We narrowed to 6,250 results for: nls

1. pLenti-VRERRA-P2A-GFP-PGK-Puro

   Plasmid

   #110865

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VRERRA-P2A-GFP in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VRERRA
   

   Use

   Lentiviral

   Tags

   FLAG

   Mutation

   D1135V, G1218R, R1335E, T1337R, and NLS sequence …

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-VRERRA-P2A-Puro

   Plasmid

   #110852

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VRERRA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VRERRA
   

   Use

   Lentiviral

   Tags

   FLAG

   Mutation

   D1135V, G1218R, R1335E, T1337R, and NLS sequence …

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSCV-NSD3-short del(100-263)

   Plasmid

   #72548

   Purpose

   expresses 3*FLAG tagged human NSD3-short with 100-263 aa deletion

   Depositor

   Christopher Vakoc

   Article

   Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

   Insert

   NSD3-short (NSD3 Human)
   

   Use

   Retroviral

   Tags

   3xFlag and SV40 NLS

   Expression

   Mammalian

   Mutation

   100-263 aa deletion

   Promoter

   LTR

   Available Since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEMS1210

   Plasmid

   #29107

   Purpose

   Expression of the eGFP reporter gene was not detected in the brain and eye.

   Depositor

   Elizabeth Simpson

   Article

   de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

   Insert

   Ple238 (TRH Human)
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   EGFP-NLS

   Available Since

   Nov. 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCIN4-EZOD

   Plasmid

   #44977

   Depositor

   Robert Oshima

   Article

   Galang et al J Biol Chem. 1996 Apr 5;271(14):7992-8.

   Insert

   Ets2 DBD (Ets2 Mouse)
   

   Tags

   FLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   Ets2 DNA binding domain (AA 332-468) only = domin…

   Promoter

   CMV

   Available Since

   May 28, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pEMS1136

   Plasmid

   #29056

   Purpose

   Expression of the eGFP reporter gene was not detected in the brain and eye.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   Ple198 (SLC6A4 Human)
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   EGFP-NLS

   Available Since

   Nov. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-PE SpG_ATH51

   Plasmid

   #242072

   Purpose

   Expresses SpG_ATH51 prime editor and pegRNA in mammalian cells

   Depositor

   Tobias Cantz

   Article

   Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

   Insert

   SpG_ATH51_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
   

   Use

   CRISPR and Synthetic Biology; Piggybac transposon

   Tags

   bpSV40 NLS

   Expression

   Mammalian

   Mutation

   ectopic insertion of 7 amino acids of PID turn-he…

   Available Since

   Oct. 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-PE iSM_PPL2

   Plasmid

   #235996

   Purpose

   Expresses iSM_PPL2 prime editor and pegRNA in mammalian cells

   Depositor

   Tobias Cantz

   Article

   Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

   Insert

   iSM_PPL2_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
   

   Use

   CRISPR and Synthetic Biology; Piggybac transposon

   Tags

   bpSV40 NLS

   Expression

   Mammalian

   Mutation

   replacement of 5 amino acids of Smac-Cas9 PL2 wit…

   Available Since

   Aug. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pC131: pAAV.CMV-CasRx-VEGFA sgRNA array

   Plasmid

   #203444

   Purpose

   Plasmid expressing active RfxCas13d with an array of three VEGFA mRNA targeting gRNAs

   Depositor

   Guei-Sheung Liu

   Article

   Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

   Inserts

   U6-VEGFA sgRNA array
   RfxCas13d
   

   Use

   AAV

   Tags

   HA and NLS

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pM155: pAAV.CMV-Cas13e (GenScript CO)-NT sgRNA

    Plasmid

    #203451

    Purpose

    Plasmid expressing active and codon optimised Cas13e with non-targeting gRNA

    Depositor

    Guei-Sheung Liu

    Article

    Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

    Inserts

    U6-non targeting (NT) sgRNA
    Cas13e
    

    Use

    AAV

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Codon optimised using GenScript tool

    Promoter

    CMV and U6

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pM156: pAAV.CMV-Cas13e (GenScript CO)-VEGFA sgRNA

    Plasmid

    #203452

    Purpose

    Plasmid expressing active and codon optimised Cas13e with VEGFA gRNA

    Depositor

    Guei-Sheung Liu

    Article

    Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

    Inserts

    U6-VEGFA sgRNA
    Cas13e
    

    Use

    AAV

    Tags

    HA and NLS

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. hA3A*-IscBn

    Plasmid

    #227146

    Purpose

    Vector encoding human codon-optimized nOgeuIscB fusion with two copies of UGI and hA3A* variant driven by CMV promoter

