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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 21,078 results for: nls

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Showing: 4921 - 4940 of 21078 results

  9. pSmart-EF1a-Nla-cas3

    Plasmid
    #178881
    Purpose
    Expression of human codon-optimized NlaCas3 with C terminal NLS and HA tag
    Depositor
    Yan Zhang
    Publication
    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EF1a-NlaCas3c-bGH polyA
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Jan. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    178881-plasmid-map-sequence-id-351754

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  10. pLenti EF1a mCherry-T2A-VP64-NtcA-2xNLS_SV40

    Plasmid
    #254310
    Purpose
    Constitutive expression of VP64-NtcA-2xNLS_SV40 for use in the functional aKG-ON biosensor. Requires pLenti Neo glnA PCC 6803 reporter gene plasmid. mCherry to monitor NtcA chimera expression.
    Depositor
    Samuel McBrayer
    Publication
    Sternisha et al Science. 2026 Jul 16;393(6808):eadx8675.
    Insert
    VP64-NtcA-2xNLS_SV40
    Use
    Lentiviral and Synthetic Biology
    Tags
    FLAG and FLAG-mCherry-T2A
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    July 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    254310-plasmid-map-sequence-id-508529

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  12. pv6_BRD4(2)_BRD.2x

    Plasmid
    #260597
    Purpose
    Expresses two copies of the second bromodomain from BAZ2a fused to eGFP including flexible linker and NLS
    Depositor
    Tuncay Baubec
    Insert
    BRD4(2)_BRD.2x (Brd4 Mouse)
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    CAGGS
    Available since
    Oct. 16, 2026
    Availability
    Academic Institutions and Nonprofits only
    260597-plasmid-map-sequence-id-523811

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  13. pCR197

    Plasmid
    #240643
    Purpose
    Expression of the tri-cistronic construct NES-AmNeonGreen-P2A-mTurquoise2-NLS-T2A-mScarlet-I-PTS1 in Exaiptasia diaphana; In vitro transcription of the same construct via SP6 polymerase
    Depositor
    John Pringle
    Inserts
    AIPGENE865 promoter
    SP6 promoter
    AmNeonGreen
    mTurquoise2
    mScarlet-I
    Use
    Gene expression and genomic integration in fish
    Tags
    NES (from Exaiptasia diaphana MEK2), NLS (from SV…
    Mutation
    Codon-optimized for Exaiptasia diaphana, amino ac…
    Available since
    Aug. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    240643-plasmid-map-sequence-id-482910

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  15. pDRM166 LKB (Luciferase mKate2 Blast)

    Plasmid
    #183502
    Purpose
    Expresses the firefly luciferase gene, the NLS-tagged mKate2 gene, and the blasticidin resistance gene joined by P2A linkers.
    Depositor
    Wayne Tilley
    Publication
    Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413
    Inserts
    Firefly Luciferase
    mKate2
    BSD
    Use
    Lentiviral and Luciferase
    Tags
    P2A linker and SV40 NLS with GGS linker
    Expression
    Mammalian
    Promoter
    MND, MND (off Luc-P2A), and MND (off Luc-P2A-mKat…
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    183502-plasmid-map-sequence-id-362519

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  17. MSP2262

    Plasmid
    #70703
    Purpose
    Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 1: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus dCas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    D10A & H557A in SaCas9
    Promoter
    T7
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    70703-plasmid-map-sequence-id-127765

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  18. mPlum-PCNA-19-SV40NLS-4

    Plasmid
    #55994
    Purpose
    Localization: Nucleus, Excitation: 590, Emission: 649
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PCNA-19 SV40NLS (PCNA Human)
    Tags
    mPlum
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only

  19. mCitrine-PCNA-19-SV40NLS-4

    Plasmid
    #56564
    Purpose
    Localization: Nucleus, Excitation: 516, Emission: 529
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PCNA-19 SV40NLS (PCNA Human)
    Tags
    mCitrine
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 5, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by

  20. GPKAnls

    Plasmid
    #61094
    Purpose
    GFP-tagged PKA catalytic subunit targeted to the nucleus by addition of a nuclear localization signal
    Depositor
    Steven Green
    Publication
    Bok et al J Neurosci. 2003 Feb 1;23(3):777-87.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pkac2
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 4921 - 4940 of 21078 results