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We narrowed to 6,024 results for: crispr cas9 expression plasmids

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Showing: 5061 - 5080 of 6024 results

  2. pCAG-EGxxFP

    Plasmid
    #50716
    Purpose
    5’ and 3’ EGFP fragments that shares 482bp were placed under ubiquitous CAG promoter. Used for validation of gRNA sequences by DSB mediated EGFP reconstitution.
    Depositor
    Masahito Ikawa
    Article
    Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103
    Insert
    split EGFP
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Feb. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    50716-plasmid-map-sequence-id-447281

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  4. AAVS1-SAGFP

    Plasmid
    #127100
    Purpose
    SA-GFP substrate (Addgene #41594) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. SA-GFP cassette contains PGK-puromycin.
    Depositor
    Adam Karpf
    Insert
    SA-GFP reporter
    Expression
    Mammalian
    Available Since
    June 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    127100-plasmid-map-sequence-id-247555

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  9. PEA1-Nuclease

    Plasmid
    #176528
    Purpose
    Delivers all prime editing nuclease components in a single plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9-RT, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    GC to CA point mutation in SpCas9 to restore nucl…
    Promoter
    CbH for Cas9, hU6 for gRNAs
    Available Since
    Dec. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176528-plasmid-map-sequence-id-350557

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  12. LHZ1493

    Plasmid
    #222102
    Purpose
    Cloning backbone for sgRNA in Kluyveromyces marxianus. Expresses highly specific SlugCas9.
    Depositor
    Yongming Wang
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    222102-plasmid-map-sequence-id-441240

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  13. SeqCBE

    Plasmid
    #232115
    Purpose
    Expresses AncBE4max, nSeqCas9 (D10A), and puromycin resistance genes
    Depositor
    Yongming Wang
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Feb. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    232115-plasmid-map-sequence-id-462531

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  14. SeqABE

    Plasmid
    #232114
    Purpose
    Expresses ABEmax, nSeqCas9 (D10A), and puromycin resistance genes
    Depositor
    Yongming Wang
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Feb. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    232114-plasmid-map-sequence-id-462530

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  15. pCMV-T7-TadCBEd-SpRY-P2A-EGFP (BKS428)

    Plasmid
    #223125
    Purpose
    pCMV and pT7 Human expression plasmid for TadCBEd-SpRY with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadCBEd-SpRY-2xUGI-P2A-EGFP
    Use
    CRISPR
    Tags
    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd
    Expression
    Mammalian
    Mutation
    TadA-CDd mutations; nSpRY=D10A/A61R/L1111R/D1135L…
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223125-plasmid-map-sequence-id-447703

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  16. pTB106-Phleo

    Plasmid
    #236780
    Purpose
    Expression of Cas9 cytosine base editor (CBE), T7 RNAP, Cas12a and phleomycin selection marker. The CBE contains a ssDNA-DBD from the L. major RAD51 protein. This plasmid is transfected as episome.
    Depositor
    Tom Beneke
    Article
    Herrmann May et al eLife. 2025 Feb 24;13:RP97437. doi: 10.7
    Insert
    hyBE4max (CBE); Cas12a (Acidaminococcus sp. BV3L6)-P2A-T7 RNAP
    Use
    CRISPR
    Available Since
    April 6, 2026
    Availability
    Academic Institutions and Nonprofits only
    236780-plasmid-map-sequence-id-505276

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  17. pTB107-Hyg

    Plasmid
    #236781
    Purpose
    Expression of Cas9 cytosine base editor (CBE), T7 RNAP, Cas12a and hygromycin selection marker. The CBE contains a ssDNA-DBD from the H. sapiens RAD51 protein. This plasmid is transfected as episome.
    Depositor
    Tom Beneke
    Article
    Herrmann May et al eLife. 2025 Feb 24;13:RP97437. doi: 10.7
    Insert
    hyBE4max (CBE); Cas12a (Acidaminococcus sp. BV3L6)-P2A-T7 RNAP
    Use
    CRISPR
    Available Since
    April 6, 2026
    Availability
    Academic Institutions and Nonprofits only
    236781-plasmid-map-sequence-id-505275

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  18. pTB106-Hyg

    Plasmid
    #236779
    Purpose
    Expression of Cas9 cytosine base editor (CBE), T7 RNAP, Cas12a and hygromycin selection marker. The CBE contains a ssDNA-DBD from the L. major RAD51 protein. This plasmid is transfected as episome.
    Depositor
    Tom Beneke
    Article
    Herrmann May et al eLife. 2025 Feb 24;13:RP97437. doi: 10.7
    Insert
    hyBE4max (CBE); Cas12a (Acidaminococcus sp. BV3L6)-P2A-T7 RNAP
    Use
    CRISPR
    Available Since
    Dec. 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    236779-plasmid-map-sequence-id-494138

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  19. pTB107-Phleo

    Plasmid
    #236782
    Purpose
    Expression of Cas9 cytosine base editor (CBE), T7 RNAP, Cas12a and phleomycin selection marker. The CBE contains a ssDNA-DBD from the H. sapiens RAD51 protein. This plasmid is transfected as episome.
    Depositor
    Tom Beneke
    Article
    Herrmann May et al eLife. 2025 Feb 24;13:RP97437. doi: 10.7
    Insert
    hyBE4max (CBE); Cas12a (Acidaminococcus sp. BV3L6)-P2A-T7 RNAP
    Use
    CRISPR
    Available Since
    Dec. 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    236782-plasmid-map-sequence-id-494137

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Showing: 5061 - 5080 of 6024 results