    Depositor

    Dali Li

    Article

    Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e

    Insert

    bpNLS-hA3A*-linker-nOgeuIscB-nucleoplasmin NLS-2XUGI-bpNLS
    

    Mutation

    D96R/E84R/V159R/H339A

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV-CMV-scFVDnmt3A_bGHpA

    Plasmid

    #177349

    Purpose

    AAV expression of scFV-fused catalytic domain of Dnmt3a for targeted DNA methylation editing

    Depositor

    Naoki Yamamoto

    Article

    Yamamoto et al Research Square [Preprint] 2021. doi: 10

    Insert

    catalytic domain of DNA Methyltransferase 3 Alpha (Dnmt3a Mouse)
    

    Use

    AAV

    Tags

    myc and scFV

    Expression

    Mammalian

    Mutation

    598–908 aa of mouse Dnmt3a

    Promoter

    CMV

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-TRE-scFVDnmt3A_bGHpA

    Plasmid

    #177355

    Purpose

    AAV expression of scFV-fused catalytic domain of Dnmt3a from doxycycine-inducible promoter for targeted DNA methylation editing

    Depositor

    Naoki Yamamoto

    Article

    Yamamoto et al Research Square [Preprint] 2021. doi: 10

    Insert

    catalytic domain of DNA Methyltransferase 3 Alpha (Dnmt3a Mouse)
    

    Use

    AAV

    Tags

    myc and scFV

    Expression

    Mammalian

    Mutation

    598–908 aa of mouse Dnmt3a

    Promoter

    Tetracycline-dependent promoter

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-TRE-scFVDnmt3L_bGHpA

    Plasmid

    #177356

    Purpose

    AAV expression of scFV-fused C terminal domain of Dnmt3l from doxycycine-inducible promoter for targeted DNA methylation editing

    Depositor

    Naoki Yamamoto

    Article

    Yamamoto et al Research Square [Preprint] 2021. doi: 10

    Insert

    C-terminal domain of mouse Dnmt3l (Dnmt3l Mouse)
    

    Use

    AAV

    Tags

    myc and scFV

    Expression

    Mammalian

    Mutation

    194–415 aa

    Promoter

    CMV promoter

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-TRE-scFVtet1_bGHpA

    Plasmid

    #177357

    Purpose

    AAV expression of scFV-fused catalytic domain of TET1 from doxycycine-inducible promoter for targeted DNA methylation editing

    Depositor

    Naoki Yamamoto

    Article

    Yamamoto et al Research Square [Preprint] 2021. doi: 10

    Insert

    Tet Methylcytosine Dioxygenase 1 (TET1 Human)
    

    Use

    AAV

    Tags

    myc and scFV

    Expression

    Mammalian

    Mutation

    N terminal domain of human Tet1

    Promoter

    Tetracycline-dependent promoter

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIRHD-TetR-GAL80-Rho1-GAL4

    Plasmid

    #218274

    Purpose

    Introduction of low-temperature-inducible GAL system, LowTempGAL v2.2

    Depositor

    Christopher Howard

    Article

    Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

    Insert

    gal80Arm1-pAgTEF1>KanMX4>tAgTEF1-pHAC1>UBI4>>DHFRP66L-TetR>NLS>TUP1>tABF1-pTEF2>Rho1C17R>>GAL4>tGAL4-pTEF1(TetO)>GAL80Arm2
    

    Expression

    Yeast

    Mutation

    DHFR (P66L), Rho1 (C17R)

    Available Since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. B-HypaR-SpCas9

    Plasmid

    #126764

    Purpose

    Expression plasmid for human codon-opt. increased fidelity Blackjack-Hypa2SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than Hypa2SpCas9.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-Hypa2SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    R661A; N692A; M694A; Q695A; H698A; amino acids 10…

    Promoter

    CBh

    Available Since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEJS1831 All-in-one AAV-U1a-NmeABE-8e-2xBPSV40-miniU6-Fah

    Plasmid

    #199264

    Purpose

    Single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one miniU6 driven sgRNA targeting the point mutation in the mouse Fah gene of a HT1 mouse model

    Depositor

    Erik Sontheimer

    Article

    Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.

    Insert

    NmeABE8e
    

    Use

    AAV and CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Available Since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAT079

    Plasmid

    #180509

    Purpose

    Plasmid expressing mammalian codon optimized wt DpbCasX, sgRNAv2 scaffold, and restriction sites to clone in new spacers

    Depositor

    Jennifer Doudna

    Article

    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

    Inserts

    CasX sgRNAv2
    DpbCasX
    

    Use

    CRISPR

    Tags

    2x FLAG and SV40 NLS

    Expression

    Mammalian

    Promoter

    CAG and U6

    Available Since

    June 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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