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pT3TS-spCas9 ABE-Umax-ex2-rest1

Plasmid

#222143

Purpose

Adenine base editor with modified TadA variant for extended window and restricted A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Publication

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

Modified TadA-nSpCas9

Use

CRISPR; Zebrafish expression

Tags

Bipartite NLS

Promoter

T3

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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PCDNA3-PAR2-GFP11-T2A-GFP10-bArrestin1-T2A mCherry

Plasmid

#113609

Purpose

PPI reporter

Depositor

Xiaokun Shu

Publication

Zhang et al Protein Sci. 2018 Apr;27(4):874-879. doi

Insert

PAR2

Expression

Mammalian

Available since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pT3TS-spCas9 ABE-Umax-ex1-rest1

Plasmid

#222140

Purpose

Adenine base editor with modified TadA variant for extended window and restricted A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Publication

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

Modified TadA-nSpCas9

Use

CRISPR; Zebrafish expression

Tags

Bipartite NLS

Promoter

T3

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV syn Arrestin-TEVp-V5

Plasmid

#173118

Purpose

Expresses arrestin receiver construct in neurons

Depositor

Alice Ting

Publication

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

Arrestin-TEVp-V5

Use

AAV

Promoter

Synapsin

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-G4DBD-CREST (C91)

Plasmid

#22963

Depositor

Anirvan Ghosh

Insert

CREST (Ss18l1 Rat)

Tags

G4DBD

Expression

Mammalian

Available since

Jan. 29, 2010

Availability

Academic Institutions and Nonprofits only
PCDNA3-S1P1-GFP11-T2A-GFP10-bArrestin1-T2A mCherry

Plasmid

#113612

Purpose

PPI reporter

Depositor

Xiaokun Shu

Publication

Zhang et al Protein Sci. 2018 Apr;27(4):874-879. doi

Insert

S1P1

Expression

Mammalian

Available since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pET E. coli expression vector with BioBrick polypromoter restriction sites (14-A)

Plasmid

#48307

Depositor

Scott Gradia

Type

Empty backbone

Expression

Bacterial

Available since

Nov. 5, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pGS-M1-BRG1-3xFLAG-BAF57-BAF60A

Plasmid

#109190

Purpose

Plasmid for expression of C-terminally 3xFLAG-tagged BRG1, untagged BAF57 and untagged BAF60A under the control of a p10 promoter in baculovirus/insect cell systems

Depositor

Monika Golas

Publication

Jayaprakash et al Mol Cell Biochem. 2019 Nov;461(1-2):171-

Inserts

BRG1 (SMARCA4 Human)
BAF57 (SMARCE1 Human)
BAF60A (SMARCD1 Human)

Tags

3xFLAG

Expression

Insect

Promoter

p10

Available since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-hisSumo-KE-Des27.7_noCoreStab

Plasmid

#248014

Purpose

Expression of synthetic Kemp eliminase (Ablation of Des27.7 ModularAssembly&PROSS&ActiveSite stability)

Depositor

Sarel Fleishman

Publication

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.7_noCoreStab

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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zCREST3:Cas9green; T2_gRNA

Plasmid

#197647

Purpose

pDEL160; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 type II sgRNA

Depositor

William Talbot

Publication

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

zCREST3
Cas9
nrg1 type II sgRNA

Use

Tol2 destination vector

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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zCREST3:Cas9green; T1_gRNA

Plasmid

#199332

Purpose

pDEL159; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 type I sgRNA

Depositor

William Talbot

Publication

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

zCREST3
Cas9
nrg1 type I sgRNA

Use

Tol2 destination vector

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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JGE302_pAAV-hSyn-DIO-Myc-PYLcs-bArrestin2-YFP (p2)

Plasmid

#107247

Purpose

beta arrestin 2 fused to PYLcs for abscisic acid mediated recruitment of ABIcs labelled proteins.

Depositor

Bryan Roth

Publication

Roth et al bioRxiv 251769

Insert

ARRB2

Use

AAV

Tags

MYC, PYLcs, and YFP

Promoter

hSYN

Available since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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pET LIC cloning vector with BioBrick polycistronic restriction sites (9A)

Plasmid

#48283

Depositor

Scott Gradia

Type

Empty backbone

Expression

Bacterial

Available since

Nov. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pPB UbC Arrestin-uTEV2-V5; UAS-mCherry

Plasmid

#173123

Purpose

Expresses uTEV2 and reporter receiver construct in DIPG

Depositor

Alice Ting

Publication

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

Arrestin-uTEV2-V5; UAS-mCherry

Expression

Mammalian

Promoter

UbC

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac LIC cloning vector with BioBrick polycistronic restriction sites (11A)

Plasmid

#48294

Depositor

Scott Gradia

Publication

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Expression

Insect

Available since

Nov. 5, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET His6 TEV expression vector with BioBrick polypromoter restriction sites (14-B)

Plasmid

#48308

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6

Expression

Bacterial

Available since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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Beta-arrestin2 K11,12R GFP

Plasmid

#35412

Depositor

Robert Lefkowitz

Publication

Shenoy et al J Biol Chem. 2005 Apr 15;280(15):15315-2

Insert

βarrestin2 (Arrb2 Rat)

Tags

EGFP

Expression

Mammalian

Mutation

K11,12R

Promoter

CMV

Available since

March 16, 2012

Availability

Academic Institutions and Nonprofits only
pGS-M1-BRG1-3xFLAG

Plasmid

#109188

Purpose

Plasmid for expression of C-terminally 3xFLAG-tagged BRG1 under the control of a p10 promoter in baculovirus/insect cell systems

Depositor

Monika Golas

Publication

Jayaprakash et al Mol Cell Biochem. 2019 Nov;461(1-2):171-

Insert

BRG1 (SMARCA4 Human)

Tags

3xFLAG

Expression

Insect

Promoter

p10

Available since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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Myc-CREST (C88)

Plasmid

#22960

Depositor

Anirvan Ghosh

Insert

CREST (Ss18l1 Rat)

Tags

Myc

Expression

Mammalian

Available since

Jan. 29, 2010

Availability

Academic Institutions and Nonprofits only
FLAG-β-arrestin2 RRK/Q-5-kinase domain fusion protein (RRK5K)

Plasmid

#38262

Depositor

Robert Lefkowitz

Publication

Nelson et al J Biol Chem. 2008 Jul 25;283(30):21093-1

Insert

β-arrestin2 (Arrb2 Rat)

Tags

Flag and PIP5K Iα core kinase domain (residues 18…

Expression

Mammalian

Mutation

Arg 233, Arg237, and Lys251 converted to Glutamin…

Promoter

CMV

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

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