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pGWB525

Plasmid

#74867

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with T7 (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pCS2z-tal1

Plasmid

#164656

Purpose

for the synthesis of zebrafish tal1 mRNA

Depositor

Didier Stainier

Article

Mattonet et al Sci Adv. 2022 Sep 2;8(35):eabn2082. doi:

Insert

tal1 (tal1 Zebrafish)

Use

Mrna synthesis

Promoter

SP6

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pGWB429

Plasmid

#74823

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with TAP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pCC20

Plasmid

#198783

Purpose

Drives specific expression in the ASK neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Psra-9-GAL4-SK(DBD)-VP64

Expression

Worm

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pGWB526

Plasmid

#74868

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with T7 (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

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pMM1157

Plasmid

#112534

Purpose

pZE2-PLhrtO-lux-hrtR-RBS3 - ChuA transciptional unit deletion of plasmid pMM643, ColE1 origin, KanR

Depositor

Timothy Lu

Article

Mimee et al Science. 2018 May 25;360(6391):915-918.

Inserts

HrtR
luxCDABE

Expression

Bacterial

Promoter

PL(HrtO) and ProD

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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823 pGEX2T PTEN G129R

Plasmid

#10749

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

PTEN (PTEN Human)

Tags

GST

Expression

Bacterial

Mutation

G129R

Available Since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only
pLV_mU6-sgNGN3_hU6-sgRNA_hUbC-PuroR-P2A-GFP

Plasmid

#162336

Purpose

Lentiviral expression of sgNGN3 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

S. pyogenes sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

hU6; mU6

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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VRER variant

Plasmid

#138561

Purpose

Expresses human codon-optimized SpCas9-VRER and blasticidin resistance: EFS promoter-VRER-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

VRER

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

D1135V, G1218R, R1335E, T1337R

Promoter

EFS

Available Since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCI-Neo-gp78/2853

Plasmid

#11435

Depositor

Allan Weissman

Article

Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

Insert

gp78 (AMFR Human)

Expression

Mammalian

Mutation

n/a

Available Since

April 5, 2006

Availability

Academic Institutions and Nonprofits only

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pGWB427

Plasmid

#74821

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with T7 (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Sept. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB426

Plasmid

#74820

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with T7 (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pPBpuro-mNG-eDHFR(69K6)-Tiam1

Plasmid

#172102

Purpose

Mammalian expression of Tiam1(DH-PH) fused to mNeonGreen-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

mNeonGreen-eDHFR(69K6)-Tiam1(DH-PH) (TIAM1 Human)

Expression

Mammalian

Promoter

CAG

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pGWB422

Plasmid

#74816

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with GST (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB428

Plasmid

#74822

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with TAP (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

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pMM549

Plasmid

#112531

Purpose

pZA1D-hrtR-chuA - HrtR expressed consitutively from promoter ProD with RBS2, ChuA expressed consitiutively from promoter J23107, p15a origin, AmpR

Depositor

Timothy Lu

Article

Mimee et al Science. 2018 May 25;360(6391):915-918.

Inserts

HrtR
ChuA

Expression

Bacterial

Promoter

J23107 and ProD

Available Since

Feb. 15, 2020

Availability

Academic Institutions and Nonprofits only

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p53-(1-393)-E285A-N268D

Plasmid

#35090

Depositor

Cheryl Arrowsmith

Article

Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Contains aa 1-393 - E285A-N268D

Promoter

T7

Available Since

April 4, 2012

Availability

Academic Institutions and Nonprofits only
pDONR223 PAK5 T538N

Plasmid

#60537

Purpose

Donor vector for human Pak5 T538N mutant

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

Pak5 (PAK5 Human)

Use

Gateway Cloning

Mutation

T538N

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pDONR223 PAK5 S573N

Plasmid

#60538

Purpose

Donor vector for human Pak5 S573N mutant

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

Pak5 (PAK5 Human)

Use

Gateway Cloning

Mutation

S573N

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pScalps_puro_mklf1

Plasmid

#59918

Purpose

Expresses mouse Kruppel-like factor1

Depositor

Silvia Monticelli

Article

Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

Insert

Kruppel-like factor 1 (Klf1 Mouse)

Use

Lentiviral

Available Since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only