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We narrowed to 21,078 results for: nls

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Showing: 4961 - 4980 of 21078 results

  8. AAV-TRE-dSaCas9-NLS-3xHA-3xSunTag_bGHpA

    Plasmid
    #177344
    Purpose
    AAV expression of a catalytically inactive SaCas9 (dSaCas9) fused with three copies of SunTag sequence from doxycycine-inducible promoter
    Depositor
    Naoki Yamamoto
    Publication
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Insert
    dead hSaCas9
    Use
    AAV and CRISPR
    Tags
    3x GCN4 peptide, 3xHA, and NLS
    Expression
    Mammalian
    Mutation
    D10A, N580A
    Promoter
    Tetracycline-dependent promoters
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177344-plasmid-map-sequence-id-353629

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  10. pMIIS1011

    Plasmid
    #238212
    Purpose
    Expresses ParTasR with an N-terminal HA-NLS tag under CMV promoter in human cells.
    Depositor
    Feng Zhang
    Publication
    Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ParTasR
    Tags
    HA-NLS
    Expression
    Mammalian
    Available since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    238212-plasmid-map-sequence-id-471745

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  11. pLH_Scr15

    Plasmid
    #100537
    Purpose
    CEN/ARS plasmid with expression cassettes for PhyBNT-CreN and PIF3-NLS-CreC fusion proteins for red/far-red light-regulated Cre recombinase activity in Saccharomyces cerevisiae.
    Depositor
    Bernd Müller-Röber
    Publication
    Hochrein et al Nat Commun. 2018 May 22;9(1):1931. doi:
    Inserts
    Phytochrome B (PHYB Mustard Weed)
    Phytochrome interacting factor 3 (PIF3 Mustard Weed)
    Use
    Cre/Lox and Synthetic Biology
    Tags
    CreC, CreN, and SV40 NLS
    Expression
    Yeast
    Mutation
    N-terminal version of PhyB (AA 1-621)/ Mutation o…
    Promoter
    FBA1 and TDH3
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100537-plasmid-map-sequence-id-194456

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  12. pCFJ2307 - [1-2] ENTR - Fluor - ce-tagRFP-T(1 intron, syntrons(3), NLS(2), no_atg, no_stop)

    Plasmid
    #159856
    Purpose
    Three-fragment Gateway compatible flourophore for expression in C. elegans
    Depositor
    Christian Froekjaer-Jensen
    Publication
    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:
    Insert
    [1-2] ENTR - Fluor - ce-tagRFP-T(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
    Expression
    Worm
    Mutation
    Not applicable
    Available since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    159856-plasmid-map-sequence-id-318019

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  13. MSP2283

    Plasmid
    #70702
    Purpose
    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70702-plasmid-map-sequence-id-127769

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  15. pCR1056

    Plasmid
    #71824
    Purpose
    Express Streptococcus pyogenes dCas9 (D10A, H840A) carrying two C-terminal SV40 NLS
    Depositor
    Jacob Corn
    Publication
    Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    T7pr_6xHis-MBP-TEV-SPydCas9 (D10A,H840A)-2xNLS
    Use
    CRISPR
    Available since
    Feb. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    71824-plasmid-map-sequence-id-131017

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  16. pLenti-D156R LbCas12a-8xNLS

    Plasmid
    #248896
    Purpose
    Lentiviral vector expressing human codon-optimized D156R LbCas12a with 8xNLS
    Depositor
    Michael Aregger
    Insert
    D156R-LbCas12a-8xNLS-Myc-NeoR
    Use
    CRISPR and Lentiviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    D156R
    Promoter
    eIFa
    Available since
    Oct. 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    248896-plasmid-map-sequence-id-498938

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  17. pLenti-hyper LbCas12a-8xNLS

    Plasmid
    #248897
    Purpose
    Lentiviral vector expressing human codon-optimized hyper LbCas12a with 8xNLS
    Depositor
    Michael Aregger
    Insert
    hyper-LbCas12a-8xNLS-Myc-NeoR
    Use
    CRISPR and Lentiviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    D156R/D235R/E292R/D350R
    Promoter
    eIFa
    Available since
    Oct. 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    248897-plasmid-map-sequence-id-498940

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  18. plenti-ALFA-SpdCas9-2xNLS

    Plasmid
    #248898
    Purpose
    Lentiviral vector expressing human codon-optimized, ALFA-tagged SpdCas9 (deactivated DNAse domain)
    Depositor
    Michael Aregger
    Insert
    5xALFA-FLAG-SpdCas9-2xNLS-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D10A/H840A
    Promoter
    eIFa
    Available since
    Oct. 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    248898-plasmid-map-sequence-id-498941

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  19. plenti-ZIM3-SpdCas9-2xNLS

    Plasmid
    #248901
    Purpose
    Lentiviral vector expressing human codon-optimized SpdCas9 (deactivated DNAse domain) fused to ZIM3 KRAB domain
    Depositor
    Michael Aregger
    Insert
    ZIM3-FLAG-SpdCas9-2xNLS-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D10A/H840A
    Promoter
    eIFa
    Available since
    Oct. 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    248901-plasmid-map-sequence-id-498950

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Showing: 4961 - 4980 of 21078 results