We narrowed to 7,548 results for: ski

1. pET21a-C2m2-SNAP

   Plasmid

   #208773

   Purpose

   To express C2m2-SNAP in bacteria

   Depositor

   Urte Neniskyte

   Article

   Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

   Insert

   C2m2-SNAP (Mfge8 Mouse)
   

   Use

   Tags

   SNAP-tag

   Expression

   Bacterial

   Mutation

   Mutated 24 and 45 lysine to asparagine in C2 doma…

   Promoter

   T7 promoter

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-iCre-2A-cherry

   Plasmid

   #182246

   Purpose

   Expresses iCre linked to mCherry. The promoter is a fragment of the hdc gene promoter which drives pan neuronal expression.

   Depositor

   William Wisden

   Article

   Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.

   Insert

   pan neuronal gene promoter (hdc) fragment driving iCre recombinase and 2A linked to mCherry
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   fragment of histidine decarboxylase gene promoter…

   Available since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET30a-His6-ABP-LOXCAT

   Plasmid

   #139971

   Purpose

   Fusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2. Contains a albumin binding peptide (ABP).

   Depositor

   Vamsi Mootha

   Article

   Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

   Insert

   A fusion of lactate oxidase from A. viridans and catalase from E. coli
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWPI-LUC7L2-3xFLAG

   Plasmid

   #201641

   Purpose

   Over-expression of 3xFLAG-tagged human LUC7L2

   Depositor

   Alexis Jourdain, Vamsi Mootha

   Article

   Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

   Insert

   LUC7L2 (LUC7L2 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCC_09 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9-NLS-2A-Puro-WPRE

   Plasmid

   #139094

   Purpose

   Expresses human codon-optimized inactive SpCas9 fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   KRAB-dSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV.CAG.SF-iGluSnFR.A184V

   Plasmid

   #106199

   Purpose

   Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Insert

   SF-iGluSnFR.A184V
   

   Use

   AAV

   Tags

   Expression

   Mutation

   GltI: A184V

   Promoter

   CAG

   Available since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSH-EF1a-OsTIR1.F74A-mCherry-IRES-puro

   Plasmid

   #216247

   Purpose

   Express OsTIR1.F74A mutant with mCherry tag for AID2

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   OsTIR1.F74A
   

   Use

   CRISPR and TALEN; Human safe harbor locus (a avs1…

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pC129: pAAV.CMV-CasRx mCh

   Plasmid

   #203442

   Purpose

   Plasmid expressing active RfxCas13d with mCherry reporter for investigating CasRx activity

   Depositor

   Guei-Sheung Liu

   Article

   Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

   Insert

   RfxCas13d-T2A-mCherry
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. CRY-Gal∆DD (B1013)

   Plasmid

   #92035

   Purpose

   Expresses CRY2 (full-length, with endogenous NLS) fused to Gal4 (aa1-65 of Gal4BD), downstream of mCherry-IRES

   Depositor

   Chandra Tucker

   Article

   Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

   Insert

   CRY2 (CRY2 Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Fusion of plant CRY2 to Gal4 binding domain (AA1-…

   Promoter

   Available since

   July 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. MCP-NMS

    Plasmid

    #210705

    Purpose

    This plasmid express EF1a promoter driven MCP-NMS transactivation module

    Depositor

    Isaac Hilton

    Article

    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

    Insert

    MCP fused to transactivation domain from Human transcription factor NRF2, MRTF-A and STAT1
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N55K in MCP

    Promoter

    EF1a

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-flex-GFP-shRNA-GluN1

    Plasmid

    #182502

    Purpose

    Expresses shRNA against mouse NMDA receptor NR1 subunit. Flexed cassette driven by hdc (pan neuronal) gene promoter

    Depositor

    William Wisden

    Article

    Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.

    Insert

    pan neuronal promoter (hdc basal promoter) driving flexed-GFP-mir30-shRNA NR1 cassette
    

    Use

    AAV, Cre/Lox, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    fragment of histidine decarboxylase gene promoter…

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPN089

    Plasmid

    #91614

    Purpose

    Express sgRNA targeting human FURIN

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    FURIN (FURIN Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pAAV.CAG.SF-iGluSnFR.S72A

    Plasmid

    #106200

    Purpose

    Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-iGluSnFR.S72A
    

    Use

    AAV

    Tags

    Expression

    Mutation

    GltI: S72A

    Promoter

    CAG

    Available since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX Src-SH2

    Plasmid

    #46510

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Src (SRC Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 142-252

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. Basigin-isoform2-CD4d3+4-bio

    Plasmid

    #73129

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding Basigin-isoform2 with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    Basigin isoform 2 (BSG Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-hSyn-DIO-mCReP

    Plasmid

    #167503

    Purpose

    Conditional expression of the eIF2alpha phophatase, CReP (Ppp1r15b)

    Depositor

    Nicole Calakos

    Article

    Helseth et al Science. 2021 Apr 23;372(6540). pii: 372

    Insert

    Ppp1r15b (Ppp1r15b Mouse)
    

    Use

    AAV and Cre/Lox

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. CIB-VP64 (B1016)

    Plasmid

    #92037

    Purpose

    Expresses CIB1 (Full-length, with endogenous NLS) fused to VP64 activation domain (4xVP16 inserts)

    Depositor

    Chandra Tucker

    Article

    Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

    Insert

    CIB1-VP64 (CIB1 Mustard Weed)
    

    Use

    Tags

    Fusion of plant CIB1 with VP64 activation domain.…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-CAG-F14F15S-Switch

    Plasmid

    #99733

    Purpose

    Flp-based FLEX reporter (from RFP to GFP)

    Depositor

    Ian Wickersham

    Article

    Chatterjee et al Nat Neurosci. 2018 Apr;21(4):638-646. do

    Insert

    TagRFP and EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1/NDUFS2/myc-His

    Plasmid

    #127486

    Purpose

    Expresses myc-tagged NDUFS2 in mammalian cells

    Depositor

    Shashi Jain, Dieter Wolf

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS2 (NDUFS2 Human)
    

    Use

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-TRE-DIO-Casp3-TEV

    Plasmid

    #183766

    Purpose

    This plasmid is for use with neuronal cell-type selective activity tagging. The Caspase3 expression requires both neural activity and Cre recombinase.

    Depositor

    William Wisden

    Article

    Yu et al Science. 2022 Jul;377(6601):63-72. doi:

    Insert

    AAV transgene - tetO promoter-flex/DIO-Caspase3-TEV
    

    Use

    AAV, Cre/Lox, and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO promoter

    Available since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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