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pAAV-G12-AkaLuc-P2A-Proinsulin

Plasmid

#241776

Purpose

Tested on HeLa for bioluminescence, and used for production of AAV G12-Akaluc-P2A-Proinsulin for mice.

Depositor

Vladislav Verkhusha

Article

Kasatkina et al Nat Commun. 2025 Jul 14;16(1):6469. doi:

Insert

AkaLuc-P2A-Proinsulin

Use

AAV

Promoter

12xUAS with TATA-box minimal promoter

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDisplay-CMV-GRAB_LoX3-mut

Plasmid

#234651

Purpose

This plasmid encodes a mutated version of the genetically encoded chemokine biosensor LoX3-1.0, which is designed to be non-responsive to chemokine binding.

Depositor

Miao Jing

Article

Xi et al Immunity. 2025 Sep 9;58(9):2320-2335.e9.

Insert

GPCR activation-based mutant chemokine CXCL9-11 sensor GRAB_LoX3-mut

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGIG-Sema7A-ΔSEMA

Plasmid

#190647

Purpose

Expresses Mouse Sema7A with SEMA domain deleted

Depositor

Andrew Newman, Victor Tarabykin

Article

Bessa et al Nat Commun. 2024 Aug 16;15(1):7059. doi:

Insert

Sema7A (Sema7a Mouse)

Expression

Mammalian

Mutation

SEMA Domain Deleted

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGIG-Sema7A-ΔIG

Plasmid

#190648

Purpose

Expresses Mouse Sema7A with IG Domain Deleted

Depositor

Andrew Newman, Victor Tarabykin

Article

Bessa et al Nat Commun. 2024 Aug 16;15(1):7059. doi:

Insert

Sema7A (Sema7a Mouse)

Expression

Mammalian

Mutation

IG Domain Deleted

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGIG-Sema7A-ΔPSI

Plasmid

#190649

Purpose

Expresses Mouse Sema7A with PSI domain deleted

Depositor

Andrew Newman, Victor Tarabykin

Article

Bessa et al Nat Commun. 2024 Aug 16;15(1):7059. doi:

Insert

Sema7A (Sema7a Mouse)

Expression

Mammalian

Mutation

PSI Domain Deleted

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGIG-Sema7A-KCE

Plasmid

#190651

Purpose

Expresses Mouse Sema7A with RGD motif mutated to KCE

Depositor

Andrew Newman, Victor Tarabykin

Article

Bessa et al Nat Commun. 2024 Aug 16;15(1):7059. doi:

Insert

Sema7A (Sema7a Mouse)

Expression

Mammalian

Mutation

RGD mutated to KCE

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-sp-myc-hSema4D

Plasmid

#190654

Purpose

Expresses Human Sema4D with N terminal myc after signal peptide

Depositor

Andrew Newman, Victor Tarabykin

Article

Bessa et al Nat Commun. 2024 Aug 16;15(1):7059. doi:

Insert

Sema4D (SEMA4D Human)

Tags

myc

Expression

Mammalian

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR2b ITGB1-tail-mTurquoise2

Plasmid

#145142

Purpose

Shuttle vector with mTurquoise2-tagged ITGB1 cytoplasmic tail as an insert

Depositor

Johanna Ivaska

Article

Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

Insert

ITGB1-tail-mTurquoise2 (ITGB1 Human)

Use

Entry vector for gateway cloning

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR2b ITGA2-tail-YPet

Plasmid

#145143

Purpose

Shuttle vector with YPet-tagged ITGA2 wild-type cytoplasmic tail as an insert

Depositor

Johanna Ivaska

Article

Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

Insert

ITGA2-tail-YPet (ITGA2 Human)

Use

Entry vector for gateway cloning

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR2b ITGA2-tail-YPet (GFFKR/GAAKR)

Plasmid

#145144

Purpose

Shuttle vector with YPet-tagged ITGA2 cytoplasmic tail as an insert with a GFFKR/GAAKR mutation

Depositor

Johanna Ivaska

Article

Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

Insert

ITGA2-tail-YPet (GFFKR/GAAKR) (ITGA2 Human)

Use

Entry vector for gateway cloning

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgDHFR

Plasmid

#233898

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting DHFR

Depositor

Jason Cantor

Article

Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

Insert

sgRNA targeting DHFR (DHFR Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-CCR5

Plasmid

#222309

Purpose

Synchronize the trafficking of CCR5 from the ER.

Depositor

Franck Perez

Article

Boncompain et al Sci Adv. 2019 Oct 16;5(10):eaax0821. doi

Insert

Streptavidin-KDEL and CCR5 fused to SBP-EGFP (CCR5 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR223-TAF5-∆ex8

Plasmid

#209048

Purpose

Gateway-compatible entry vector, with insert of the TAF5 isoform lacking the alternative exon-8 (∆ex8)

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

TAF5 deltaexon-8

Use

Gateway entry vector

Expression

Mammalian

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-PGM2_sgRNA2

Plasmid

#201617

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

PGM2 (PGM2 Human)

Use

CRISPR and Lentiviral

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-PGM2_sgRNA1

Plasmid

#201616

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

PGM2 (PGM2 Human)

Use

CRISPR and Lentiviral

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-UCK2_sgRNA2

Plasmid

#201633

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

UCK2 (UCK2 Human)

Use

CRISPR and Lentiviral

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-RPE_sgRNA2

Plasmid

#201619

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

RPE (RPE Human)

Use

CRISPR and Lentiviral

Available Since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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B*46:01:01

Plasmid

#203250

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

B*46:01:01 (HLA-B Human)

Expression

Mammalian

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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B*53:01:01

Plasmid

#203249

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

B*53:01:01 (HLA-B Human)

Expression

Mammalian

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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B*08:01:01

Plasmid

#203251

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

B*08:01:01 (HLA-B Human)

Expression

Mammalian

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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