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Plasmid#167159PurposeGolden Gate entry vector to express the 5th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAtU3Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only
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DT035A
Plasmid#159397PurposeConstitutive superfolder GFP (sfGFP) expression, controlled under E.coli tac promoterDepositorInsertpr.tac-sfGFP
UseSynthetic BiologyTagsFLAGExpressionBacterialMutationPromotertac promoterAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDropVn
Plasmid#184873PurposeChromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for Vibrio natriegens genome editing protocol.DepositorInsertlacZalpha,ccdB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYPQ146-ZmUbi-tRNA
Plasmid#158404PurposeGateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ136-tRNA2.0; assembly of 6 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterMaize ubiquitin 1Available sinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ136B2.0
Plasmid#167160PurposeGolden Gate entry vector to express the 6th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAtU3Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ144-ZmUbi-tRNA
Plasmid#158402PurposeGateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ134-tRNA2.0; assembly of 4 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterMaize ubiquitin 1Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
p221z-CAS9p-t35s
Plasmid#118385PurposeEntry clone containing CAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway systemDepositorInsertCAS9p
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pME-eSIBR
Plasmid#71665Purposeintronically embedded eSIBR backbone in gateway middle entry vector (pDONR221)DepositorInsertintronic eSIBR cassette
UseRNAi; Multi-site gatewayTagsExpressionMutationPromoterNoneAvailable sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
C125-E01: stuffer
Plasmid#162900PurposeStandard Gateway entry clone containing a non-transcribed/translated region for use in generating negative control vectorsDepositorInsertstuffer fragment for vector controls
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pME-Fv2E-flt4um200-ns
Plasmid#67696Purposemiddle entry plasmid with dimerizable extracellular domain fused to um200 mutant Danio Flt4 cytoplasmic domainDepositorInsertFv2E-flt4um200
UseTagsHAExpressionMutationum200, no stop codon; N1056SPromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCZGY3163
Plasmid#101248PurposeGFP11::rps-18, ribosomal protein subunit, bound to C-terminal part of GFP. Shows GFP localized to ribosomes when combined with GFP 1-10.DepositorInsertgfp11::rps-18 (rps-18 Nematode, Synthetic)
UseGatewayTagsGFP11ExpressionMutationPromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAd PdxI-I-nGFP
Plasmid#19411DepositorInsertpancreatic and duodenal homeobox 1 (Pdx1 Mouse)
UseAdenoviral; Entry vectorTagsGFP and IRESExpressionMutationPromoterAvailable sinceNov. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pYPQ154
Plasmid#69305PurposeGateway entry vector with AteCas9 (Arabidopsis codon optimized)DepositorInsertAteCas9 (Arabidopsis codon optimized)
UseCRISPRTagsExpressionMutationNLS fusionPromoterAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEO100c
Plasmid#50550PurposeExpresses sfGFP under the PompC1157 promoterDepositorInsertsfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
p221z-dCAS9p-t35s
Plasmid#118387PurposeEntry clone containing dCAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway systemDepositorInsertdCAS9p
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pME GFP-PCNA
Plasmid#105977Purposeentry clone for gateway cloning, cell cycle marker proliferative cell nuclear antigen PCNADepositorInsertPCNA (PCNA Human)
UseTagsGFPExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCBLsgRNA1
Plasmid#199727PurposeGolden Gate entry vector; 1st gRNA under OsU3 promoterDepositorInsertOsU3-sgRNA1 scaffold
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCBLsgRNA2
Plasmid#199728PurposeGolden Gate entry vector; 2nd gRNA under OsU3 promoterDepositorInsertOsU3-sgRNA2 scaffold
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
p5E-mcs2
Plasmid#138362PurposeGateway compatible 5' entry clone contains mutli-cloning sites sequence2.DepositorInsertsequence multi cloning sites
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFTK020
Plasmid#171292PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertPalcA syn_NoCrea (ANIA_08979) promoter
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only