We narrowed to 14,090 results for: cas9

1. BCJJ180

   Plasmid

   #117516

   Purpose

   Cas9_4 Golden Gate Level 0

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BsaI:AATG:Cas9_4:GCTT:BsaI
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Plant

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pICSL90016

   Plasmid

   #117514

   Purpose

   Cas9_2 Golden Gate Level 0

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BsaI:AATG:Cas9_2:GCTT:BsaI
   

   Use

   CRISPR

   Tags

   NLS_SV40

   Expression

   Plant

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSCBE2

   Plasmid

   #133965

   Purpose

   Expresses BE2 CRISPR base editor and gRNA scaffold in Streptomyces

   Depositor

   Yuhui Sun

   Article

   Zhong et al bioRxiv 630137

   Insert

   BE2
   

   Use

   CRISPR and Synthetic Biology

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAH01SpR

   Plasmid

   #128159

   Purpose

   pCAH01 derivative (Ptet inducible promoter) with ahp (kanamycin resistance) CDS replaced with the aadA (streptomycin/spectinomycin resistance) CDS

   Depositor

   Calvin Henard

   Article

   Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   Ptet

   Available Since

   July 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. vHB179

   Plasmid

   #193458

   Purpose

   3xFLAG endogenous tagging of pandoravirus/mollivirus genes

   Depositor

   Chantal Abergel

   Article

   Bisio et al Nat Commun. 2023 Jan 26;14(1):428. doi:

   Insert

   3xFLAG
   

   Use

   Pandoravirus/mollivirus expression

   Promoter

   ---

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSABEn

   Plasmid

   #133969

   Purpose

   Expresses ABEn CRISPR base editor and gRNA scaffold in Streptomyces

   Depositor

   Yuhui Sun

   Article

   Zhong et al bioRxiv 630137

   Insert

   ABEn
   

   Use

   CRISPR and Synthetic Biology

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCF525-AcrIIA5_gb36_lenti

   Plasmid

   #138306

   Purpose

   Lenti expression of AcrIIA5

   Depositor

   Jennifer Doudna

   Article

   Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

   Insert

   AcrIIA5
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   March 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMLS236

   Plasmid

   #73684

   Purpose

   SapTrap 3-site Destination vector for internal GFP tagging with embedded Cbr-unc-119 selectable marker

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   GFP + Cbr-unc-119
   

   Use

   CRISPR and Cre/Lox

   Tags

   GFP

   Expression

   Worm

   Available Since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. U6>XX

   Plasmid

   #87542

   Purpose

   Component 1 of 2 for U6>sgRNA cassette OSO-PCR

   Depositor

   Lionel Christiaen

   Article

   Gandhi et al Dev Biol. 2017 May 1;425(1):8-20. doi: 1

   Type

   Empty backbone

   Available Since

   Jan. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. YN2_1_IL50_HOlocus

    Plasmid

    #194426

    Purpose

    Expresses Cas9 and sgRNA cassettes for targeting the HO locus in yeast Saccharomyces cerevisiae

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

    Insert

    Cas9
    

    Expression

    Yeast

    Mutation

    NA

    Available Since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. AP682-1

    Plasmid

    #70050

    Purpose

    Expresses TEV::eGFP::myc::3Xflag in bacteria

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

    Insert

    eGFP
    

    Tags

    TEV and myc::3Xflag

    Expression

    Bacterial

    Available Since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDD399

    Plasmid

    #91821

    Purpose

    AID CT-tag linker donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    Auxin-inducible degron (AID)
    

    Use

    CRISPR

    Expression

    Worm

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMLS235

    Plasmid

    #73683

    Purpose

    SapTrap 3-site Destination vector for C-terminal GFP tagging with embedded Cbr-unc-119 selectable marker

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    GFP + Cbr-unc-119
    

    Use

    CRISPR and Cre/Lox

    Expression

    Worm

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. lentiGuide-Puro.3xBsmBI

    Plasmid

    #196709

    Purpose

    Backbone for cloning guide libraries with untranscribed barcodes and a tracrRNA of choice

    Depositor

    Bernhard Schmierer

    Article

    Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMLS360

    Plasmid

    #73687

    Purpose

    Punc-47::2xNLS-FLP-D5 Expression vector

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    Punc-47::2xNLS-FLP-D5::let-858 3'-UTR
    

    Use

    Flp/frt

    Expression

    Worm

    Promoter

    Punc-47

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCfB8383

    Plasmid

    #126917

    Purpose

    Plasmid containing integration cassette at XI-3 integration site in Saccharomyces cerevisiae for overexpression of tHMG1

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    tHMG1
    

    Expression

    Yeast

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-T7-SpG-P2A-mScarlet (MNW006)

    Plasmid

    #174136

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized SpG with BPNLS-3xFLAG-P2A-mScarlet
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-mScarlet

    Expression

    Mammalian

    Mutation

    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

    Promoter

    CMV and T7

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDD375

    Plasmid

    #91825

    Purpose

    Codon-optimized mKate2 donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    mKate2-C1
    

    Use

    CRISPR

    Expression

    Worm

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. lentiCRISPRv2-7SK-Blast

    Plasmid

    #183620

    Purpose

    lentiCRISPRv2 derivative with modified 7SK promoter driving sgRNA and encoding Blasticidin resistance. Compatible with inserts designed for lentiCRISPRv2.

    Depositor

    Bill Sugden

    Article

    Hutcheson et al ACS Synth Biol. 2022 Jun 29. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    Modified 7SK

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMLS719

    Plasmid

    #188894

    Purpose

    miniMos Arm sgRNA expression vector

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

    Insert

    PU6(K09B11.12)::miniMos Arm sgRNA
    

    Expression

    Worm

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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