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POSV801-SP44-SnaABCDEinOT1T2

Plasmid

#225057

Purpose

Heterologous expression in Streptomyces with inactive SnaE

Depositor

Wilfred van der Donk

Article

Yu et al ACS Cent Sci. 2024 Jun 3;10(6):1242-1250

Insert

inactivated SnaE

Expression

Bacterial

Mutation

deltaA46-V801

Promoter

SP44

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAM248

Plasmid

#226892

Purpose

6xHis-PSP-GS-PANN(75-150)-(5aaGS)-Msp1(36-362)_W166A/Y167A

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

msp1 (MSP1 Budding Yeast)

Tags

His-Precission-PANN

Expression

Bacterial

Mutation

W166A/Y167A

Promoter

T7

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAM257

Plasmid

#226901

Purpose

6xHis-PSP-MBP-Pex3(40-441)

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

pex3 (PEX3 Budding Yeast)

Tags

6xHis-PSP-MBP

Expression

Bacterial

Mutation

amino acids 40-441

Promoter

T7

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM256

Plasmid

#226900

Purpose

mEOS3.2-GS-SUMO-1(3-103)-Pex15(1-309)-PSP-GSS-FLAG-6xHis

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

mEOS3.2

Tags

GS-SUMO-1(3-103)-Pex15(1-309)-PSP-GSS-FLAG-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM255

Plasmid

#226899

Purpose

mEOS3.2-GS-MBP-10xN-Pex15(1-309)-PSP-GSS-FLAG-6xHis

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

mEOS3.2

Tags

mEOS3.2-GS-MBP-10xN-Pex15(1-309)-Prescission-GSS-…

Expression

Bacterial

Promoter

T7

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM249

Plasmid

#226893

Purpose

Pex15(43-309)-PSP-GSS-FLAG-6xHis

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

pex15 (PEX15 Budding Yeast)

Tags

prescission -GSS-FLAG-6xHis

Expression

Bacterial

Mutation

Pex15(aa 43-309)

Promoter

T7

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM262

Plasmid

#226906

Purpose

20aa^Pex15(1-253)-PSP-6xHis-FLAG

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

pex15 (PEX15 Budding Yeast)

Tags

-PSP-6xHis-FLAG

Expression

Bacterial

Mutation

1-253

Promoter

T7

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM252

Plasmid

#226896

Purpose

MBP-Pex15(1-327)-PSP-GSS-FLAG-6xHis

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

pex15 (PEX15 Budding Yeast)

Tags

MBP and prescission -GSS-FLAG-6xHis

Expression

Bacterial

Mutation

Pex15(aa 1-327))

Promoter

T7

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM245

Plasmid

#226889

Purpose

6xHis-PSP-GS-PANN(75-150)-(5aaGS)-Msp1(36-362)

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

msp1 (MSP1 Budding Yeast)

Tags

His-Precission-PANN

Expression

Bacterial

Promoter

T7

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM250

Plasmid

#226894

Purpose

Pex15(1-253)-PSP-6xHis-FLAG

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

pex15 (PEX15 Budding Yeast)

Tags

prescission -GSS-FLAG-6xHis

Expression

Bacterial

Mutation

Pex15(aa 1-253)

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM251

Plasmid

#226895

Purpose

MBP-Pex15(1-309)-PSP-GSS-FLAG-6xHis

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

pex15 (PEX15 Budding Yeast)

Tags

MBP and prescission -GSS-FLAG-6xHis

Expression

Bacterial

Mutation

Pex15(aa 1-309)

Promoter

T7

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM247

Plasmid

#226891

Purpose

6xHis-PSP-GS-Hcp1(1-162)-(6aaSG)-Msp1(36-362)

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

msp1 (MSP1 Budding Yeast)

Tags

His-Precission-Hcp

Expression

Bacterial

Mutation

aa 36-362

Promoter

T7

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM246

Plasmid

#226890

Purpose

6xHis-PSP-GS-ccHex-(9aaSG)-Msp1(36-362)

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

msp1 (MSP1 Budding Yeast)

Tags

His-Precission-cchex

Expression

Bacterial

Mutation

aa 36-362

Promoter

T7

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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POSV801-SP44-SnaABCDEOinT1T2

Plasmid

#225058

Purpose

Heterologous expression in Streptomyces with inactive SnaO

Depositor

Wilfred van der Donk

Article

Yu et al ACS Cent Sci. 2024 Jun 3;10(6):1242-1250

Insert

inactivated SnaO

Expression

Bacterial

Mutation

deltaR51-V511

Promoter

SP44

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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POSV801-SP44-SnaABCDEOT1T2

Plasmid

#225055

Purpose

Heterologous expression in Streptomyces for threopeptin biosynthetic gene cluster

Depositor

Wilfred van der Donk

Article

Yu et al ACS Cent Sci. 2024 Jun 3;10(6):1242-1250

Insert

SnaA

Expression

Bacterial

Mutation

WT

Promoter

SP44

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF-coSnaA-TEV-His_His-TEV-SnaC

Plasmid

#225059

Purpose

recombinant expression of SnaA in E. coli

Depositor

Wilfred van der Donk

Article

Yu et al ACS Cent Sci. 2024 Jun 3;10(6):1242-1250

Insert

SnaA

Expression

Bacterial

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pGWT35S-TP-UnaG

Plasmid

#203484

Purpose

For particle bombardment. A plastid transit peptide fused UnaG under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

Depositor

Yutaka Kodama

Article

Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

Insert

Plastid transit peptide fused UnaG

Expression

Bacterial and Plant

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NcQDE2_506-938-D603K_M

Plasmid

#146962

Purpose

Bacterial Expression of NcQde-2_506-938-D603K

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

NcQde-2_506-938-D603K

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NcQDE2_506-938-K638E_M

Plasmid

#146963

Purpose

Bacterial Expression of NcQde-2_506-938-K638E

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

NcQde-2_506-938-K638E

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NcQDE2 _506-938-A602E_M

Plasmid

#146964

Purpose

Bacterial Expression of NcQde-2_506-938-A602E

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

NcQde-2_506-938-A602E

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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