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pGEX-4T1_GST-GBRP

Plasmid

#223728

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

GABARAP (GABARAP Human)

Tags

GST

Expression

Bacterial

Mutation

aa 2-116 only, with Glycine exposed

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-CD59

Plasmid

#222307

Purpose

Synchronize the trafficking of CD59 from the ER.

Depositor

Franck Perez

Article

Fourriere et al J Cell Biol. 2019 Jul 1;218(7):2215-2231

Insert

Streptavidin-KDEL and CD59 fused to SBP-EGFP (CD59 Human)

Expression

Mammalian

Mutation

signal peptide removed

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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CA245 CMVTO_ERret-H2M-Renin_P2A_mRens-hIL6-rens.1_T2A_mRens-cageIL-10-c-myc_T2A-mCherry

Plasmid

#231206

Purpose

Single transcript cageIL-10 (mRens) + cleaveIL-6 (mRens) + ER-renin (H2M)

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

ERret-H2M-Renin_P2A_mRens-hIL6-rens.1_T2A_mRens-cageIL-10-c-myc_T2A-mCherry (IL10 Human)

Expression

Mammalian

Mutation

L147I, R148H, I203V, L290V

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-MARK2-K82H-CR

Plasmid

#229425

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

MARK2-K82H (MARK2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Kinase-dead mutant K82H; silent mutation CRISPR r…

Promoter

EFS

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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FLAG-MARK2-GK-CR

Plasmid

#229423

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

MARK2-GK-CR (MARK2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Gatekeeper mutation M129G; silent mutation CRISPR…

Promoter

EFS

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-HBEGF-mNeonGreen

Plasmid

#209912

Purpose

To monitor the expression of HB-EGF, the plasmid encodes a recombinant HB-EGF fused intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

HBEGF-mNeonGreen (HBEGF Human)

Use

Lentiviral

Tags

mNeonGreen

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-EGR1-WT

Plasmid

#215592

Purpose

For overexpression of Gal4-DBD EGR1 WT

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

EGR1 (EGR1 Human)

Expression

Mammalian

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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PD2529 human beta1 ecto

Plasmid

#221398

Purpose

Mammalian expression of human integrin beta1 ectodomain

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 ectodomain (ITGB1 Human)

Tags

AVI tag, HRV3C cleavage site, basic coil, HA tag,…

Expression

Mammalian

Mutation

codon optimized for human mature _1 residues Q1 t…

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CLIP-CD28(CD45TM)

Plasmid

#223629

Purpose

Lentiviral expression of human CD28 (CD45TM) with CLIP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

CLIP

Mutation

CD28 transmembrane domain replaced by CD45 transm…

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-Spy-CD28(CD45TM)

Plasmid

#223624

Purpose

Lentiviral expression of human CD28 (CD45TM) with SpyTag003 in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

Spytag003

Mutation

CD28 transmembrane domain replaced by CD45 transm…

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-SNAP-CD86(F56A)

Plasmid

#223609

Purpose

Lentiviral expression of human CD86 (F56A) with SNAP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD86 (CD86 Human)

Use

Lentiviral; mammalian expression

Tags

SNAP

Mutation

F56A

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-SNAP-CD80(Y65A)

Plasmid

#223608

Purpose

Lentiviral expression of human CD80 (Y65A) with SNAP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD80 (CD80 Human)

Use

Lentiviral; mammalian expression

Tags

SNAP

Mutation

Y65A

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CLIP-CD28(SGGG)

Plasmid

#223607

Purpose

Lentiviral expression of human CD28 (SGGG) with CLIP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

CLIP

Mutation

MYPPPY mutated to SGGG

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pPPI4-Strep-SNAP-CTLA-4-GCN4-His6

Plasmid

#223582

Purpose

Protein purification of human CTLA4 with Twin-Strep, SNAP, GCN4 and 6xHis tags from mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

Insert

CTLA4 (CTLA4 Human)

Tags

GCN4, His6 and Twin-Strep, SNAP

Expression

Mammalian

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pPPI4-Strep-SNAP-CD80 (I92R)-His10

Plasmid

#223580

Purpose

Protein purification of human CD80 (I92R) with Twin-Strep, SNAP and 10xHis tags from mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

Insert

CD80 (CD80 Human)

Tags

His10 and Twin-Strep, SNAP

Expression

Mammalian

Mutation

I92R

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pPPI4-Strep-SNAP-CD80-His10

Plasmid

#223579

Purpose

Protein purification of human CD80 with Twin-Strep, SNAP and 10xHis tags from mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

Insert

CD80 (CD80 Human)

Tags

His10 and Twin-Strep, SNAP

Expression

Mammalian

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-EGR1-AroSCRAMBLED

Plasmid

#215594

Purpose

For overexpression of Gal4-DBD EGR1 AroSCRAMBLED

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

EGR1 AroSCRAMBLED (EGR1 Human)

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-HHEX

Plasmid

#222928

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of HHEX

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

HHEX (HHEX Human)

Expression

Mammalian

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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Beta1 Q280* K418* full-length

Plasmid

#221406

Purpose

Mammalian expression of human integrin beta1 Q280* K418* full-length

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 Q280* K418* full-length (ITGB1 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

Expression

Mammalian

Mutation

codon-optimized mature sequence

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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Beta1 N391* N562* full-length

Plasmid

#221407

Purpose

Mammalian expression of human integrin beta1 N391* N562* full-length

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 N391* N562* full-length (ITGB1 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

Expression

Mammalian

Mutation

codon-optimized mature sequence

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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