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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,052 results for: nls

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Showing: 4961 - 4980 of 11052 results

  2. pCAG-hdAsCas12a(D908A)-NLS(nucleoplasmin)-gs-3xHA-gs-VPR (RTW876)

    Plasmid
    #114078
    Purpose
    CAG promoter expression plasmid for human codon optimized AsCas12a-VPR(1.1)
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized DNase-inactive (D908A) enAsCas12a fused to VPR activation domain
    Tags
    SV40 NLS
    Expression
    Mammalian
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    114078-plasmid-map-sequence-id-220564

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  3. JG1202: CAG-human dLbCpf1(D832A)-NLS-3xHA-P65

    Plasmid
    #104566
    Purpose
    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to p65 activation domain
    Depositor
    Keith Joung
    Publication
    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLbCpf1(D832A)-p65
    Tags
    3x HA and NLS
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG
    Available since
    Jan. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    104566-plasmid-map-sequence-id-260087

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  9. pET28a(+)v2_TwinStrep-SUMO-φC31(PhiC31)-8xHis

    Plasmid
    #246982
    Purpose
    Expresses E. coli codon-optimized TwinStrep-SUMO-tagged φC31(PhiC31) integrase with N- and C-terminal NLS, using an improved pET design from Shilling et al. 2020.
    Depositor
    Amro Hamdoun
    Insert
    φC31(PhiC31)
    Use
    Synthetic Biology
    Tags
    8xHis, Nucleoplasmin, SUMO, SV40, and TwinStrep
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    246982-plasmid-map-sequence-id-489535

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  10. PA-mCherry1-PCNA-19-SV40NLS-4

    Plasmid
    #57192
    Purpose
    Localization: Nucleus/Replication Origins, Excitation: 400 / 504, Emission: 515 / 517
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PCNA-19 SV40NLS (PCNA Human)
    Tags
    3x NLS and PA-mCherry1
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    57192-plasmid-map-sequence-id-228927

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  14. XLone-Puro beta-catenin P2A eGFP-NLS

    Plasmid
    #155189
    Purpose
    PiggybacTransposon-based tunable and temporal expression control of mutated beta-catenin and nuclear eGFP
    Depositor
    Xiaoping Bao
    Publication
    Chang et al Biomaterials. 2022 May 6;285:121569. doi
    Insert
    beta-catenin (Ctnnb1 Mouse)
    Use
    Unspecified; Piggybac transposon
    Tags
    P2A eGFP NLS
    Expression
    Mammalian
    Mutation
    Sites phosphorylated by GSK-3beta have been mutat…
    Promoter
    TRE3G
    Available since
    June 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    155189-plasmid-map-sequence-id-307963

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  17. pTES::NLS-GUS

    Plasmid
    #243808
    Purpose
    A reporter plasmid for transient expression in plant cells
    Depositor
    Hiroharu Banno
    Insert
    GUS
    Tags
    NLS
    Expression
    Plant
    Promoter
    CaMV 35S
    Available since
    Nov. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    243808-plasmid-map-sequence-id-488848

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Showing: 4961 - 4980 of 11052 results