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Showing: 961 - 1000 of 6828 results

  1. mCerulean3-BclXL-Cb5-pEGFP-C1

    Plasmid
    #177414
    Purpose
    To express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-XL-Cb5: Bcl-XL with its membrane binding region swapped for Cb5 (ER targeting sequence).
    Depositor
    David Andrews
    Insert
    Bcl-XL-Cb5, BclX-cb5
    Use
    Tags
    mCerulean3
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177414-plasmid-map-sequence-id-348199

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  2. Venus-Bcl2-ActA-pEGFP-C1

    Plasmid
    #177419
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-ActA: Bcl-2 with its membrane binding region swapped for ActA (mitochondrial targeting sequence).
    Depositor
    David Andrews
    Insert
    Bcl-2-ActA, Bcl2-acta, Bcl-acta
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177419-plasmid-map-sequence-id-348305

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  3. Venus-Bcl2-cb5-pEGFP-C1

    Plasmid
    #177422
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-Cb5: Bcl-2 with its membrane binding region swapped for Cb5 (ER targeting sequence).
    Depositor
    David Andrews
    Insert
    Bcl-2-Cb5, Bcl2-cb5, Bcl-cb5
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177422-plasmid-map-sequence-id-353685

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  5. pRRlsinPGK_CREGFP_WPRE

    Plasmid
    #91789
    Purpose
    Constitutive expression of creGFP fusion using PGK promoter in a lentiviral backbone.
    Depositor
    Beverly Torok-Storb
    Insert
    Cre-GFP fusion protein
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    PGK
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    91789-plasmid-map-sequence-id-180615

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  6. pLenti-CMV-GFP-miRE_shSCR-Puro

    Plasmid
    #135689
    Purpose
    Constitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded scrambled control shRNA, Puromycin selection
    Depositor
    Florian Karreth
    Insert
    shScrambled
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135689-plasmid-map-sequence-id-327714

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  7. pLenti-EF1a-GFP-miRE_shRen-Puro

    Plasmid
    #135683
    Purpose
    Constitutive expression (EF1a promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Puromycin selection
    Depositor
    Florian Karreth
    Insert
    shRenilla
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135683-plasmid-map-sequence-id-327605

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  8. pLenti-EF1a-GFP-miRE_shRen-Blast

    Plasmid
    #135682
    Purpose
    Constitutive expression (EF1a promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Blasticidin selection
    Depositor
    Florian Karreth
    Insert
    shRenilla
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    135682-plasmid-map-sequence-id-327602

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  9. pLenti-CMV-GFP-miRE_shRen-Blast

    Plasmid
    #135684
    Purpose
    Constitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Blasticidin selection
    Depositor
    Florian Karreth
    Insert
    shRenilla
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    135684-plasmid-map-sequence-id-327610

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  10. pLenti-CMV-GFP-miRE_shTHUMPD3-Blast

    Plasmid
    #135690
    Purpose
    Constitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded shROSA26 (control shRNA), Blasticidin selection
    Depositor
    Florian Karreth
    Insert
    shRosa26
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135690-plasmid-map-sequence-id-327708

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  11. pLenti-CMV-GFP-miRE_shTHUMPD3-Puro

    Plasmid
    #135691
    Purpose
    Constitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded shROSA26 (control shRNA), Puromycin selection
    Depositor
    Florian Karreth
    Insert
    shRosa26
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135691-plasmid-map-sequence-id-327716

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  12. pLenti-CMV-GFP-miRE_shSCR-Blast

    Plasmid
    #135688
    Purpose
    Constitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded scrambled control shRNA, Blasticidin selection
    Depositor
    Florian Karreth
    Insert
    shScrambled
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135688-plasmid-map-sequence-id-327712

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  13. pLenti-CMV-GFP-miRE_shRen-Puro

    Plasmid
    #135685
    Purpose
    Constitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Puromycin selection
    Depositor
    Florian Karreth
    Insert
    shRenilla
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    135685-plasmid-map-sequence-id-327612

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  14. CAG-GFP/cre

    Plasmid
    #49054
    Purpose
    Retroviral vector contains CAG promoter , CMV enhancer and a large synthetic intron that used to allow the ubiquitous expression of GFP-fused Cre recombinase
    Depositor
    Fred Gage
    Article
    Tashiro et al Nat Protoc. 2006;1(6):3049-55.
    Insert
    GFP/Cre
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Nov. 4, 2013
    Availability
    Academic Institutions and Nonprofits only
    49054-plasmid-map-sequence-id-72065

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  15. Venus-Bik-DD-pEGFP-C1

    Plasmid
    #177429
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, phosphomimetic mutant. T33D and S35D mutations made to Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Threonine 33 and Serine 35 replaced with aspartic…
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177429-plasmid-map-sequence-id-348456

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  17. Venus-Bik-AA-pEGFP-C1

    Plasmid
    #177430
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with 2 sites of phosphorylation mutated to alanine. T33A and S35A mutations made to Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Threonine 33 and Serine 35 replaced with alanine
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177430-plasmid-map-sequence-id-348458

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  18. pAAV hSyn GFP-Cre

    Plasmid
    #175381
    Purpose
    expresses GFP-Cre under the control of a human synapsin promoter
    Depositor
    Jason Aoto
    Insert
    GFP-Cre recombinase
    Use
    AAV
    Tags
    Cre is fused to GFP. GFP is fused to additional n…
    Expression
    Mutation
    Promoter
    human synapsin
    Available since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    175381-plasmid-map-sequence-id-348392

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  19. pRK5_msfGFP-HMGB1-Mut-11G3F3G

    Plasmid
    #237626
    Purpose
    For overexpression of msfGFP-HMGB1-Mut-11G3F3G
    Depositor
    Denes Hnisz
    Insert
    HMGB1-Mut-11G3F3G (HMGB1 Human)
    Use
    Tags
    msfGFP
    Expression
    Mammalian
    Mutation
    11G3F3G
    Promoter
    Available since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    237626-plasmid-map-sequence-id-471804

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  20. pRK5_msfGFP-HMGB1-Mut-FtoA

    Plasmid
    #237620
    Purpose
    For overexpression of msfGFP-HMGB1-Mut-FtoA
    Depositor
    Denes Hnisz
    Insert
    HMGB1-Mut-FtoA (HMGB1 Human)
    Use
    Tags
    msfGFP
    Expression
    Mammalian
    Mutation
    F to A
    Promoter
    Available since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    237620-plasmid-map-sequence-id-471763

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  21. pRK5_msfGFP-HMGB1-Mut-F12A

    Plasmid
    #237623
    Purpose
    For overexpression of msfGFP-HMGB1-Mut-F12A
    Depositor
    Denes Hnisz
    Insert
    HMGB1-Mut-F12A (HMGB1 Human)
    Use
    Tags
    msfGFP
    Expression
    Mammalian
    Mutation
    F12A
    Promoter
    Available since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    237623-plasmid-map-sequence-id-471746

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  22. pRK5_msfGFP-HMGB1-Mut-C16toA

    Plasmid
    #237627
    Purpose
    For overexpression of msfGFP-HMGB1-Mut-C16toA
    Depositor
    Denes Hnisz
    Insert
    HMGB1-Mut-C16toA (HMGB1 Human)
    Use
    Tags
    msfGFP
    Expression
    Mammalian
    Mutation
    C16A
    Promoter
    Available since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    237627-plasmid-map-sequence-id-471723

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  23. pRK5_msfGFP-HMGB1-Mut-F13A

    Plasmid
    #237624
    Purpose
    For overexpression of msfGFP-HMGB1-Mut-F13A
    Depositor
    Denes Hnisz
    Insert
    HMGB1-Mut-F13A (HMGB1 Human)
    Use
    Tags
    msfGFP
    Expression
    Mammalian
    Mutation
    F13A
    Promoter
    Available since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    237624-plasmid-map-sequence-id-471716

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  25. pAc5.1B-EGFP-DmSmaug_584-999-V5His6_C

    Plasmid
    #146003
    Purpose
    Insect Expression of DmSmaug_584-999
    Depositor
    Elisa Izaurralde
    Insert
    DmSmaug_584-999 (smg Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    5 silent mutations and a deletion of the AA 961-9…
    Promoter
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    146003-plasmid-map-sequence-id-288448

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  26. pAc5.1B-EGFP-DmSmaug_763-999-V5His6_C

    Plasmid
    #146004
    Purpose
    Insect Expression of DmSmaug_763-999
    Depositor
    Elisa Izaurralde
    Insert
    DmSmaug_763-999 (smg Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    2 silent mutations and a deletion of the AA 961-9…
    Promoter
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    146004-plasmid-map-sequence-id-288449

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  27. pAc5.1B-EGFP-DmSmaug-A642H-V5His6_C

    Plasmid
    #146005
    Purpose
    Insect Expression of DmSmaug-A642H
    Depositor
    Elisa Izaurralde
    Insert
    DmSmaug-A642H (smg Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    10 silent mutations and a deletion of the AA 961-…
    Promoter
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    146005-plasmid-map-sequence-id-288450

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  28. pAc5.1B-EGFP-DmSmaug-K606AR609A-V5His6_C

    Plasmid
    #146007
    Purpose
    Insect Expression of DmSmaug-K606AR609A
    Depositor
    Elisa Izaurralde
    Insert
    DmSmaug-K606AR609A (smg Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    10 silent mutations and a deletion of the AA 961-…
    Promoter
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    146007-plasmid-map-sequence-id-288452

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  29. pAc5.1B-EGFP-DmUPF2-siRNAres-V5His6_E

    Plasmid
    #146166
    Purpose
    Insect Expression of DmUPF2-siRNAres
    Depositor
    Elisa Izaurralde
    Insert
    DmUPF2-siRNAres (Upf2 Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    3 non silent mutations: L1022P, V1097L and N1213S…
    Promoter
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    146166-plasmid-map-sequence-id-336056

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  30. pAc5.1B-EGFP-DmSmaug_1-762-V5His6_C

    Plasmid
    #146001
    Purpose
    Insect Expression of DmSmaug_1-762
    Depositor
    Elisa Izaurralde
    Insert
    DmSmaug_1-762 (smg Fly)
    Use
    Tags
    V5-6xHis
    Expression
    Insect
    Mutation
    NP_523987.1, 7 silent mutations compared to the s…
    Promoter
    Available since
    May 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    146001-plasmid-map-sequence-id-333046

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  31. pSAH52 - [DDp | gfp | tbb-2 UTR]

    Plasmid
    #200328
    Purpose
    gfp BioPart for DD neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.
    Depositor
    Christian Froekjaer-Jensen
    Insert
    [DDp | gfp | tbb-2 UTR]
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    WBGene00007304, ttr-39
    Available since
    June 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    200328-plasmid-map-sequence-id-396872

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  32. pSAH48 - [ADLp | gfp | tbb-2 UTR]

    Plasmid
    #200324
    Purpose
    gfp BioPart for ADL neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.
    Depositor
    Christian Froekjaer-Jensen
    Insert
    [ADLp | gfp | tbb-2 UTR]
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    WBGene00011644, T09B9.3
    Available since
    June 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    200324-plasmid-map-sequence-id-396867

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  33. p006-GFP-Logic-AND

    Plasmid
    #112237
    Purpose
    GFP from jellyfish Aquorea sp. under D61 AND gate-like ara+lac promoter with AraC repressor; lacI repressor; kanamycin resistant.
    Depositor
    Jaroslaw Bryk
    Article
    Siddall et al bioRxiv 2021.06.20.449138
    Inserts
    GFP under control of doubly-repressible promoter
    GFP
    araC
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    AND-gate and pLacI
    Available since
    Aug. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    112237-plasmid-map-sequence-id-218819

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Showing: 961 - 1000 of 6828 results