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pRetro-CAG-DsRedExpress-TVA

Plasmid

#235699

Purpose

Control plasmid expressing TVA and DsRed without G protein

Depositor

Guo-li Ming, Hongjun Song

Article

Sun et al Nature. 2025 Jan 16. doi: 10.1038/s41586

Insert

DsRedExpress, TVA800

Use

Retroviral

Promoter

CAG

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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HA-APP-mGFP

Plasmid

#196696

Purpose

Expresses APP695 with an HA tag an N-terminal for single molecule tracking and other measusers in mammalian cells

Depositor

Martino Calamai

Article

Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

Insert

Amyloid Precursor Protein 695 isoform (APP Human)

Tags

HA and mGFP

Expression

Mammalian

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pBABE-hygro-2xMyc-Rac1-P29S

Plasmid

#128581

Purpose

For mammalian cell expression of P29S mutant murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.

Depositor

Julian Downward

Article

Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

Insert

Rac1 (Rac1 Mouse)

Use

Retroviral

Tags

2xMyc epitope

Expression

Mammalian

Mutation

Changed Proline29 to Serine.

Available Since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE-hygro-2xMyc-Rac1-Q61L

Plasmid

#128582

Purpose

For mammalian cell expression of Q61L mutant murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.

Depositor

Julian Downward

Article

Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

Insert

Rac1 (Rac1 Mouse)

Use

Retroviral

Tags

2xMyc epitope

Expression

Mammalian

Mutation

Changed Glutamine61 to Leucine.

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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hKCNQ5(WT).ires.GFP-pcDNA3

Plasmid

#204360

Purpose

Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

Depositor

Aguan Wei

Article

Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

Insert

KCNQ5 (KCNQ5 Human)

Expression

Mammalian

Promoter

CMV/T7 RNApol

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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HA-BACE1-mBFP

Plasmid

#196697

Purpose

Expresses Bace1 with an HA tag at N-terminal for single molecule tracking and other measusers in mammalian cells

Depositor

Martino Calamai

Article

Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

Insert

Beta-secretase 1 (BACE1 Human)

Tags

HA and mBFP

Expression

Mammalian

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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hKCNQ3(WT).ires.mCherry-pcDNA3

Plasmid

#204362

Purpose

Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

Depositor

Aguan Wei

Article

Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

Insert

KCNQ3 (KCNQ3 Human)

Expression

Mammalian

Promoter

CMV/T7 RNApol

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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hKCNQ5(WT).ires.mScarlet-pcDNA3

Plasmid

#204361

Purpose

Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

Depositor

Aguan Wei

Article

Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

Insert

KCNQ5 (KCNQ5 Human)

Expression

Mammalian

Promoter

CMV/T7 RNApol

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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hBACE1(D289N).ires.mCherry-pcDNA3

Plasmid

#204363

Purpose

Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

Depositor

Aguan Wei

Article

Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

Insert

BACE1 (BACE1 Human)

Expression

Mammalian

Mutation

D289N

Promoter

CMV/T7 RNApol

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC39A8_STOP

Plasmid

#161343

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC39A8 (SLC39A8 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CAG-PM-NGN3-linker-3xFLAG-Dendra2-HA

Plasmid

#234888

Purpose

Expresses human phosphomutant-NGN3 in mammalian cells directly fused to Dendra2 fluorescent protin plus FLAG and HA tags

Depositor

Nancy Papalopulu

Article

Miller et al Dev Cell. 2025 Sep 22;60(18):2518-2532.e

Insert

Phosphomutant-NGN3-3xFLAG-Dendra2-HA (NEUROG3 Human)

Tags

3xFLAG, Dendra2 and HA

Expression

Mammalian

Mutation

Changed Serines 161, 165, 174, 183 and 191 to Ala…

Promoter

CAG

Available Since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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hKCNQ5(K289N).ires.GFP-pcDNA3

Plasmid

#204364

Purpose

Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

Depositor

Aguan Wei

Article

Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

Insert

KCNQ5 (KCNQ5 Human)

Expression

Mammalian

Mutation

K289N

Promoter

CMV/T7 RNApol

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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hKCNQ5(P369T).ires.GFP-pcDNA3

Plasmid

#204365

Purpose

Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

Depositor

Aguan Wei

Article

Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

Insert

KCNQ5 (KCNQ5 Human)

Expression

Mammalian

Mutation

P369T

Promoter

CMV/T7 RNApol

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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hKCNQ5(R443Q).ires.GFP-pcDNA3

Plasmid

#204366

Purpose

Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

Depositor

Aguan Wei

Article

Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

Insert

KCNQ5 (KCNQ5 Human)

Expression

Mammalian

Mutation

R443Q

Promoter

CMV/T7 RNApol

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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hKCNQ5(H576R).ires.GFP-pcDNA3

Plasmid

#204367

Purpose

Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

Depositor

Aguan Wei

Article

Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

Insert

KCNQ5 (KCNQ5 Human)

Expression

Mammalian

Mutation

H576R

Promoter

CMV/T7 RNApol

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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hKCNQ5(V145G).ires.GFP-pcDNA3

Plasmid

#204368

Purpose

Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

Depositor

Aguan Wei

Article

Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

Insert

KCNQ5 (KCNQ5 Human)

Expression

Mammalian

Mutation

V145G

Promoter

CMV/T7 RNApol

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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hKCNQ5(L341I).ires.GFP-pcDNA3

Plasmid

#204369

Purpose

Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

Depositor

Aguan Wei

Article

Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

Insert

KCNQ5 (KCNQ5 Human)

Expression

Mammalian

Mutation

L341I

Promoter

CMV/T7 RNApol

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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hKCNQ5(P369R).ires.GFP-pcDNA3

Plasmid

#204370

Purpose

Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

Depositor

Aguan Wei

Article

Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

Insert

KCNQ5 (KCNQ5 Human)

Expression

Mammalian

Mutation

P369R

Promoter

CMV/T7 RNApol

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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hKCNQ5(S448I).ires.GFP-pcDNA3

Plasmid

#204371

Purpose

Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

Depositor

Aguan Wei

Article

Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

Insert

KCNQ5 (KCNQ5 Human)

Expression

Mammalian

Mutation

S448I

Promoter

CMV/T7 RNApol

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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hKCNQ5(R178X).ires.GFP-pcDNA3

Plasmid

#204372

Purpose

Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

Depositor

Aguan Wei

Article

Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

Insert

KCNQ5 (KCNQ5 Human)

Expression

Mammalian

Mutation

R178X

Promoter

CMV/T7 RNApol

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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