We narrowed to 9,888 results for: Coli
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Plasmid#122450PurposeExpression of inositol 1,4,5-trisphosphate 3-kinase ADepositorInsertinositol 1,4,5-trisphosphate 3-kinase A (Itpka Rat)
Tagsbeta-galactosidaseExpressionBacterialMutationcoding sequence from V66 till stop codonAvailable SinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
N295A (EcN), E166Q (EcN) MetNI LmH
Plasmid#118269PurposeN295A and E166Q Mutation of MetN in Methionine ABC Transporter with 6x His Tag. Used to solve crystal structureDepositorTags6x His TagExpressionBacterialMutationChanged Glutamic Acid (Glu) 166 to Glutamine (Gln…PromoterT7 PromoterAvailable SinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-Nterm 6xHis PL K16TAG-T500
Plasmid#92315PurposeExpression of NTerm 6xHis tagged protein L (PL) mutant K16TAG for ncAA incoporation using the p70a promoter and UTR1DepositorInsertB1 domain of protein L (PL) mutant K16TAG
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialPromoterp70b (p70a promoter with one mutation)Available SinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-deGFP Y35TAG D193TAG-6xHis-T500
Plasmid#92227PurposeExpression of deGFP mutant Y35TAG D193TAG for ncAA incoporation using the p70a promoter and UTR1DepositorInsertdeGFP Y35TAG D193TAG
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationResidues Y35 D193 were mutated into TAG (Amber) s…Promoterp70aAvailable SinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR3-Nterm 6xHis mRFP K15TAG-T500
Plasmid#92319PurposeExpression of NTerm 6xHis tagged mRFP mutant K15TAG for ncAA incoporation using the p70a promoter and UTR3DepositorInsertOptimized NTerm 6xHis tagged monomeric RFP (mRFP)
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialPromoterp70aAvailable SinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-Nterm 6xHis mRFP K15TAG-T500
Plasmid#92318PurposeExpression of NTerm 6xHis tagged mRFP mutant K15TAG for ncAA incoporation using the p70a promoter and UTR1DepositorInsertOptimized NTerm 6xHis tagged monomeric RFP (mRFP)
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialPromoterp70aAvailable SinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70b-UTR1-NTerm 6xHis-zmADH-T500
Plasmid#92229PurposeExpression of zmADH using the p70b (weak version of the p70a promoter) promoter and UTR1DepositorInsertNTerm 6xHis Tagged zmADH
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialPromoterp70b (p70a promoter with one mutation)Available SinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-NTerm 6xHis-zmADH V86TAG-T500
Plasmid#92230PurposeExpression of zmADH mutant V86TAG for ncAA incoporation using the p70a promoter and UTR1DepositorInsertNTerm 6xHis Tagged zmADH
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationResidue V86 mutated into TAG (Amber) stop codon f…Promoterp70aAvailable SinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZS1[GalS-T]
Plasmid#60753PurposeContains PIq driving expression GalS-T, the Fucose inducible chimera with the TAN DBD.DepositorInsertsGalS-T
GalS-T
UseSynthetic BiologyExpressionBacterialMutationChimeric LacI/GalR repressor with the TAN DBD and…Available SinceJuly 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pHyperTEV60
Plasmid#228496Purpose10xHis-SUMO (Smt3) tagged HyperTEV60 engineered protease (E. coli based protein expression)DepositorInsertTEV Protease
Tags10xHis-SUMO(Smt3)ExpressionBacterialPromoterT7 PromoterAvailable SinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSm-Nuclease
Plasmid#132431PurposeExpresses Serratia marcescens nuclease in E. coli using T7 promoterDepositorInsertSerratia marcescens nuclease (codon optimized)
TagsNoneExpressionBacterialPromoterT7Available SinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOpen-DpnIR
Plasmid#165529PurposeUsed to digest methylated DNA; to be expressed in dam- E. coli strains (strains which do not methylate their genomic DNA)DepositorInsertDpnI
UseSynthetic BiologyAvailable SinceAug. 5, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTRC-LIC
Plasmid#62343PurposeUsed for tac promoter regulated high-level expression of cloned, untagged genes in E. Coli.DepositorTypeEmpty backboneExpressionBacterialPromotertac promoter from pKK233-2Available SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL
Plasmid#62304PurposeIt is a donor vector for generation of recombinant baculovirus by site-specific transposition in an E. coli host.DepositorTypeEmpty backboneUseBaculovirus expressionTags6xHis and TEV cleavage sitePromoterpolyhedrinAvailable SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
YopH-pNIC-Bsa4
Plasmid#92063PurposepNIC expression vector encoding gene for YopHDepositorInsertYopH
Tags6x His TagExpressionBacterialMutationCodon optimized E. coliAvailable SinceJan. 17, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
p28BIOH-LIC
Plasmid#62352PurposeE. coli expression vector with N-terminal AviTag and a C-terminal Hexa-HIS tagDepositorTypeEmpty backboneTags6xHis and AviTagExpressionBacterialPromoterT7-lacOAvailable SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMalAnPL
Plasmid#67281PurposeExpresses MBP-Anacystis nidulans photolyase in E. coliDepositorInsertphotolyase Anacystis nidulans
Tagsmaltose binding proteinExpressionBacterialMutationnone knownAvailable SinceJuly 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1-NDFIP2
Plasmid#179330PurposeE. coli optimized expression of GST-fusion of human NDFIP2DepositorAvailable SinceApril 7, 2022AvailabilityAcademic Institutions and Nonprofits only