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We narrowed to 6,359 results for: nls

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Showing: 4961 - 4980 of 6359 results

  2. pAAV_LP1B_St1Cas9_LMD-9_SpA_U6_sgRNA

    Plasmid
    #110624
    Purpose
    A single vector AAV-Cas9 system containing a liver-specific promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNA
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD-9
    sgRNA for St1Cas9
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    LP1B and hU6
    Available Since
    May 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    110624-plasmid-map-sequence-id-211314

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  3. pCAT105

    Plasmid
    #180510
    Purpose
    Plasmid expressing mammalian codon optimized engineered DpbCasX-R3, sgRNAv2 scaffold, and restriction sites to clone in new spacers
    Depositor
    Jennifer Doudna
    Article
    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6
    Inserts
    CasX sgRNAv2
    DpbCasX with R3 loop
    Use
    CRISPR
    Tags
    2x FLAG and SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and U6
    Available Since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180510-plasmid-map-sequence-id-359734

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  4. pYPQ266E

    Plasmid
    #161521
    Purpose
    Gateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 PmCDA1 mediated C-T base editing
    Depositor
    Yiping Qi
    Article
    Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41
    Insert
    zSpRYCas9(D10A)-PmCDA1-UGI
    Use
    CRISPR; Gateway compatible zsprycas9(d10a)-pmcda1…
    Tags
    3X FLAG, NLS
    Expression
    Plant
    Available Since
    Feb. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    161521-plasmid-map-sequence-id-325638

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  7. pCRI003-pGEM-LS-Bar-PgpdA-dSpCas9-VPR-TtrpC-LS

    Plasmid
    #140196
    Purpose
    Chromosomal integration of PgpdA-dSpCas9-VPR-TtrpC. Contains 1kb homology arms for Aspergillus nidulans and the bar gene for glufosinate selection. Filamentous fungi vector.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Inserts
    dSpCas9-VPR
    Bar
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
    Use
    CRISPR and Synthetic Biology; Fungal genome integ…
    Tags
    VPR (VP64-p65-Rta) , NLS
    Promoter
    PgpdA and PtrpC
    Available Since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    140196-plasmid-map-sequence-id-309706

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  10. pPB-VPR-dSpCas9[StaPL(TI)]

    Plasmid
    #111508
    Purpose
    Expresses a VPR transcriptional activator fused to the nuclease-deactivated S. pyogenes Cas9, which is governed by an inserted StaPL(TI) module, in mammalian cells. [TI = telaprevir inhibited].
    Depositor
    Michael Lin
    Article
    Jacobs et al Nat Methods. 2018 Jul;15(7):523-526. doi
    Insert
    VPR-dSpCas9[StaPL(TI)]
    Expression
    Mammalian
    Mutation
    The HCV NS3 protease carries F43L, Q80K, and S122…
    Promoter
    PGK
    Available Since
    July 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    111508-plasmid-map-sequence-id-214274

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  12. pYPQ230-RVR

    Plasmid
    #108861
    Purpose
    LbCpf1 Gateway entry plasmid with G548R, K554V and Y558R mutations
    Depositor
    Yiping Qi
    Article
    Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do
    Insert
    LbCpf1-RVR (LbCpf1 with G548R, K554V and Y558R mutations)
    Use
    CRISPR; Gateway compatible cpf1 entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    G548R, K554V and Y558R, Cpf1 is rice codon optimi…
    Available Since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    108861-plasmid-map-sequence-id-209929

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  13. PB-PE xCas9

    Plasmid
    #172652
    Purpose
    All-in-one prime editor piggyBac transposon (xCas9)
    Depositor
    Tobias Cantz
    Article
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Insert
    xCas9_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    A262T, R324L, S409I, E480K, E543D, M694I, E1219V
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    172652-plasmid-map-sequence-id-346754

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  16. pJEP315-pAAV-U6SaCas9gRNA(CREB)-CMV-SaCas9-DIO-pA

    Plasmid
    #113692
    Purpose
    U6 SaCas9 gRNA expression cassette containing a gRNA targeting the CREB gene, followed by a CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Inserts
    SaCas9 (NEWENTRY )
    SaCas9 gRNA Cassette (NEWENTRY )
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    NLS
    Promoter
    Cytomegalo Virus(CMV)
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113692-plasmid-map-sequence-id-229557

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  18. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)

    Plasmid
    #129028
    Purpose
    inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-mCherry and cloning backbone for sgRNA
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-hudTET1CD, SgRNA cloning site
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129028-plasmid-map-sequence-id-256131

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  19. pEMS1062

    Plasmid
    #29011
    Purpose
    The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.
    Depositor
    Elizabeth Simpson
    Article
    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414
    Insert
    Ple155-Cre/EGFP-NLS
    Use
    High copy number homologous recombination plasmid…
    Expression
    Mammalian
    Promoter
    MiniPromoter Ple155 from the human PCP2 gene
    Available Since
    Feb. 22, 2012
    Availability
    Academic Institutions and Nonprofits only
    29011-plasmid-map-sequence-id-13417

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Showing: 4961 - 4980 of 6359 results