We narrowed to 8,957 results for: plat

1. AICSDP-50: NPM1-mEGFP

   Plasmid

   #109122

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human NPM1

   Depositor

   Allen Institute for Cell Science

   Insert

   NPM1 Homology Arms with linker-mEGFP (NPM1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   May 4, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pBHM2404

   Plasmid

   #173441

   Purpose

   pRS316-mNeonGreen-CBP-2XFLAG-NAT1, contains C. neoformans optimized mNeonGreen and NAT1 marker for use as PCR template

   Depositor

   Hiten Madhani

   Article

   Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

   Insert

   mNeonGreen-CBP-2XFLAG-NAT1
   

   Tags

   CBP-2xFLAG

   Expression

   Bacterial and Yeast

   Available Since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. AICSDP-28:TUBA1B-mTagRFP-T

   Plasmid

   #101785

   Purpose

   Homology arms and mTagRFPT-linker sequence for N-terminus (second exon) tagging of human TUBA1B

   Depositor

   Allen Institute for Cell Science

   Insert

   TUBA1B Homology Arms with mTagRFPT-linker (TUBA1B Human)
   

   Use

   CRISPR; Donor template

   Tags

   mTagRFPT-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   Oct. 31, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pBHM2406

   Plasmid

   #173442

   Purpose

   pRS316-mNeonGreen-CBP-2XFLAG-HYGB, contains C. neoformans optimized mNeonGreen and HYG marker for use as PCR template

   Depositor

   Hiten Madhani

   Article

   Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

   Insert

   mNeonGreen-CBP-2XFLAG-HYGB
   

   Tags

   CBP-2xFLAG

   Expression

   Bacterial and Yeast

   Available Since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. AICSDP-22:CETN2-mTagRFP-T

   Plasmid

   #101781

   Purpose

   Homology arms and mTagRFPT-linker sequence for N-terminus tagging of human CETN2

   Depositor

   Allen Institute for Cell Science

   Insert

   CETN2 Homology Arms with mTagRFPT-linker (CETN2 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mTagRFPT-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   Oct. 31, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pT7-ZoAl-RTD-3xF-ORF

   Plasmid

   #238486

   Purpose

   For production of C-term flag-tagged ZoAl RT-dead ORF mRNA for mRNA transfection in PRINT

   Depositor

   Kathleen Collins

   Article

   Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

   Insert

   R2 retroelement mRNA
   

   Use

   Ivt template

   Tags

   3x-Flag

   Mutation

   DD644/645->AA

   Available Since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT7-ZoAl-END-3xF-ORF

   Plasmid

   #238485

   Purpose

   For production of C-term flag-tagged ZoAl EN-dead ORF mRNA for mRNA transfection in PRINT

   Depositor

   Kathleen Collins

   Article

   Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

   Insert

   R2 retroelement mRNA
   

   Use

   Ivt template

   Tags

   3x-Flag

   Mutation

   DD1041/1054->AA

   Available Since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT7-TaGu-RTD-3xF-ORF

   Plasmid

   #238490

   Purpose

   For production of C-term flag-tagged TaGu RT-dead ORF mRNA for mRNA transfection in PRINT

   Depositor

   Kathleen Collins

   Article

   Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

   Insert

   R2 retroelement mRNA
   

   Use

   Ivt template

   Tags

   3x-Flag

   Mutation

   DD660/661->AA

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT7-TaGu-END-3xF-ORF

   Plasmid

   #238489

   Purpose

   For production of C-term flag-tagged TaGu EN-dead ORF mRNA for mRNA transfection in PRINT

   Depositor

   Kathleen Collins

   Article

   Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

   Insert

   R2 retroelement mRNA
   

   Use

   Ivt template

   Tags

   3x-Flag

   Mutation

   DD1057/1070->AA

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. AICSDP-27:SLC25A17-mEGFP

    Plasmid

    #101784

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human SLC25A17

    Depositor

    Allen Institute for Cell Science

    Insert

    SLC25A17 Homology Arms with linker-mEGFP (SLC25A17 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    Oct. 31, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pMD19-PBBa_J23117-sgRNA-Spr

    Plasmid

    #190793

    Purpose

    Template vector to amplify single sgRNAs or pieces for multiplexing arrays. Has flanking BsaI-sites.

