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pCMV Sport2 mPer2

Plasmid

#16204

Depositor

Cheng Lee

Article

Albrecht et al Cell. 1997 Dec 26. 91(7):1055-64.

Insert

Per2 (Per2 Mouse)

Expression

Mammalian

Available Since

Dec. 6, 2007

Availability

Academic Institutions and Nonprofits only
evoBE4-NG

Plasmid

#226852

Purpose

Expresses evoBE4max-NG base editor in mammalian cells with eGFP marker

Depositor

Alexis Komor

Article

Osgood et al Methods in Enzymology, February 5, 2025

Insert

evoBE4max-NG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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Synaptojanin2-pmCherryC1

Plasmid

#27677

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Synaptojanin 2 (Synj2 Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only
ABE8.20-NG

Plasmid

#226857

Purpose

Expresses ABE8.20-NG base editor in mammalian cells with eGFP marker

Depositor

Alexis Komor

Article

Osgood et al Methods in Enzymology, February 5, 2025

Insert

ABE8.20-NG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCLAP

Plasmid

#200786

Purpose

Cloning template for N-terminal CLIPf tagging and C-terminal SNAPf tagging of constructs for mammalian expression. Also used for the mammalian expression of CLIPf-SNAPf-HisTag-FLAG fusion protein.

Depositor

Alison Twelvetrees

Article

Smith et al bioRxiv 2024.12.20.629623

Type

Empty backbone

Tags

CLIPf and SNAPf-6xHis-FLAG

Expression

Mammalian

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-Backbone(NG)

Plasmid

#27963

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TAL-Backbone(NG)

Use

Lentiviral

Expression

Mammalian

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pSFV3-lacZ

Plasmid

#92074

Purpose

This is an expression plasmid for production of recombinant genomic SFV RNA encoding beta-galactosidase.

Depositor

Henrik Garoff

Article

Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356

Insert

lacZ

Use

Lacz expression plasmid, for run-off transcriptio…

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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CALM-pmCherryN1

Plasmid

#27691

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

CALM (Picalm Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

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pKH3-human RSK1

Plasmid

#13841

Depositor

John Blenis

Article

Richards et al Mol Cell Biol. 2001 Nov . 21(21):7470-80

Insert

RSK1 (RPS6KA1 Human)

Tags

3x HA

Expression

Mammalian

Available Since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only

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ancBE4-NG

Plasmid

#226853

Purpose

Expresses ancBE4max-NG base editor in mammalian cells with eGFP marker

Depositor

Alexis Komor

Article

Osgood et al Methods in Enzymology, February 5, 2025

Insert

ancBE4max-NG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCD/NL-BH*DDD

Plasmid

#17531

Depositor

Jakob Reiser

Article

Zhang et al J Virol. 2004 Feb . 78(3):1219-29.

Insert

HIV-1 Gag/Pol, Tat, Rev

Use

Lentiviral

Expression

Mammalian

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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pShuttle-Cre-HA

Plasmid

#16583

Depositor

Bert Vogelstein

Article

Jallepalli et al Cell. 2001 May 18. 105(4):445-57.

Insert

cre recombinase (cre P1 bacteriophage)

Use

Adenoviral

Tags

HA

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3-dCry-V5His

Plasmid

#136241

Purpose

Protein expression of D. melanogaster (Dm) cytochrome with V5 and His tags

Depositor

Aziz Sancar

Article

Ozturk et al Biochemistry. 2009 Sep 15;48(36):8585-93

Insert

DmCry

Tags

His and V5

Expression

Mammalian

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pGL2Basic-EcadK1

Plasmid

#19290

Depositor

Eric Fearon

Article

Hajra et al Oncogene. 1999 Dec 2. 18(51):7274-9.

Insert

E-cadherin

Use

Luciferase

Expression

Mammalian

Mutation

E-cad promoter sequences from -108 to +125

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only

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pBABE HAII

Plasmid

#14738

Depositor

Adrienne Cox

Article

Fiordalisi et al Methods Enzymol. 2001;332:3-36. doi: 10.

Type

Empty backbone

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Aug. 29, 2007

Availability

Academic Institutions and Nonprofits only

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pCAGGS-NICD

Plasmid

#26891

Depositor

Nicholas Gaiano

Article

Dang et al Dev Neurosci. 2006;28(1-2):58-69.

Insert

NICD (Notch1 Mouse)

Tags

FLAG and b-globin polyA

Expression

Mammalian

Mutation

E2299K

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
YFP-tagged FRB (YR)

Plasmid

#20148

Depositor

Tobias Meyer

Article

Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

Insert

YFP-FRB (YR)

Expression

Mammalian

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only

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pMPRA1-CMV-Clover

Plasmid

#53538

Purpose

Fluorescent reporter control for pMPRA1

Depositor

Tarjei Mikkelsen

Article

Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

Insert

Clover GFP

Expression

Mammalian

Promoter

CMV IE

Available Since

May 20, 2014

Availability

Academic Institutions and Nonprofits only

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pMPRA2

Plasmid

#49350

Purpose

Backbone for transient transfection of Massively Parallel Reporter Assays (MPRA) libraries, pPGK-H2Kk for physical selection of transfected cells

Depositor

Tarjei Mikkelsen

Article

Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pBabePuro-bcl2

Plasmid

#21144

Depositor

Junying Yuan

Article

Miura et al Cell. 1993 Nov 19. 75(4):653-60.

Insert

pBabePuro-bcl 2

Use

Retroviral

Expression

Mammalian

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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