We narrowed to 31,412 results for: PLE;

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pLenti gRNA KO OCLNx2 spCas9-T2A-iRFP670-P2A-puro

Plasmid

#208399

Purpose

codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, iRFP670 fluorescent protein and puromycin resistance

Depositor

Raphael Gaudin

Article

Pradel et al Autophagy. 2024 Aug;20(8):1825-1836. doi

Insert

hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)

Use

CRISPR and Lentiviral

Tags

iRFP670

Expression

Mammalian

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti gRNA KO OCLNx2 spCas9-T2A-Crimson-P2A-puro

Plasmid

#208398

Purpose

codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, E2-Crimson fluorescent protein and puromycin resistance

Depositor

Raphael Gaudin

Article

Pradel et al Autophagy. 2024 Aug;20(8):1825-1836. doi

Insert

hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)

Use

CRISPR and Lentiviral

Tags

E2-Crimson

Expression

Mammalian

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti gRNA KO OCLNx2 spCas9-T2A-mNeonGreen-P2A-puro

Plasmid

#208400

Purpose

codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, mNeonGreen fluorescent protein and puromycin resistance

Depositor

Raphael Gaudin

Article

Pradel et al Autophagy. 2024 Aug;20(8):1825-1836. doi

Insert

hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)

Use

CRISPR and Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-Myr-Pink Flamindo-p2a-EGFP-bPAC

Plasmid

#202717

Purpose

Mammalian co-expression of membrane-bound Pink Flamindo and soluble bPAC for cAMP perturbation and imaging

Depositor

Adam Cohen

Article

Xiang et al bioRxiv 2023

Inserts

Myr-Pink Flamindo
EGFP-bPAC

Use

Lentiviral

Tags

10xHis and myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-dog-Caspase-1/4a-triple mutant-H342D_K345M_K379T

Plasmid

#183364

Purpose

Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-1/4a (CASP4 Canis lupus familiaris)

Use

Retroviral

Tags

Myc

Mutation

H342D_K345M_K379T

Promoter

MESV

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pCFJ2301 - [1-2] ENTR - Fluor - ce-mMaple3(1 intron, syntrons(3), no_atg, no_stop)

Plasmid

#159865

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-mMaple3(1 intron, syntrons(3), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon1-11E.12D-14.47M-48-3xFLAG

Plasmid

#130684

Purpose

Expresses human SMCHD1 Exon1-11E.12D-14.47M-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon1-11E.12D-14.47M-48 (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

D453_F489del, E653_G1960del

Promoter

CMV

Available Since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon1-10A.11D-14.47M-48-3xFLAG

Plasmid

#130683

Purpose

Expresses human SMCHD1 Exon1-10A.11D-14.47M-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon1-10A.11D-14.47M-48 (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

D409_E452del, E653_G1960del

Promoter

CMV

Available Since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_008-TetO-NLS-hfCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

Plasmid

#223173

Purpose

Expresses hfCas13d (N2V8) in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus.

Depositor

Neville Sanjana

Article

Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

Insert

hfCas13d (N2V8)

Use

Lentiviral

Tags

NLS-HA

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_007 - TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

Plasmid

#138149

Purpose

Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

CasRx

Use

Lentiviral

Tags

NLS-HA

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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