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pJUMP24_T24_pRham-ilvA-relE_RBS3

Plasmid

#201532

Purpose

Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

Depositor

Dan Park

Article

Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d

Insert

ilvA-relE_RBS3

Use

Synthetic Biology

Promoter

PrhaBAD (rhamnose)

Available Since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2572

Plasmid

#195683

Purpose

Expression/purification of 6xHis-TEV-MlaC(V171R)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(V171R)

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1223

Plasmid

#195688

Purpose

Expression/purification of 6xHis-TEV-MlaDdelta173-183

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaD

Expression

Bacterial

Mutation

6xHis-TEV-MlaDdelta173-183

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1909

Plasmid

#195690

Purpose

Expression/purification of 6xHis-TEV-MlaD(F118A)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaD

Expression

Bacterial

Mutation

6xHis-TEV-MlaD(F118A)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1910

Plasmid

#195691

Purpose

Expression/purification of 6xHis-TEV-MlaD(L123A)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaD

Expression

Bacterial

Mutation

6xHis-TEV-MlaD(L123A)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2555

Plasmid

#195673

Purpose

Expression/purification of 6xHis-TEV-MlaC(G170K)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(G170K)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2556

Plasmid

#195674

Purpose

Expression/purification of 6xHis-TEV-MlaC(I130D)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(I130D)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2559

Plasmid

#195675

Purpose

Expression/purification of 6xHis-TEV-MlaC(L76D)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(L76D)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2560

Plasmid

#195676

Purpose

Expression/purification of 6xHis-TEV-MlaC(N155A)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(N155A)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2561

Plasmid

#195677

Purpose

Expression/purification of 6xHis-TEV-MlaC(N179E)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(N179E)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2563

Plasmid

#195678

Purpose

Expression/purification of 6xHis-TEV-MlaC(R134E)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(R134E)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2564

Plasmid

#195679

Purpose

Expression/purification of 6xHis-TEV-MlaC(R147D)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(R147D)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2565

Plasmid

#195680

Purpose

Expression/purification of 6xHis-TEV-MlaC(R153A)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(R153A)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2566

Plasmid

#195681

Purpose

Expression/purification of 6xHis-TEV-MlaC(R186A)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(R186A)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2568

Plasmid

#195682

Purpose

Expression/purification of 6xHis-TEV-MlaC(T176R)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(T176R)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2573

Plasmid

#195684

Purpose

Expression/purification of 6xHis-TEV-MlaC(Y72A)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(Y72A)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2574

Plasmid

#195685

Purpose

Expression/purification of 6xHis-TEV-MlaC(Y82K)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(Y82K)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2576

Plasmid

#195686

Purpose

Expression/purification of 6xHis-TEV-MlaC(Y164R)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(Y164R)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1224

Plasmid

#195687

Purpose

Expression/purification of 6xHis-TEV-MlaDdelta153-183

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaD

Expression

Bacterial

Mutation

6xHis-TEV-MlaDdelta153-183

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2537

Plasmid

#195658

Purpose

Complementation of MlaC(R153A)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

MlaC(R153A)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge