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pHAGE-IRES-puro-NLS-dHgm6Cas13b-EGFP-NLS

Plasmid

#191366

Purpose

Overexpression of NLS-dHgm6Cas13b-EGFP-NLS in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-dHgm6Cas13b-EGFP-NLS

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dBba2Cas13b-EGFP-NLS

Plasmid

#191362

Purpose

Overexpression of NLS-dBba2Cas13b-EGFP-NLS in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-dBba2Cas13b-EGFP-NLS

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPba3Cas13b-EGFP-NLS

Plasmid

#191361

Purpose

Overexpression of NLS-dPba3Cas13b-EGFP-NLS in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-dPba3Cas13b-EGFP-NLS

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR/Zeo-sfGFP-SKL

Plasmid

#186720

Purpose

Gateway entry vector encoding sfGFP with the two peroxisome-targeting signals (out of frame).

Depositor

Yutaka Kodama

Article

Konno et al Biochem Biophys Res Commun. 2018 Sep 3;5

Insert

sfGFP-SKL

Expression

Bacterial

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_113

Plasmid

#180540

Purpose

Entry vector containing Signal peptide region of CsgA including SecB-N22 sequenced

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

Signal peptide region of CsgA including SecB-N22 sequenced

Expression

Bacterial

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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H2B-MiniE-mEm

Plasmid

#181991

Purpose

MiniE genetically encoded tag on H2B-mEmerald to be used with MiniR labeled with a reporter

Depositor

Kimberly Beatty

Article

Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

Insert

H2B-MiniE-mEm

Tags

mEmerald

Expression

Mammalian

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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MPC027: pAAV-CAG-FLEX-sNovArch-PP-Citrine_KV2.1TS-ER2 (soma targeting)

Plasmid

#153196

Purpose

encodes pAAV soma targeted NovArch

Depositor

Adam Cohen

Article

Chien et al Sci Adv. 2021 May 5;7(19). pii: 7/19/eab

Insert

sNovArch

Use

AAV

Tags

citrine

Expression

Mammalian

Available Since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b(CoV1h)

Plasmid

#162822

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF3b(CoV1h)

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_ORF3b(CoV1h)

Use

Piggybac

Expression

Mammalian

Mutation

CoV2_ORF3b(CoV1h)(Met1,Amber)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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25Q-ymsfGFP

Plasmid

#128559

Purpose

Httex1 (without proline-rich region) fused to ymsfGFP

Depositor

Patrick Lajoie

Article

Albakri et al F1000Res. 2018 Aug 10;7:1242. doi: 10.12

Insert

25Q-Httex1 (without proline-rich region) fused to ymsfGFP

Tags

ymsfGFP

Expression

Yeast

Promoter

Gal1

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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68Q-ymScarlet

Plasmid

#128562

Purpose

Httex1 (without proline-rich region) fused to ymScarlet

Depositor

Patrick Lajoie

Article

Albakri et al F1000Res. 2018 Aug 10;7:1242. doi: 10.12

Insert

68Q-Httex1 (without proline-rich region) fused to ymSarlet

Tags

ymScarlet

Expression

Yeast

Promoter

Gal1

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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25Q-ymScarlet

Plasmid

#128561

Purpose

Httex1 (without proline-rich region) fused to ymScarlet

Depositor

Patrick Lajoie

Article

Albakri et al F1000Res. 2018 Aug 10;7:1242. doi: 10.12

Insert

25Q-Httex1 (without proline-rich region) fused to ymSarlet

Tags

ymScarlet

Expression

Yeast

Promoter

GAL1

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCNL-N1_LAMP1

Plasmid

#65685

Purpose

Expresses LAMP1-CNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

LAMP1

Use

Luciferase

Tags

CNL

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 15, 2016

Availability

Academic Institutions and Nonprofits only
pYNL-N1_LAMP1

Plasmid

#65684

Purpose

Expresses LAMP1-YNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

LAMP1

Use

Luciferase

Tags

YNL

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 15, 2016

Availability

Academic Institutions and Nonprofits only
pMZ036

Plasmid

#66973

Purpose

Expresses zebrafish Chk1

Depositor

Michael Lampson

Article

Zhang et al Cell Cycle. 2014;13(24):3828-38. doi: 10

Insert

zebrafish Chk1

Use

Zebrafish mrna injection

Expression

Mammalian

Mutation

Q341H mutation compared to GenBank reference sequ…

Promoter

CMV

Available Since

Jan. 13, 2016

Availability

Academic Institutions and Nonprofits only

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dEos-Vinculin-14

Plasmid

#57568

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Betzig et al Science. 2006 Sep 15;313(5793):1642-5. E

Insert

Vinculin (VCL Human)

Tags

dEos

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pLKO.1/CFP sh-hUBE2N

Plasmid

#246752

Purpose

Mammalian and lentiviral expression of shRNA targeting human UBE2N (Ubc13)

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2025 Aug 25. doi: 10.1038

Insert

sh-hUBE2N (UBE2N Human)

Use

Lentiviral and RNAi

Promoter

U6

Available Since

May 21, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19-HYG-BES1-AnTat1.1-telo

Plasmid

#245103

Purpose

Inserts VSG AnTat1.1 in T. brucei cells at Lister427 BES1

Depositor

Monica Mugnier

Article

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

AnTat1.1

Use

Other

Mutation

silent G to A mutation at position 471 within AnT…

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pMEF2c-AHF-Dre-nls (JDW 35)

Plasmid

#242585

Purpose

The Mef2c anterior heart field promoter driving expression of Dre recombinase.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

DRE-nls

Expression

Mammalian

Promoter

mouse Mef2c

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pET24a-VHH-TagRFP657

Plasmid

#243767

Purpose

Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to TagRFP657. VHH-TagRFP657 also contains a T7, HA, BAP and His6 epitope

Depositor

Dominik Buser, Martin Spiess

Article

Buser et al J Vis Exp. 2025 Oct 17;(224). doi: 10.37

Insert

anti-GFP nanobody VHH

Tags

T7-TEV site and TagRFP657-HA-AviTag-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pET24a-VHH-mNeptune2.5

Plasmid

#243765

Purpose

Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mNeptune2.5. VHH-mNeptune2.5 also contains a T7, HA, BAP and His6 epitope

Depositor

Dominik Buser, Martin Spiess

Article

Buser et al J Vis Exp. 2025 Oct 17;(224). doi: 10.37

Insert

anti-GFP nanobody VHH

Tags

T7-TEV site and mNeptune2.5-HA-AviTag-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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