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pME-rox-nls-mCherry-V5-2xStop-rox (JDW 1167)

Plasmid

#224524

Purpose

A Gateway compatible middle entry clone containing a Rox flanked 3xNLS-mCherry-V5-2xStop cassette

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

rox-3xNLS-mCherry-V5-2xSTOP-rox

Use

Gateway Cloning

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pME-3xHA-BirA-T2A-mCherry-caax (JDW 889)

Plasmid

#224512

Purpose

A Gateway compatible middle entry clone containing Biotin Ligase followed by T2A and mCherry membrane reporter (biotinylation and flourescent cell membrane reporter)

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

3xHA-BirA-T2A-mCherry-Caax

Use

Gateway Cloning

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/T0-EGFP-GNB1L

Plasmid

#222621

Purpose

Mammalian expression vector of flag-tagged GNB1L

Depositor

Daniel Durocher

Publication

Zhao et al Mol Cell. 2023 Aug 3;83(15):2792-2809.e9

Insert

GNB1L (GNB1L Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Aug. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-CAG-fDIO-OFF-ArgiNLS-AausFP1

Plasmid

#220605

Purpose

Flp-independent expression of a single-cell discriminating version of AausFP1 fluorescent protein. Flp activity can turn off expression.

Depositor

Sam Golden

Publication

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

AausFP1

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Promoter

CAG

Available since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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Rif1-Halo HRD

Plasmid

#207081

Purpose

Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RIF1 locus.

Depositor

Jens Schmidt

Publication

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

HaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences

Tags

HaloTag

Expression

Mammalian

Promoter

None

Available since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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Halo-MDC1 PST deletion

Plasmid

#207109

Purpose

pHTN HaloTag CMV-neo (Promega; #G7721) based vector to express HaloTag-MDC1 with a deletion of the PST repeat region in human cells.

Depositor

Jens Schmidt

Publication

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

HaloTag-MDC1 PST deletion

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCD86-mEYFP(Q69K)-mEGFP

Plasmid

#190750

Purpose

Expression of CD86 double tagged with mEYFP(Q69K) and mEGFP in mammalian cells.

Depositor

Dave Piston

Publication

Foust et al Biophys J. 2025 Oct 21;124(20):3354-3365

Insert

CD86 (CD86 Human)

Tags

mEGFP and mEYFP(Q69K)

Expression

Mammalian

Promoter

CMV

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-eGFP-OPTN D474N

Plasmid

#175762

Purpose

Lentiviral vector expressing N-terminally tagged eGFP-OPTN D474N

Depositor

Wade Harper

Publication

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

OPTN (OPTN Human)

Use

Lentiviral

Mutation

D474N

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-FLAG-APEX2-GABARAPL2Y49A/L50A

Plasmid

#175752

Purpose

Lentiviral vector expressing N-terminally tagged APEX2-FLAG-GABARAPL2Y49A/L50A

Depositor

Wade Harper

Publication

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

GABARAPL2 (GABARAPL2 Human)

Use

Lentiviral

Mutation

Y49A L50A

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3_ReBphP-PCM_IRES_sfGFP

Plasmid

#166917

Purpose

Express ReBphP-PCM and sfGFP

Depositor

Andre Stiel

Publication

Mishra et al Sci Adv. 2020 Jun 12;6(24):eaaz6293. doi

Inserts

ReBphP-PCM
sfGFP

Expression

Mammalian

Promoter

CMV

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP104-ChromeT-mCherry

Plasmid

#123126

Purpose

AAV-mediated expression of ChromeT-mCherry under the GFAP promoter.

Depositor

Edward Boyden

Publication

Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

Insert

ChromeT-mCherry

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

Channelrhodopsin-2 (ChR2) with mutations A71S/ E9…

Promoter

GFAP104

Available since

April 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-H2-Dd-VN

Plasmid

#135517

Purpose

Mammalian expression of VN-fused and myc-tagged H2-Dd

Depositor

Erik Procko

Publication

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

H2-Dd (H2-D1 Mouse)

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

C121S

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA p21 K163R

Plasmid

#78787

Purpose

To overexpress p21 K163R in Mammalian Cells

Depositor

Jaewhan Song

Publication

Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

Insert

p21 (CDKN1A Human)

Tags

HA

Expression

Mammalian

Mutation

K163R_mutation

Promoter

CMV

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA p21 K161R

Plasmid

#78788

Purpose

To overexpress p21 K161R in Mammalian Cells

Depositor

Jaewhan Song

Publication

Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

Insert

p21 (CDKN1A Human)

Tags

HA

Expression

Mammalian

Mutation

K161R_mutation

Promoter

CMV

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pONL-C1_RhoB

Plasmid

#65698

Purpose

Expresses ONL-RhoB in mammalian cells

Depositor

Yasushi Okada

Publication

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

RhoB (RHOB Human)

Use

Luciferase

Tags

ONL

Expression

Mammalian

Promoter

CMV

Available since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pYNL-C1_RhoB

Plasmid

#65696

Purpose

Expresses YNL-RhoB in mammalian cells

Depositor

Yasushi Okada

Publication

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

RhoB (RHOB Human)

Use

Luciferase

Tags

YNL

Expression

Mammalian

Promoter

CMV

Available since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pYNL-C1_PTS1

Plasmid

#65699

Purpose

Expresses YNL-PTS1 in mammalian cells

Depositor

Yasushi Okada

Publication

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

peroxisomal targeting signal 1 (tripeptide SKL)

Use

Luciferase

Tags

YNL

Expression

Mammalian

Promoter

CMV

Available since

July 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCNL-N1_Golgi

Plasmid

#65694

Purpose

Expresses Golgi-CNL in mammalian cells

Depositor

Yasushi Okada

Publication

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

N-terminal 81 amino acids of beta 1,4-galactosyltransferase

Use

Luciferase

Tags

CNL

Expression

Mammalian

Promoter

CMV

Available since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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pYNL-N1_Golgi

Plasmid

#65693

Purpose

Expresses Golgi-YNL in mammalian cells

Depositor

Yasushi Okada

Publication

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

N-terminal 81 amino acids of beta 1,4-galactosyltransferase

Use

Luciferase

Tags

YNL

Expression

Mammalian

Promoter

CMV

Available since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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pCNL-C1_PTS1

Plasmid

#65700

Purpose

Expresses CNL-PTS1 in mammalian cells

Depositor

Yasushi Okada

Publication

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

peroxisomal targeting signal 1 (tripeptide SKL)

Use

Luciferase

Tags

CNL

Expression

Mammalian

Promoter

CMV

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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