cas9 and a sgRNA targeting K08F4.2 in the middle of the ORF

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

   Insert

   sgRNA for APa4-2
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   U6

   Available since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

9. APCSD 54

   Plasmid

   #66100

   Purpose

   co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans swan-2

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

   Insert

   sgRNA for CSD 54
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   U6

   Available since

   July 2, 2015

   Availability

   Academic Institutions and Nonprofits only

10. APCSD 53

    Plasmid

    #66099

    Purpose

    co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans swan-2

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

    Insert

    sgRNA for CSD 53
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available since

    July 2, 2015

    Availability

    Academic Institutions and Nonprofits only