We narrowed to 18,046 results for: Mut

1. gRNA-trp-HYB

   Plasmid

   #64331

   Purpose

   trp1 deletion gRNA cassette carried by pRS42H

   Depositor

   Yong-Su Jin

   Article

   Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

   Insert

   gBlock product of trp1 deletion gRNA cassette
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pMax_mPlum

   Plasmid

   #177830

   Purpose

   Host Cell Reactivation (HCR) control mPlum plasmid (pMax backbone)

   Depositor

   Leona Samson, Zachary Nagel

   Article

   Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

   Insert

   mPlum
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-FLAG-SBP-STAG2 (WT)

   Plasmid

   #73963

   Purpose

   STAG2 cDNA (CCDS43990) wild type

   Depositor

   Todd Waldman

   Article

   Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

   Insert

   STAG2 (STAG2 Human)
   

   Use

   Tags

   FLAG-SBP

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   Available Since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pNIA-CEN-FLAG-LINXa4(RLR)-Bre1

   Plasmid

   #116884

   Purpose

   FLAG-tagged LINXa4-Bre1 with RLR hinge-loop mutations

   Depositor

   Brian Kuhlman

   Article

   Lerner et al ACS Synth Biol. 2018 Nov 29. doi: 10.102

   Insert

   LINXa4(RLR)-Bre1
   

   Use

   Synthetic Biology

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Bre1 point mutations K8A, K9A and K11A, LINXa3 wi…

   Promoter

   ADH1

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti-UbC-FKRP-EF1a-BSD

   Plasmid

   #205150

   Purpose

   Express UbC-driven FKRP and EF-1a driven BSD

   Depositor

   Monkol Lek

   Insert

   Fukutin-related protein (FKRP Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UbC

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Cas9 MDC123

   Plasmid

   #59184

   Purpose

   2x35S promoting Glycing Max codon optimized Cas9 with bar plant selection

   Depositor

   Robert Stupar

   Article

   Michno et al GM Crops Food. 2015;6(4):243-52. doi: 10

   Insert

   Glycine Max codon optimized Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   2X35S

   Available Since

   Nov. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. H1.224.M.luc

   Plasmid

   #193705

   Purpose

   Expresses a truncated PolIII H1 promoter of 224 nucleotides (H1.224) with the TATA box mutated

   Depositor

   Elena Herrera-Carrillo

   Article

   Gao et al J Biol Chem. 2020 Nov 5. pii: S0021-9258

   Insert

   Truncated PolIII H1 promoter (H1.224) with the TATA box mutated
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-sapphireCKAR(T/A)

   Plasmid

   #118469

   Purpose

   Negative-control mutant for sapphireCKAR biosensor.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   sapphireCKAR(T/A)
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Contains Thr-to-Ala mutation in substrate sequenc…

   Promoter

   CMV

   Available Since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGH020_sgRNA_G418-GFP

   Plasmid

   #85405

   Purpose

   hu6 driven sgRNA vector with G418 and GFP selectable markers

   Depositor

   Michael Bassik

   Article

   Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   G418 resistance and GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330-Cetn1/1

    Plasmid

    #50718

    Purpose

    pX330 containing sgRNA against mouse Cent1. Positive control for DSB mediated EGFP reconstitution.

    Depositor

    Masahito Ikawa

    Article

    Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

    Inserts

    Cetn1 sgRNA1
    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and hU6

    Available Since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-STAG2-wildtype

    Plasmid

    #31972

    Depositor

    Todd Waldman

    Article

    Solomon et al Science. 2011 Aug 19;333(6045):1039-43.

    Insert

    human STAG2 cDNA (STAG2 Human)
    

    Use

    Tags

    EGFP and FLAG

    Expression

    Mammalian

    Mutation

    wild-type

    Promoter

    CMV

    Available Since

    Aug. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFYF1548 EMX1

    Plasmid

    #47508

    Purpose

    human gRNA expression vector targeting EMX1

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-EMX1 (EMX1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1(+)-blueAKAR(T/A)

    Plasmid

    #118471

    Purpose

    Negative-control mutant for blueAKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    blueAKAR(T/A)
    

    Use

    Tags

    6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available Since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-Tag4A-MITF-M (wt)

    Plasmid

    #31151

    Depositor

    Yardena Samuels

    Article

    Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

    Insert

    MITF-M (MITF Human)
    

    Use

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    WT

    Promoter

    Available Since

    July 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR221-hSOX10

    Plasmid

    #24749

    Depositor

    William Pavan

    Article

    Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

    Insert

    (sex determining region Y)-box 10 (SOX10 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    wildtype sequence

    Promoter

    Available Since

    May 18, 2010

    Availability

    Academic Institutions and Nonprofits only

16. MBP-TEVcs(ENLYFQ/G)-His6-uTEV1Δ(220-242)-R5

    Plasmid

    #135455

    Purpose

    Bacterial expression of uTEV1Δ protease

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    MBP-TEVcs(ENLYFQ/G)-His6-TEV1Δ(220-242)-R5
    

    Use

    Tags

    MBP, His6

    Expression

    Bacterial

    Mutation

    S219V mutation improves stability, S153N mutation…

    Promoter

    tac promoter

    Available Since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJOG1368

    Plasmid

    #167301

    Purpose

    Level 0 module (CDS1) for further assemblies based on the Modular Cloning system. FCY-UPP gene conferring sensitivity to 5-Fluorocytosine

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Insert

    FCY-UPP fusion gene
    

    Use

    Synthetic Biology; Moclo level 0 vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJOG1336

    Plasmid

    #167303

    Purpose

    Level 0 module (3U+Ter) for further assemblies based on the Modular Cloning system. 3'UTR and flanking sequence of a Solanum lycopersicum Oleosin gene, arbitrarily called OLE2 (Sollyc03g112440)

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Insert

    3'UTR and Terminator SlOLE2
    

    Use

    Synthetic Biology; Moclo level 0 vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJOG1334

    Plasmid

    #167302

    Purpose

    Level 0 module (Pro+5U) for further assemblies based on the Modular Cloning system. Promoter and 5'UTR of a Solanum lycopersicum Oleosin gene, arbitrarily called OLE2 (Sollyc03g112440)

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Insert

    Promoter SlOLE2
    

    Use

    Synthetic Biology; Moclo level 0 vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-AcGFP-progerin

    Plasmid

    #86947

    Purpose

    Construct used to synthesize progerin mRNA. Please note the plasmid contains LMNA gene with nucleotide C1824T mutation.

    Depositor

    Alex Hewitt

    Insert

    AcGFP-progerin (LMNA Human)
    

    Use

    AAV

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    progerin sequence is LMNA gene with nucleotide C1…

    Promoter

    CMV and T7

    Available Since

    June 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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