We narrowed to 10,032 results for: hwa

1. pRevTRE MKL1-N100

   Plasmid

   #19848

   Depositor

   Ron Prywes

   Article

   Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

   Insert

   MKL1-N100 (MRTFA Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mutation

   deleted amino acids 1-100

   Promoter

   Available since

   Jan. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

2. pCDNA3 Flag MKK4(ala)

   Plasmid

   #15314

   Depositor

   Roger Davis

   Article

   Whitmarsh et al Science. 1995 Jul 21. 269(5222):403-7.

   Insert

   MKK4(ala) (MAP2K4 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Ser-220Ala Thr-224Ala

   Promoter

   Available since

   July 25, 2007

   Availability

   Academic Institutions and Nonprofits only

3. 3341 pcDNA3 Bcl-2 T69A S70A S87A

   Plasmid

   #13340

   Depositor

   Stanley Korsmeyer

   Article

   Yamamoto et al Mol Cell Biol 1999 Dec;19(12):8469-78.

   Insert

   Bcl-2 (BCL2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed Threonine 69 to Alanine; changed Serine 7…

   Promoter

   Available since

   Nov. 9, 2006

   Availability

   Academic Institutions and Nonprofits only

4. TTR C10S S85C

   Plasmid

   #184410

   Purpose

   bacterial expression of TTR

   Depositor

   Peter Wright

   Article

   Sun et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

   Insert

   TTR (TTR Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C10S, S85C

   Promoter

   T7

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPAICS-2XStrep

   Plasmid

   #125683

   Purpose

   expresses human PAICS protein with a C-terminal 2XStrep affinity tag

   Depositor

   Stephen Benkovic

   Article

   Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

   Insert

   PAICS (PAICS Human)
   

   Use

   Tags

   2X Strep-Tag II

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. YF-Cdc42(CA)

   Plasmid

   #20152

   Depositor

   Tobias Meyer

   Article

   Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

   Insert

   YFP-FKBP-Cdc42(CA) (CDC42 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Cdc42H was inserted into the multiple cloning sit…

   Promoter

   Available since

   March 26, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRK5-FLAG-Sestrin3

   Plasmid

   #72592

   Purpose

   overexpression, codon optimized for mammalian expression

   Depositor

   David Sabatini

   Article

   Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

   Insert

   SESN3 (SESN3 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   codon optimized for mammalian expression, see dep…

   Promoter

   CMV

   Available since

   March 28, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pCMV5 MEKK2 WT

   Plasmid

   #12182

   Depositor

   Gary Johnson

   Article

   Blank et al J Biol Chem. 1996 Mar 8. 271(10):5361-8.

   Insert

   MEKK2 (Map3k2 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 22, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pCMV-flag Mst2 (KR)

   Plasmid

   #27372

   Depositor

   Kunliang Guan

   Article

   Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

   Insert

   Mst2 (STK3 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   K56R (catalytically inactive)

   Promoter

   Available since

   Feb. 2, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pRK5-Flag CASTOR1 I280A

    Plasmid

    #84495

    Purpose

    overexpression of CASTOR1 I280A mutant

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

    Insert

    CASTOR1 I280A (GATSL3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    I280A

    Promoter

    CMV

    Available since

    Oct. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pLXSN-H-Ras_N17

    Plasmid

    #39537

    Depositor

    Julian Downward

    Article

    Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

    Insert

    HRAS N17 (HRAS Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    N17

    Promoter

    Available since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pCMV5 MEKK4alpha WT

    Plasmid

    #12187

    Depositor

    Gary Johnson

    Article

    Gerwins et al J Biol Chem. 1997 Mar 28. 272(13):8288-9

    Insert

    MEKK4 (Map3k4 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

13. SYMPK

    Plasmid

    #156071

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    SYMPK (SYMPK Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair4

    Plasmid

    #98975

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 94bp 3' overhangs. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNAs pair 4 and Cas9(D10A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNAIIIB cdc42 QL

    Plasmid

    #61636

    Purpose

    mammalian expression of cdc42 constitutively active mutant

    Depositor

    JS Gutkind

    Article

    Coso et al Cell. 1995 Jun 30;81(7):1137-46.

    Insert

    Cdc42 (CDC42 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q61L, constitutively active

    Promoter

    CMV

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pRW004-SM-destination-proUBQ10-Cas9

    Plasmid

    #104438

    Purpose

    Destination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;

    Depositor

    Detlef Weigel

    Article

    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    UBQ10

    Available since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET23-PBD(65-150)-N-Cys

    Plasmid

    #13722

    Depositor

    Klaus Hahn

    Article

    Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

    Insert

    PBD(65-150)-N-Cys (PAK1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Met-Cys-Gly-PBD(65-150)-

    Promoter

    Available since

    March 28, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pWZL Hygro-NFLAG TRF1 deltaA deltaM

    Plasmid

    #16297

    Depositor

    Titia de Lange

    Article

    Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

    Insert

    hTRF1 (TERF1 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Deleted acidic domain (aa1-66) and myb domain (aa…

    Promoter

    Available since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pTET2-RlucCP

    Plasmid

    #198352

    Purpose

    Part of Firefly reporter system, encodes Renilla luciferase

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    Renilla Luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pTET2

    Available since

    April 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. PRPF18

    Plasmid

    #155846

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    PRPF18 (PRPF18 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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