    Depositor

    Paul Hudson

    Article

    Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

    Insert

    sgRNA (dummy)
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    BBa_J23117

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMD19-Cas9_handle-S.pyogenes_terminator-PBBa_J23117-Cmr

    Plasmid

    #190794

    Purpose

    Template vector to amplify pieces for creating multiplex sgRNA-arrays.

    Depositor

    Paul Hudson

    Article

    Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

    Insert

    sgRNA-scaffold only
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    BBa_J23117

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHis-AID*-9myc

    Plasmid

    #99524

    Purpose

    C-terminal AID*-9myc degron cassette with HIS3MX marker for S. cerevisiae

    Depositor

    Helle Ulrich

    Article

    Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

    Insert

    IAA17(71-116) (AXR3 Mustard Weed)
    

    Use

    Pcr template for yeast tagging cassette

    Tags

    9xMyc

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. SpCas9-CBE6a-V106W

    Plasmid

    #215824

    Purpose

    Express CBE6a-V106W (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

    Insert

    CBE6a V106W-SpCas9-2xUGI (cas9 )
    

    Use

    In vitro transcription template

    Available Since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSUB11

    Plasmid

    #169699

    Purpose

    Template plasmid for generating C-terminal 3xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.

    Depositor

    Nara Figueroa

    Article

    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98

    Insert

    3xFLAG tag adjacent to FRT-flanked aph (KanR) gene
    

    Tags

    3xFLAG tag

    Expression

    Bacterial

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHyg-AID*-9myc

    Plasmid

    #99518

    Purpose

    C-terminal AID*-9myc degron cassette with HygR marker for S. cerevisiae

    Depositor

    Helle Ulrich

    Article

    Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

    Insert

    IAA17(71-116) (AXR3 Mustard Weed)
    

    Use

    Pcr template for yeast tagging cassette

    Tags

    9xMyc

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. AICSDP-72:TFAM-mEGFP

    Plasmid

    #159745

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human TFAM

    Depositor

    Allen Institute for Cell Science

    Insert

    TFAM Homology Arms with linker-mEGFP (TFAM Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    Oct. 13, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pCAGGS-mEGFP-loxP-P2A-dTKneo-pA-loxP

    Plasmid

    #139484

    Purpose

    PCR template for mEGFP gene knock-in donor vector

    Depositor

    Kazuhiro Aoki

    Article

    Komatsubara et al J Biol Chem. 2019 Apr 12;294(15):6062-60

    Insert

    mEGFP-loxP-P2A-dTKneo-pA-loxP
    

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. Brp-T2A-QF2w-Hsp70-3XP3-dsRed

    Plasmid

    #141094

    Purpose

    Template plasmid for inserting QF2w into the Brp gene of Aedes aegypti with CRISPR/Cas9, to generate a pan-neuronal driver line.

    Depositor

    Carolyn McBride

    Article

    Zhao et al Cell Rep Methods. 2021 Jul 26;1(3):10004

    Insert

    bruchpilot
    

    Use

    CRISPR

    Expression

    Insect

    Mutation

    coding sequence downstream of CRISPR cut site, sy…

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA-Flag-Kdm6b(1025-End)-pA

    Plasmid

    #100278

    Purpose

    In vitro transcription template (T7 promoter) for Kdm6b catalytic domain active form.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nature. 2017 Jul 19. doi: 10.1038/nature

    Insert

    Kdm6b (Kdm6b Mouse)
    

    Tags

    Flag epitope tag

    Expression

    Mammalian

    Mutation

    catalytic domain (1025aa-End)

    Promoter

    T7

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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