Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 6,962 results for: pcDNA3.1+

Clear keywords
Showing: 5001 - 5020 of 6962 results

  5. pSFV_SRF-NLS-VP64 (3'UTR)_RLEloop

    Plasmid
    #240258
    Purpose
    Attenuated PROTEUS vector with SRF-VP64 transgene. Package VLVs with an envelope-expressing vector such as pCMV-VSV-G, or a transgene-responsive VSV-G expression vector.
    Depositor
    Gregory Neely
    Publication
    Cole et al Nat Commun. 2025 May 7;16(1):4250. doi:
    Insert
    SRF
    Tags
    NLS-VP64
    Expression
    Mammalian
    Mutation
    SRF[aa1-265]; VP64 = 4*VP16[aa437-447]
    Promoter
    SFV subgenomic promoter
    Available since
    Feb. 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    240258-plasmid-map-sequence-id-495356

    Enlarge

  7. pXeDNA3.1-LacZ

    Plasmid
    #242746
    Purpose
    pXeDNA3-LacZ TA-cloning vector for dual expression: CMV enhancer/promoter in mammalian cells and Xenopus β-globin 5'/3'UTRs in oocytes; BGHpA, AmpR, NeoR, lacZα for blue-white screening.
    Depositor
    Luis Yanez-Guerra
    Publication
    Guan et al MicroPubl Biol. 2025 Dec 1;2025:10.17912
    Type
    Empty backbone
    Use
    Xenopus oocyte expression
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    242746-plasmid-map-sequence-id-487866

    Enlarge

  8. TEV-HRV-3C Proteases-mCherry

    Plasmid
    #243795
    Purpose
    Encodes for mammalian expression of TEV and HRV-3C protease inputs. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.
    Depositor
    Cole DeForest
    Publication
    Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TEV and HRV-3C proteases
    Expression
    Mammalian
    Available since
    Oct. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    243795-plasmid-map-sequence-id-486454

    Enlarge

  9. TVMV-TEV-HRV-3C Proteases-mCherry

    Plasmid
    #243798
    Purpose
    Encodes for mammalian expression of TVMV, TEV and HRV-3C protease inputs. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.
    Depositor
    Cole DeForest
    Publication
    Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TVMV, TEV and HRV-3C proteases
    Expression
    Mammalian
    Available since
    Oct. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    243798-plasmid-map-sequence-id-486451

    Enlarge

  10. ACE2-HA-Linker-FLAG

    Plasmid
    #240602
    Purpose
    Encodes doubly tagged ACE2 protein. HA on the N-terminal added after the signal peptide (a.a 1-17) and FLAG tag at the end of the insert. A linker sequence was added after the HA tag.
    Depositor
    Zaid Abassi
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    FLAG tag and HA tag (after the signal peptide)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    240602-plasmid-map-sequence-id-482812

    Enlarge

  12. pcDNA-mito-GreenPy1Low

    Plasmid
    #239071
    Purpose
    Mammalian expression of mitochondria-targeted low affinity green fluorescent pyruvate biosensor GreenPy1Low
    Depositor
    Robert Campbell
    Publication
    Imai et al bioRxiv 2025.04.17.649293
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GreenPy1Low
    Expression
    Mammalian
    Available since
    June 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    239071-plasmid-map-sequence-id-476955

    Enlarge

  13. pcDNA-GreenPy1Low

    Plasmid
    #239061
    Purpose
    Mammalian expression of low affinity green fluorescent pyruvate biosensor GreenPy1Low
    Depositor
    Robert Campbell
    Publication
    Imai et al bioRxiv 2025.04.17.649293
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GreenPy1Low
    Expression
    Mammalian
    Available since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239061-plasmid-map-sequence-id-476566

    Enlarge

  14. pcDNA-ApplePy1Lowest

    Plasmid
    #239067
    Purpose
    Mammalian expression of lowest affinity red fluorescent pyruvate biosensor ApplePy1Lowest
    Depositor
    Robert Campbell
    Publication
    Imai et al bioRxiv 2025.04.17.649293
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ApplePy1Lowest
    Expression
    Mammalian
    Available since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239067-plasmid-map-sequence-id-476343

    Enlarge

  15. pStreptagII 3C SMO

    Plasmid
    #234995
    Purpose
    Expression of a Strep-tag II labeled on its N-terminus receptor, with the Smoothened (Smo) receptor
    Depositor
    Monica Gonzalez-Magaldi, Daniel Leahy
    Publication
    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SMO (SMO Human)
    Tags
    Streptag-II, HRV 3C site
    Expression
    Mammalian
    Available since
    April 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    234995-plasmid-map-sequence-id-468950

    Enlarge

  16. pHA TST 3C SMO EABR

    Plasmid
    #234989
    Purpose
    For production of Extracellular Vesicles (EVs), with the receptor Smoothened (Smo), Twin-Strep-tag (TST), and an HA tag at its N-terminus on their surface
    Depositor
    Monica Gonzalez-Magaldi, Daniel Leahy
    Publication
    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SMO-EABR (SMO Human)
    Tags
    HA, Twin-Strep-Tag, HRV 3C site
    Expression
    Mammalian
    Available since
    April 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    234989-plasmid-map-sequence-id-468945

    Enlarge

  18. pHA StreptagII 3C NIS EABR

    Plasmid
    #234988
    Purpose
    For production of Extracellular Vesicles (EVs), with a Strep-tag II labeled Sodium-iodide symporter (NIS), and an HA epitope at its N-terminus on their surface
    Depositor
    Monica Gonzalez-Magaldi, Daniel Leahy
    Publication
    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NIS-EABR (SLC5A5 Human)
    Tags
    HA, Streptag-II, HRV 3C site
    Expression
    Mammalian
    Available since
    March 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    234988-plasmid-map-sequence-id-466789

    Enlarge

  19. memGRKct-Nluc

    Plasmid
    #234753
    Purpose
    Mammalian expression of plasma membrane anchored (GAP43) GRKct-Nluc
    Depositor
    Nevin Lambert
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    memGRKct-Nluc
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234753-plasmid-map-sequence-id-466032

    Enlarge

Showing: 5001 - 5020 of 6962 results