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pDV05.2_pEF1a-iRFP720_secreted

Plasmid

#200409

Purpose

Expresses a secreted version of iRFP720 constitutively in mammalian cells

Depositor

James Collins

Publication

Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

iRFP720 (secreted)

Use

Synthetic Biology

Expression

Mammalian

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pSEVA221T7RNAP

Plasmid

#167974

Purpose

Expresses T7RNAP in E. coli

Depositor

Luis Ángel Fernández

Publication

Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

Insert

T7RNAP (T7p07 Synthetic)

Promoter

TetR-Ptet

Available since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pETSG

Plasmid

#159418

Purpose

Expression of proteins with a C-terminal thermostable GFP (TGP) tag in Escherichia coli

Depositor

Dianfan Li

Publication

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

thermostable GFP (TGP)

Tags

polyhistidine (poly-His)

Expression

Bacterial

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pQLinkHD-SYNZIP6

Plasmid

#80662

Purpose

Encodes the synthetic coiled coil peptide SYNZIP6

Depositor

Amy Keating

Publication

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP6

Tags

6xHis

Expression

Bacterial

Available since

March 4, 2019

Availability

Academic Institutions and Nonprofits only

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pQLinkHD-SYNZIP5

Plasmid

#80661

Purpose

Encodes the synthetic coiled coil peptide SYNZIP5

Depositor

Amy Keating

Publication

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP5

Tags

6xHis

Expression

Bacterial

Available since

March 4, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-SYNZIP17

Plasmid

#80671

Purpose

Encodes the synthetic coiled coil peptide SYNZIP17

Depositor

Amy Keating

Publication

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP17

Use

Gateway Cloning

Available since

Nov. 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBSV2G_PflaB-mEGFPBb

Plasmid

#118234

Purpose

Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mEGFP

Depositor

Christine Jacobs-Wagner

Publication

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

flagellin promoter driven mEGFP

Expression

Bacterial

Available since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pBSV2G_PflaB-msfYFPBb

Plasmid

#118233

Purpose

Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven msfYFP

Depositor

Christine Jacobs-Wagner

Publication

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

flagellin promoter driven msfYFP

Expression

Bacterial

Available since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pQLinkHD-SYNZIP4

Plasmid

#80660

Purpose

Encodes the synthetic coiled coil peptide SYNZIP4

Depositor

Amy Keating

Publication

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP4

Tags

6xHis

Expression

Bacterial

Available since

Feb. 2, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMB72

Plasmid

#73088

Purpose

C-terminal GFP-2xTEV-Avi vector, for fosmid recombineering

Depositor

Mike Boxem

Publication

Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

Insert

GFP-Avi tag

Tags

GFP and Avi

Expression

Worm

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pGS775

Plasmid

#20169

Depositor

Stephen West

Publication

Dunderdale et al J Biol Chem. 1994 Feb 18. 269(7):5187-94

Insert

RuvC

Expression

Bacterial

Mutation

none

Available since

April 1, 2009

Availability

Academic Institutions and Nonprofits only
pEM184_30x147bp_Widom601

Plasmid

#240634

Purpose

30x 147-bp Widom 601 nucleosome positioning sequence

Depositor

Emma Morrison

Publication

Hammonds et al bioRxiv [Preprint]. 2025 Apr 10:2025.04.

Insert

30x 147-bp Widom 601

Use

Dna amplification

Available since

Sept. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pSLQ14101 aeBlue

Plasmid

#239453

Purpose

express aeBlue chromoprotein

Depositor

Stanley Qi

Publication

Collins et al Nat Commun. 2024 Aug 3;15(1):6563. doi:

Insert

aeBlue

Use

CRISPR; Cell-free system using bacterial extract

Expression

Bacterial

Promoter

J23119

Available since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAG306-pTet07-nLuc

Plasmid

#199755

Purpose

Control construct of elongation reporter to quantify elongation of YFP

Depositor

Brian Zid

Publication

Hou et al RNA. 2023 Dec;29(12):1928-1938. doi: 10.

Insert

Nanoluc

Expression

Yeast

Promoter

TetO7

Available since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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HT109_pAAV_hSyn-DiO-SomQuasAr6a_EGFP

Plasmid

#190878

Purpose

Cre-on expression of soma-targeted QuasAr6a under an neuronal promoter

Depositor

Adam Cohen

Publication

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

somQuasAr6a_EGFP

Use

AAV

Tags

EGFP

Promoter

hSyn

Available since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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HT028_FCMV_CheRiff_EGFP

Plasmid

#178821

Purpose

Expresses a blue-light activated cationic channelrhodopsin CheRiff carrying an EGFP tag

Depositor

Adam Cohen

Publication

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

CheRIff_EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-3xFLAG-WPRE

Plasmid

#127862

Purpose

pAAV plasmid expressing 3xFLAG under the hSyn promoter

Depositor

Florian Freudenberg

Publication

Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

Insert

3xFLAG

Use

AAV

Promoter

hSyn

Available since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pV1081

Plasmid

#111425

Purpose

Solo vector for expression of CaCas9 and sgRNA for CaADE2 mutagenesis - integrates at ENO1

Depositor

Gerald Fink

Publication

Vyas et al Sci Adv. 2015;1(3):e1500248.

Insert

CaCas9/sgADE-NT2

Use

CRISPR

Expression

Yeast

Available since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pOGG001

Plasmid

#113978

Purpose

Level 0 module. nptII promoter for constitutive gene expression

Depositor

Philip Poole

Publication

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

nptII promoter

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-ncpGCaMP6s

Plasmid

#113674

Purpose

A topological variant of GCaMP6s

Depositor

Robert Campbell

Publication

Qian et al Chembiochem. 2018 Jun 22. doi: 10.1002/c

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ncpGCaMP6s

Expression

Mammalian

Promoter

CMV

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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