We narrowed to 5,725 results for: chia

1. J23101

   Plasmid

   #55176

   Purpose

   Very weak constitutive promoter

   Depositor

   Thorsten Mascher

   Article

   Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

   Insert

   J23101 promoter
   

   Use

   Synthetic Biology; Bacillus biobrick box

   Tags

   Expression

   Mutation

   Promoter

   J23101

   Available since

   July 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. BirA_BVES untagged

   Plasmid

   #196581

   Purpose

   expresses BirA

   Depositor

   Cheryl Arrowsmith

   Article

   Alteen et al Structure. 2023 Sep 7;31(9):1121-1131.e6

   Insert

   BirA_Ecoli
   

   Use

   Baculovirus expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 9, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pET28b – E. coli RecE residues 564-868

   Plasmid

   #104534

   Purpose

   Expresses E. coli RecE residues 564-868

   Depositor

   Charles Bell

   Article

   Zhang et al Structure. 2009 May 13;17(5):690-702. do

   Insert

   RecE residues 564-866 (C-terminal nuclease domain)
   

   Use

   Tags

   6X-His tag followed by thrombin site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET41a iGluf

   Plasmid

   #119833

   Purpose

   Production of recombinant iGluSnFR E25D fast variant, a single-wavelength glutamate indicator, in E. coli

   Depositor

   Katalin Torok

   Article

   Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

   Insert

   iGluf
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   E25D

   Promoter

   T7

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIBA2-SpyTag-MBP

   Plasmid

   #107422

   Purpose

   Control construct for SpyCatcher experiments. Encodes MBP with N-terminal SpyTag, including OmpA signal peptide for periplasmic targeting.

   Depositor

   Jack Leo

   Article

   Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/

   Insert

   Maltose-binding protein (MalE)
   

   Use

   Tags

   OmpA signal peptide, SpyTag, and StrepII

   Expression

   Bacterial

   Mutation

   Promoter

   Tet

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSET FLIPglu-600uDelta11 Aphrodite

   Plasmid

   #13569

   Depositor

   Wolf Frommer

   Article

   Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

   Insert

   FLIPglu F16A Delta11 Aphrodite
   

   Use

   Tags

   Aphrodite and ECFP

   Expression

   Bacterial

   Mutation

   Fluorophore-derived linker shortened. F16A mutati…

   Promoter

   Available since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTS2353_Tier3(PB)-YPet_PuroR

   Plasmid

   #169652

   Purpose

   Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a YPet marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PRPBSA-driven YPet and PuroR expression
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PRPBSA

   Available since

   July 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJEC626

   Plasmid

   #174367

   Purpose

   Activation domain for modular CRISPRa. AD-SYNZIP. Expresses alphaNTD from the E coli RNAP with a SYNZIP17 domain fused to the N terminus.

   Depositor

   James Chappell

   Article

   Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

   Insert

   Alpha N-terminal domain from the E. coli RNA polymerase fused to SYNZIP17
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23106

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTYF-1xGfaABC1D-tTA

   Plasmid

   #19977

   Depositor

   Sergey Kasparov

   Article

   Liu et al J Gene Med. 2008 May . 10(5):583-92.

   Insert

   GfaABC1D-tTA (GFAP E.coli, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET30b GluBP600n

    Plasmid

    #119835

    Purpose

    Production of recombinant GluBP600n high affinity protein in E. coli

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    GluBP600n
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET41a iGlul

    Plasmid

    #119832

    Purpose

    Production of recombinant iGluSnFR T92A low affinity variant, a single-wavelength glutamate indicator, in E. coli

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGlul
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    T92A

    Promoter

    T7

    Available since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMM1162

    Plasmid

    #112535

    Purpose

    pZE2-hrtR-RBS3-chuA - LuxCDABE transcriptional unit deletion of plasmid pMM643, ColE1 origin, KanR

    Depositor

    Timothy Lu

    Article

    Mimee et al Science. 2018 May 25;360(6391):915-918.

    Inserts

    HrtR
    ChuA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23107 and ProD

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHL 651

    Plasmid

    #53016

    Purpose

    LacIq + PCon::RBS (st3) tetR::T1 terminator + p15a + CamR. Backbone from pZA32MCS1 (Lutz and Bujard, 1997).

    Depositor

    Han Lim

    Article

    Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

    Inserts

    LacIq
    tetR
    

    Use

    Synthetic Biology

    Tags

    RBS(st3) and T1 terminator

    Expression

    Bacterial

    Mutation

    Promoter

    pCon

    Available since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pRSET FLIPglu-600uDelta11 Ares-Aphrodite

    Plasmid

    #13571

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

    Insert

    FLIPglu F16A Delta11 Aphrodite
    

    Use

    Tags

    Aphrodite and Ares

    Expression

    Bacterial

    Mutation

    Fluorophore-derived linker shortened. F16A mutati…

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET22b-ecDHFR-G51PEKN

    Plasmid

    #118582

    Purpose

    Bacterial expression of E.coli DHFR G51PEKN mutant

    Depositor

    Stephen Benkovic

    Article

    Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

    Insert

    DHFR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G51 mutated to P51; Glu-Lys-Asn residues inserted…

    Promoter

    T7

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET27b-ecDHFR-N23PP/S148A

    Plasmid

    #110829

    Purpose

    Bacterial expression of E.coli DHFR N23PP/S148A mutant

    Depositor

    Stephen Benkovic

    Article

    Bhabha et al Science. 2011 Apr 8;332(6026):234-8. doi

    Insert

    ecDHFR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N23 mutated to P23; a proline residue inserted be…

    Promoter

    T7

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET15b-GB

    Plasmid

    #129689

    Purpose

    express E. coli GreB

    Depositor

    Jeff Gelles

    Article

    Tetone et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

    Insert

    his6/thrombin/GreB (greB E. coli)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRSET FLIPglu-600uDelta13 Aphrodite

    Plasmid

    #13570

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

    Insert

    FLIPglu F16A Delta13 Aphrodite
    

    Use

    Tags

    Aphrodite and ECFP

    Expression

    Bacterial

    Mutation

    Fluorophore-derived linker shortened. F16A mutati…

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDGE76

    Plasmid

    #80578

    Purpose

    recipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, nptII

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Inserts

    pnos:nptII-tmos
    p2x35S:Cas9 D10A-tnos
    BsaI-ccdB_CmR-BsaI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A

    Promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLaNGo11-3

    Plasmid

    #126054

    Purpose

    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Inserts

    chloroplastic FNR transit peptide
    ccdB cassette (BsaI)
    GFP11x3 (5 aa linker)
    

    Use

    Binary

    Tags

    GFP1-10

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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