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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 5161 - 5180 of 78592 results

  1. MuHKII-pGFPN3

    Plasmid
    #21922
    Depositor
    Hossein Ardehali
    Publication
    Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Mutant huamn HKII (with a mutation in the catalytic site in the C-terminal half of the enzyme) in pGFP-N3 (HK2 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    This is the full length human HKII cDNA sequence …
    Available since
    Oct. 14, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    21922-plasmid-map-sequence-id-520511

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  3. 803 pGEX2T PTEN

    Plasmid
    #10784
    Depositor
    William Sellers
    Insert
    PTEN (PTEN Human)
    Tags
    GST
    Expression
    Bacterial
    Available since
    Nov. 14, 2005
    Availability
    Academic Institutions and Nonprofits only

  4. 803 pGEX2T PTEN

    Plasmid
    #10784
    Depositor
    William Sellers
    Insert
    PTEN (PTEN Human)
    Tags
    GST
    Expression
    Bacterial
    Available since
    Nov. 14, 2005
    Availability
    Academic Institutions and Nonprofits only

  10. pEM705 Gst (Cys)

    Plasmid
    #109376
    Purpose
    GsX chimera with Gαt C-terminus (Pertussis toxin sensitive)
    Depositor
    Robert Lucas
    Publication
    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Gst chimera
    Tags
    None
    Expression
    Mammalian
    Mutation
    Cys at position 391 (Pertussis Toxin Sensitive)
    Promoter
    CAG
    Available since
    May 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    109376-plasmid-map-sequence-id-206235

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  13. pCMV-PE6f

    Plasmid
    #207856
    Purpose
    Mammalian expression of PE6f prime editor
    Depositor
    David Liu
    Publication
    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE6f
    Tags
    SV40 bpNLS and SV40 bpNLS, c-Myc NLS
    Expression
    Mammalian
    Mutation
    Cas9(H840A, E471K, H99R, I632V, H721Y, D645N, K91…
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    207856-plasmid-map-sequence-id-408168

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  14. pCMV-PE6g

    Plasmid
    #207857
    Purpose
    Mammalian expression of PE6g prime editor
    Depositor
    David Liu
    Publication
    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE6g
    Tags
    SV40 bpNLS and SV40 bpNLS, c-Myc NLS
    Expression
    Mammalian
    Mutation
    Cas9(H840A, E471K, H99R, I632V, H721Y, R654C, D64…
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    207857-plasmid-map-sequence-id-408164

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  16. lentiCRISPR_DKO

    Plasmid
    #183192
    Purpose
    Plasmid with Cas9, two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen
    Depositor
    Lang Li
    Publication
    Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    hU6, mU6
    Available since
    Aug. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    183192-plasmid-map-sequence-id-360098

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  17. pYPQ-dzCas9-Act3.0

    Plasmid
    #158414
    Purpose
    CRISPR-Act3.0 system containing dzCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.
    Depositor
    Yiping Qi
    Publication
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Insert
    dzCas9-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available since
    July 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    158414-plasmid-map-sequence-id-332091

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  18. pMasterK

    Plasmid
    #165081
    Purpose
    Episomal expression of reprogramming factors.
    Depositor
    Hideyuki Okano
    Publication
    Yoshimatsu et al Stem Cell Res. 2021 May;53:102380. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    OCT4 (POU5F1 Human)
    SOX2 (SOX2 Human)
    KLF4 (KLF4 Human)
    MYC (MYC Human)
    LRH1 (NR5A2 Human)
    NANOG (NANOG Human)
    lin28 (LIN28A Human)
    EGFP
    HSV TK
    KDM4D (KDM4D Callithrix jacchus)
    GLIS1 (GLIS1 Human)
    Use
    Episomal
    Expression
    Mammalian
    Available since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    165081-plasmid-map-sequence-id-514763

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  19. pAAV-Ef1a-Con/Fon-oScarlet (AAV8)

    Viral prep
    #137136-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Con/Fon-oScarlet (#137136). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Fon-oScarlet plasmid DNA. EF1a-driven, Cre and Flp-dependent expression of oScarlet. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Publication
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    EF1a
    Tags
    oScarlet (Cre and Flp-dependent)
    Available since
    April 5, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 5161 - 5180 of 78592 results