We narrowed to 7,024 results for: 11

1. pAAV.CAG-FLEX.SF-iGluSnFR.A184V.mRuby3

   Plasmid

   #106205

   Purpose

   Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Insert

   SF-iGluSnFR.A184V
   

   Use

   AAV

   Tags

   Expression

   Mutation

   GltI: A184V + mRuby3 fusion

   Promoter

   CAG

   Available since

   Feb. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-EF1α1.1-FLPX-rc [Chronos-GFP]

   Plasmid

   #122102

   Purpose

   AAV-mediated expression of Chronos-GFP under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a（1.1kb short version)

   Available since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-EF1α1.1-GFP

   Plasmid

   #122100

   Purpose

   AAV-mediated expression of GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   GFP
   

   Use

   AAV

   Tags

   NA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a（1.1kb short version)

   Available since

   May 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMOS010: pLX304-CheRiff-GFP

   Plasmid

   #62986

   Purpose

   Lentivirus production of CheRiff channelrhodopsin actuator

   Depositor

   Adam Cohen

   Article

   Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

   Insert

   CheRiff-eGFP
   

   Use

   Lentiviral; Gateway destination vector

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pD2_miniluc_newP7

   Plasmid

   #174105

   Purpose

   adding the mini luciferase and mini promoter to the MPRA lenti construct

   Depositor

   Paul Khavari

   Article

   Guo et al Nat Genet. 2023 Nov;55(11):1876-1891. do

   Insert

   mini luciferase mini-promoter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. MBP-rPKM2 WT

   Plasmid

   #107158

   Purpose

   MBP-rPKM2 WT for production and purification of rat PKM2 WT

   Depositor

   Jesse Rinehart

   Article

   Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

   Insert

   MBP-tagged rat pyruvate kinase M2 isoform (Pkm Rat)
   

   Use

   Tags

   C-term 6-his and N-term MBP

   Expression

   Bacterial

   Mutation

   Promoter

   PBAD

   Available since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-N3/hArf6(WT)-EGFP

   Plasmid

   #79423

   Purpose

   Expresses C-terminally EGFP-tagged Arf6(WT) in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

   Insert

   Arf6 (ARF6 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. STARR-seq luciferase INF enhancer vector_ORI_ISG15

   Plasmid

   #99322

   Purpose

   Luciferase validation vector with ISG15 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

   Depositor

   Alexander Stark

   Article

   Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

   Insert

   enhancer; chr1: 947976 -949995 (ISG15 Human)
   

   Use

   Luciferase; Starr-seq luciferase validation vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.CAG-FLEX.SF-iGluSnFR.S72A.mRuby3

   Plasmid

   #106206

   Purpose

   Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Insert

   SF-iGluSnFR.S72A
   

   Use

   AAV

   Tags

   Expression

   Mutation

   GltI: S72A + mRuby3 fusion

   Promoter

   CAG

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-CAG-FLPX-rc [Chronos-tdTomato]

    Plasmid

    #105834

    Purpose

    AAV-mediated expression of Chronos-tdTomato under the CAG promoter in frt/reversed (Flp-dependent) manner.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-tdTomato
    

    Use

    AAV

    Tags

    tdTomato (codon diversified version)

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLXSN p200 CUX1

    Plasmid

    #90470

    Purpose

    Retroviral vector expressing human p200 CUX1 with a Myc and HA tag at the N- and C-terminus, respectively

    Depositor

    Alain Nepveu

    Article

    Kedinger et al J Biol Chem. 2009 Oct 2;284(40):27701-11

    Insert

    CUX1 (CUX1 Human)
    

    Use

    Retroviral

    Tags

    Ha and myc

    Expression

    Mammalian

    Mutation

    Promoter

    Moloney murine leukemia virus long terminal repeat

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. lentiCRISPR v2-Nr4a1 double gRNAs

    Plasmid

    #162791

    Purpose

    Multiplex Guide RNAs to generate Nr4a1 knockout by CRISPR

    Depositor

    Matthew Steinhauser

    Article

    Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

    Insert

    Multiplex Nr4a1 gRNA 1 and 2 (Nr4a1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV.CAG.SF-Venus-iGluSnFR.A184V

    Plasmid

    #106202

    Purpose

    Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-Venus-iGluSnFR.A184V
    

    Use

    AAV

    Tags

    Expression

    Mutation

    SFGFP: T203Y, F46L, T65G, S72A GltI: A184V

    Promoter

    CAG

    Available since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEN6-Ubc5 Sc

    Plasmid

    #83234

    Purpose

    pHis-par2-UbHs/Ubc5Sc/ Uba1Ta

    Depositor

    Gali Prag

    Article

    Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

    Inserts

    Ubiqutin (UBC Human)
    Ubc 5 (UBC5 Budding Yeast)
    Uba 1
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-EF1α-FLEX-rc[Chronos-GFP]

    Plasmid

    #62725

    Purpose

    AAV-mediated expression of Chronos-GFP under the EF1α promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    April 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pINSPECT:IL2_SigP:NLuc

    Plasmid

    #197266

    Purpose

    Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human IL2 locus (exon 3). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

    Insert

    Interleukin-2 homology arms
    

    Use

    CRISPR, Luciferase, Synthetic Biology, and Unspec…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-hAsCas12a-RR(S542R/K607R)-NLS(nucleoplasmin)-3xHA (AAS1081)

    Plasmid

    #114094

    Purpose

    CAG promoter expression plasmid for human codon optimized AsCas12a-RR(S542R/K607R) nuclease with C-terminal NLS and HA tag

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized AsCas12a-RR (S542R/K607R)
    

    Use

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Mutation

    S542R and K607R

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. Fgf4enhLV (FpG12)

    Plasmid

    #69444

    Purpose

    positive control plasmid for lentiviral reporter expression in ESCs

    Depositor

    Lisa Dailey

    Article

    Murtha et al Nat Methods. 2014 May;11(5):559-65. doi:

    Inserts

    minimal FGF4 promoter
    EGFP
    Ubiquitin promoter
    Hygromycin resistance
    FGF4 enhancer (Fgf4 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV.CAG.SF-Venus-iGluSnFR.S72A

    Plasmid

    #106203

    Purpose

    Weak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-Venus-iGluSnFR.S72A
    

    Use

    AAV

    Tags

    Expression

    Mutation

    SFGFP: T203Y, F46L, T65G, S72A GltI: S72A

    Promoter

    CAG

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.A184S

    Plasmid

    #106183

    Purpose

    Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Has Service

    AAV1

    Insert

    SF-Venus-iGluSnFR.A184S
    

    Use

    AAV

    Tags

    Expression

    Mutation

    SFGFP: T203Y, F46L, T65G, S72A GltI: A184S

    Promoter

    hSynapsin

    Available since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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