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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,132 results for: pcDNA 3.1

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Showing: 5161 - 5180 of 6132 results

  6. Cenp-B DBD INCENP TSS/AAA mCherry

    Plasmid
    #45234
    Purpose
    Target INCENP (with TSS mutated to AAA) to centromeres via CENP-B DNA binding domain (DBD)
    Depositor
    Michael Lampson
    Article
    Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.
    Insert
    Cenp-B DBD, INCENP-ΔCEN with AAA mutation, mCherry
    Tags
    CENP-B DBD, DNA binding domain of CENP-B and mChe…
    Expression
    Mammalian
    Mutation
    INCENP C-terminal TSS mutated to AAA
    Promoter
    t7
    Available Since
    Sept. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    45234-plasmid-map-sequence-id-84485

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  7. Antares-SEPLuc

    Plasmid
    #141086
    Purpose
    precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis
    Depositor
    Jeak Ling Ding
    Article
    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41
    Insert
    Antares-T2A-puromycin-EMCV IRES-SEPLuc
    Expression
    Mammalian
    Mutation
    Deletion of amino acids 66 to 91 on the Luciferas…
    Promoter
    CMV
    Available Since
    June 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    141086-plasmid-map-sequence-id-296862

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  9. Opto-FFR3

    Plasmid
    #106065
    Purpose
    Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-FFR3; Opto-FFR3; F7; 718)
    Depositor
    Harald Janovjak
    Article
    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:
    Insert
    Opto-FFR3
    Tags
    Rhodopsin-1D4 and VSV-G
    Expression
    Mammalian
    Mutation
    Chimeric receptor protein
    Promoter
    CMV
    Available Since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    106065-plasmid-map-sequence-id-209706

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  14. TVMV-HRV-3C Proteases-mCherry

    Plasmid
    #243796
    Purpose
    Encodes for mammalian expression of TVMV and HRV-3C protease inputs. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.
    Depositor
    Cole DeForest
    Article
    Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/
    Insert
    TVMV and HRV-3C proteases
    Expression
    Mammalian
    Available Since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    243796-plasmid-map-sequence-id-489527

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  15. TVMV-TEV Proteases-mCherry

    Plasmid
    #243797
    Purpose
    Encodes for mammalian expression of TVMV and TEV protease inputs. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.
    Depositor
    Cole DeForest
    Article
    Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/
    Insert
    TVMV and TEV proteases
    Expression
    Mammalian
    Available Since
    Oct. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    243797-plasmid-map-sequence-id-486448

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  16. ACE2-Mutant-C4

    Plasmid
    #240608
    Purpose
    To test ACE2 cleavage by TMPRSS2. This protease targets R and K amino acids of ACE2, so these amino acids were substituted by A at Cluster 4 located within the collectrin-like site on ACE2
    Depositor
    Zaid Abassi
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    FLAG tag
    Expression
    Mammalian
    Mutation
    Arginine (R) and Lysine (K) residues on ACE2-clus…
    Promoter
    CMV
    Available Since
    Sept. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    240608-plasmid-map-sequence-id-483854

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  17. ACE2-Mutant-C(3+4)

    Plasmid
    #240611
    Purpose
    To test ACE2 cleavage by TMPRSS2. This protease targets R and K amino acids of ACE2, so these amino acids were substituted by A at Cluster 3 and Cluster 4 of ACE2
    Depositor
    Zaid Abassi
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    FLAG tag
    Expression
    Mammalian
    Mutation
    Arginine (R) and Lysine (K) residues on both ACE2…
    Promoter
    CMV
    Available Since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    240611-plasmid-map-sequence-id-482833

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  18. ACE2-Mutant-C(0+4)

    Plasmid
    #240610
    Purpose
    To test ACE2 cleavage by TMPRSS2. This protease targets R and K amino acids of ACE2, so these amino acids were substituted by A at Cluster 0 and Cluster 4 of ACE2
    Depositor
    Zaid Abassi
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    FLAG tag
    Expression
    Mammalian
    Mutation
    Arginine (R) and Lysine (K) residues on both ACE2…
    Promoter
    CMV
    Available Since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    240610-plasmid-map-sequence-id-482820

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Showing: 5161 - 5180 of 6132 results