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GIV-FLAG S245D (SD)

Plasmid

#86096

Purpose

Expression GIV in mammalian cell

Depositor

Pradipta Ghosh

Article

Aznar et al Elife. 2016 Nov 4;5. pii: e20795. doi: 1

Insert

GIV/Girdin (CCDC88A Human)

Tags

3xFlag

Expression

Mammalian

Mutation

S245D

Promoter

CMV

Available Since

Feb. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot9-H58AF60AA64YV71Y-shRNA1res_W

Plasmid

#147902

Purpose

Mammalian Expression of HsNot9-H58AF60AA64YV71Y-shRNA1res

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot9-H58AF60AA64YV71Y-shRNA1res (CNOT9 Human)

Expression

Mammalian

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot9-Y203AR205AH208AR244A-shRNA1res_W

Plasmid

#147907

Purpose

Mammalian Expression of HsNot9-Y203AR205AH208AR244A-shRNA1res

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot9-Y203AR205AH208AR244A-shRNA1res (CNOT9 Human)

Expression

Mammalian

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot9-R205AH208A-shRNA1res_W

Plasmid

#147903

Purpose

Mammalian Expression of HsNot9-R205AH208A-shRNA1res

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot9-R205AH208A-shRNA1res (CNOT9 Human)

Expression

Mammalian

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot9-Y203AR244A-shRNA1res_W

Plasmid

#147908

Purpose

Mammalian Expression of HsNot9-Y203AR244A-shRNA1res

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot9-Y203AR244A-shRNA1res (CNOT9 Human)

Expression

Mammalian

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti CGIP + M-AAT target

Plasmid

#86008

Purpose

lenti reporter plasmid with M-AAT-specific gRNA target-sequence, to be used with tGFP #26864

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Inserts

CAG promoter
M-AAT target

Use

Lentiviral

Mutation

V30M

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pBK1295-AAV-EFSNC-dCjCas9-HP1a(72-177)

Plasmid

#223140

Purpose

Expression of truncated HP1a with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

HP1a (CBX5 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 72-177

Promoter

EF1a

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1305-AAV-EFSNC-dSaCas9-HP1a(72-177)

Plasmid

#223150

Purpose

Expression of truncated HP1a with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

HP1a (CBX5 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 72-177

Promoter

EF1a

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1299-AAV-EFSNC-dCjCas9-MBD1(529-592)

Plasmid

#223144

Purpose

Expression of truncated MBD1 with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

MBD1 (MBD1 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 529-592

Promoter

EF1a

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1309-AAV-EFSNC-dSaCas9-MBD1(529-592)

Plasmid

#223154

Purpose

Expression of truncated MBD1 with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

MBD1 (MBD1 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 529-592

Promoter

EF1a

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pSD139A

Plasmid

#218129

Purpose

Hybrid circuit displaying hsCRBN-W384A,Y386A bait (P22 anchor) and driving expression of luxAB

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Inserts

CRBN (CRBN Human)
luxAB

Tags

scP22

Mutation

W384A, Y386A

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmThor_50-63-DmeIF4Ga_631-638-DmThor_72-83-GB1_AD

Plasmid

#148514

Purpose

Bacterial Expression of DmThor_50-63-DmeIF4Ga_631-638-DmThor_72-83

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

DmThor_50-63-DmeIF4Ga_631-638-DmThor_72-83 (Thor Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmThor_50-63-DmeIF4Ga_631-675-GB1_AD

Plasmid

#148515

Purpose

Bacterial Expression of DmThor_50-63-DmeIF4Ga_631-675

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

DmThor_50-63-DmeIF4Ga_631-675 (Thor Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_1-1717_X

Plasmid

#147982

Purpose

Insect Expression of DmNot1_1-1717

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_1-1717 (Not1 Fly)

Expression

Insect

Mutation

6 silent mutations and two non silent N605S, K759…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_1-1717-K1537SG1562EQ1658A_X

Plasmid

#147986

Purpose

Insect Expression of DmNot1_1-1717-K1537SG1562EQ1658A

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_1-1717-K1537SG1562EQ1658A (Not1 Fly)

Expression

Insect

Mutation

6 silent mutations and two non silent N605S, K759…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_1-1717-R1197AN1203AF1204A_X

Plasmid

#147987

Purpose

Insect Expression of DmNot1_1-1717-R1197AN1203AF1204A

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_1-1717-R1197AN1203AF1204A (Not1 Fly)

Expression

Insect

Mutation

6 silent mutations and two non silent N605S, K759…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_1-1717-RNFKGQtoAAASEA_X

Plasmid

#147988

Purpose

Insect Expression of DmNot1_1-1717-RNFKGQtoAAASEA

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_1-1717-RNFKGQtoAAASEA (Not1 Fly)

Expression

Insect

Mutation

6 silent mutations and two non silent N605S, K759…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_1-1945-dsRNAres_X

Plasmid

#147989

Purpose

Insect Expression of DmNot1_1-1945-dsRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_1-1945-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

8 silent mutations and two non silent N605S, K759…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1-G1562E-dsRNAres_X

Plasmid

#147996

Purpose

Insect Expression of DmNot1-G1562E-dsRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1-G1562E-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

10 silent mutations and two non silent N605S, K75…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1-K1537SG1562EQ1658A-dsRNAres_W

Plasmid

#147876

Purpose

Insect Expression of DmNot1-K1537SG1562EQ1658A-dsRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1-K1537SG1562EQ1658A-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

10 silent mutations and two non silent N605S, K75…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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