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Addgene

We narrowed to 6,521 results for: poly

Showing: 501 - 520 of 6521 results
  1. pSN-A112C-1A32

    Plasmid
    #191241
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A32 and a His-tag, for fluorescent studies.
    Depositor
    Insert
    nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    Use
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available since
    Feb. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
  2. pSN-A112C-1A31

    Plasmid
    #191242
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A31 and a His-tag, for fluorescent studies.
    Depositor
    Insert
    nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    Use
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available since
    Jan. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
  3. pSN-A112C-CP8B1

    Plasmid
    #191240
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-CP8B1 and a His-tag, for fluorescent studies.
    Depositor
    Insert
    nuc (nuclease A), CYP8B1 (CYP12) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    Use
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
  4. CRY2olig-PR

    Plasmid
    #218917
    Purpose
    Expression of CRY2olig-mCherry fused to a codon-optimised poly-PR sequence
    Insert
    CRY2olig-mCherry tagged poly-PR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    wild-type
    Promoter
    CMV
    Available since
    Aug. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. CRY2olig-GP

    Plasmid
    #218918
    Purpose
    Expression of CRY2olig-mCherry fused to a codon-optimised poly-GP sequence
    Insert
    CRY2olig-mCherry tagged poly-GP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    wild-type
    Promoter
    CMV
    Available since
    Aug. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. CRY2olig-GR

    Plasmid
    #218919
    Purpose
    Expression of CRY2olig-mCherry fused to a codon-optimised poly-GR sequence
    Insert
    CRY2olig-mCherry tagged poly-GR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    wild-type
    Promoter
    CMV
    Available since
    Aug. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
  7. pMW-PahZ1 KT-1

    Plasmid
    #141134
    Purpose
    Expresses in BL-21 (DE3) cells to produce the poly(aspartic acid) hydrolase-1 from Sphingomonas Sp. KT-1 which selectively cleaves the beta linkages in thermally synthesized poly(aspartic acid).
    Depositor
    Insert
    PahZ1
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Not the full-length gene. PahZ1 fragment starts a…
    Promoter
    Available since
    Sept. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
  8. pAG426GAL-SLEV_NS2B-GS-NS3

    Plasmid
    #203510
    Purpose
    Expresses SLEV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 marker
    Depositor
    Insert
    poly SLEV NS2B-GS-NS3 (POLY )
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Available since
    July 27, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 501 - 520 of 6521 results