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pNIC28-SPRTN-UBZ (452-489), C456AC459A

Plasmid

#253401

Purpose

Expression in E. coli of SPRTN-UBZ domain carrying the C456AC459A double mutation.

Depositor

Kristijan Ramadan

Article

Song et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

SPRTN (SPRTN Human)

Tags

His

Expression

Bacterial

Mutation

C456AC459A

Availability

Academic Institutions and Nonprofits only

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pNIC28-SPRTN core (26-240)-E112Q

Plasmid

#253403

Purpose

Expression in E. coli of SPRTN 26-240 truncation, carrying the E112Q mutation.

Depositor

Kristijan Ramadan

Article

Song et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

SPRTN (SPRTN Human)

Tags

His

Expression

Bacterial

Mutation

E112Q

Availability

Academic Institutions and Nonprofits only

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pNIC28-SPRTN core (26-240)-L38A

Plasmid

#253404

Purpose

Expression in E. coli of SPRTN 26-240 truncation, carrying the L38A mutation.

Depositor

Kristijan Ramadan

Article

Song et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

SPRTN (SPRTN Human)

Tags

His

Expression

Bacterial

Mutation

L38A

Availability

Academic Institutions and Nonprofits only

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pNIC28-SPRTN core (26-240)-L99A

Plasmid

#253405

Purpose

Expression in E. coli of SPRTN 26-240 truncation, carrying the L99A mutation.

Depositor

Kristijan Ramadan

Article

Song et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

SPRTN (SPRTN Human)

Tags

His

Expression

Bacterial

Mutation

L99A

Availability

Academic Institutions and Nonprofits only

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pGEX-6P1-SPRTN-ZBD-BR (151-245)

Plasmid

#253406

Purpose

Expression in E. coli of SPRTN-ZBD-BR truncation.

Depositor

Kristijan Ramadan

Article

Song et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

SPRTN (SPRTN Human)

Tags

GST

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

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pCold I-SPRTN-PIP-UBZ (320-489)

Plasmid

#253407

Purpose

Expression in E. coli of SPRTN-PIP-UBZ truncation.

Depositor

Kristijan Ramadan

Article

Song et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

SPRTN (SPRTN Human)

Tags

His

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

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MG1655 Z1 malE

Bacterial Strain

#65915

Purpose

E. coli MG1655 Z1 host that produces constitutive levels of TetR, LacI and AraC off of the chromosome with additional malE KO

Depositor

Keith Tyo

Article

Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

Bacterial Resistance

None

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-PE7

Plasmid

#214812

Purpose

Mammalian expression of SpCas9 PE7 prime editor

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pT7-PE7 for IVT

Plasmid

#214813

Purpose

Template for in vitro transcription of PE7

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7

Use

Template for in vitro transcription

Tags

SV40 bpNLS and c-Myc NLS

Promoter

T7 (inactivated)

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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3xFlag-eGFP-Flag-SETX

Plasmid

#218838

Purpose

Mammalian expression of GFP-tagged SETX construct for subcellular localization studies with GFP, or immunoprecipitation with Flag tag.

Depositor

Albert La Spada

Article

Bennett et al Heliyon. 2020 Jun 13;6(6):e04165. doi: 1

Insert

Senataxin (SETX Human)

Tags

Flag and eGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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