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Showing: 501 - 520 of 873 results

  1. pW210-pPB-GA-inducible-CRISPRa-sa_dCas9-VPR-hygR

    Plasmid
    #170808
    Purpose
    PiggyBac vector to express GA-inducible VPR-Sa_dCas9 with hygromycin resistance
    Depositor
    Kenneth Yamada
    Article
    Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.
    Inserts
    GID1-VPR-IRES-HygR
    GAI-tagBFP-Sa_dCas9 (GAI Synthetic, S. aureus, Mustard Weed)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Synonymous mutation in PYL1 to remove XhoI site (…
    Promoter
    Available since
    June 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    170808-plasmid-map-sequence-id-334406

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  2. P25-COMP-blac-flag-his

    Plasmid
    #110991
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    ookinete surface protein P25 (PF10_0303 Synthetic)
    Use
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    110991-plasmid-map-sequence-id-224481

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  3. ADH1pro_GCR1_SIR4

    Plasmid
    #138618
    Purpose
    Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement
    Depositor
    Rob Mitra
    Insert
    GCR1 (GCR1 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Available since
    March 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    138618-plasmid-map-sequence-id-274926

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  4. pDONR221-olig1

    Plasmid
    #192744
    Purpose
    Gateway entry vector encoding zebrafish olig1
    Depositor
    Summer Thyme
    Insert
    olig1 (olig1 Zebrafish)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    F106L
    Promoter
    None
    Available since
    Nov. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    192744-plasmid-map-sequence-id-377802

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  6. PHF20 (3Q1J)

    Plasmid
    #28278
    Depositor
    Cheryl Arrowsmith
    Insert
    PHF20 (PHF20 Human)
    Use
    Tags
    His with TEV cleavage
    Expression
    Bacterial
    Mutation
    residues 1-81.
    Promoter
    Available since
    April 14, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. 4TVR

    Plasmid
    #61952
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    UBH14 (UHRF2 Human)
    Use
    Tags
    MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    March 2, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  9. pGWB14-LYX140

    Plasmid
    #126358
    Purpose
    With a Nicotiana benthamiana transcription factor in PGWB14 backbone, for Agrobacterium mediated transient or stable expression corresponding transcription factor fused with a C-terminal HA tag.
    Depositor
    Silin Zhong
    Insert
    Niben101Scf10260g00003.1
    Use
    Tags
    HA
    Expression
    Plant
    Mutation
    Promoter
    CaMV 35S
    Available since
    June 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    126358-plasmid-map-sequence-id-247494

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  10. pGWB14-LYX138

    Plasmid
    #126356
    Purpose
    With a Nicotiana benthamiana transcription factor in PGWB14 backbone, for Agrobacterium mediated transient or stable expression corresponding transcription factor fused with a C-terminal HA tag.
    Depositor
    Silin Zhong
    Insert
    Niben101Scf10560g00003.1
    Use
    Tags
    HA
    Expression
    Plant
    Mutation
    Promoter
    CaMV 35S
    Available since
    June 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    126356-plasmid-map-sequence-id-247488

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  11. pGWB14-LYX119

    Plasmid
    #126337
    Purpose
    With a Nicotiana benthamiana transcription factor in PGWB14 backbone, for Agrobacterium mediated transient or stable expression corresponding transcription factor fused with a C-terminal HA tag.
    Depositor
    Silin Zhong
    Insert
    Niben101Scf10232g00003.1
    Use
    Tags
    HA
    Expression
    Plant
    Mutation
    Promoter
    CaMV 35S
    Available since
    June 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    126337-plasmid-map-sequence-id-247266

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  12. pGWB14-LYX090

    Plasmid
    #126308
    Purpose
    With a Nicotiana benthamiana transcription factor in PGWB14 backbone, for Agrobacterium mediated transient or stable expression corresponding transcription factor fused with a C-terminal HA tag.
    Depositor
    Silin Zhong
    Insert
    Niben101Scf10062g00008.1
    Use
    Tags
    HA
    Expression
    Plant
    Mutation
    Promoter
    CaMV 35S
    Available since
    June 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    126308-plasmid-map-sequence-id-246969

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  13. pGWB14-LYX094

    Plasmid
    #126312
    Purpose
    With a Nicotiana benthamiana transcription factor in PGWB14 backbone, for Agrobacterium mediated transient or stable expression corresponding transcription factor fused with a C-terminal HA tag.
    Depositor
    Silin Zhong
    Insert
    Niben101Scf10889g01018.1
    Use
    Tags
    HA
    Expression
    Plant
    Mutation
    Promoter
    CaMV 35S
    Available since
    June 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    126312-plasmid-map-sequence-id-246976

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  14. pGWB14-LYX026

    Plasmid
    #126244
    Purpose
    With a Nicotiana benthamiana transcription factor in PGWB14 backbone, for Agrobacterium mediated transient or stable expression corresponding transcription factor fused with a C-terminal HA tag.
    Depositor
    Silin Zhong
    Insert
    Niben101Scf10283g01004.1
    Use
    Tags
    HA
    Expression
    Plant
    Mutation
    Promoter
    CaMV 35S
    Available since
    June 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    126244-plasmid-map-sequence-id-246442

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  15. UBH14

    Plasmid
    #28158
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    E3 ligase UHRF2 (UHRF2 Human)
    Use
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    SRA Domain (residues 419-648)
    Promoter
    Available since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  16. PPI64

    Plasmid
    #25137
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ppi64.001.179 (PPIG Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    K125A/E126A
    Promoter
    Available since
    Aug. 5, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  17. UBH12 (3CLZ)

    Plasmid
    #25220
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ubh12.414.617:B9 (UHRF1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 12, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. pCMV6-XL4 FLAG-LRRC3-Fc

    Plasmid
    #115766
    Purpose
    Expression of the extracellular domain of LRRC3 with N-terminal FLAG tag and C-terminal hIgG tag.
    Depositor
    Davide Comoletti
    Insert
    LRRC3 (LRRC3 Human)
    Use
    Tags
    FLAG and hIgG/Fc
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    Oct. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    115766-plasmid-map-sequence-id-223835

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  19. UBH12 (3DB3)

    Plasmid
    #25164
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ubh12.126.285 (UHRF1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 6, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. CMYA5tnC/pBSSK+

    Plasmid
    #98119
    Purpose
    CMYA5 (NM_153610, nt 11648 to 12279) in pBSSK+ (minimum binding region to titin)
    Depositor
    Rita Shiang
    Insert
    CMYA5 (CMYA5 Human)
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 18, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    98119-plasmid-map-sequence-id-191012

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Showing: 501 - 520 of 873 results