We narrowed to 7,766 results for: loxP
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Plasmid#206268PurposeDEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes SpCas9 under the control of CMV promoter. CRE-Acceptor in MultiBac system.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSpCas9
UseCRISPR; Recombinant baculovirus productionExpressionMammalianAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-lox-inv[Talpha1-Dre-PA]-lox-N-Cadherin-2xHA
Plasmid#196896PurposeNeuron-specific expression of N-Cadherin fused to 2xHA-tags. Used in combination with Talpha1-iCre-pA plasmidsDepositorAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLAG-IRES-Blasticidin-hOCT4 WT
Plasmid#198764PurposeMammalian expression of hOCT4DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthOCT4 WT (POU5F1 Human)
TagsFLAGExpressionMammalianAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUAS-kkv-mos::NG
Plasmid#138958PurposeUsed to generate a transgene that will express a fluorescent Chitin Synthase in Drosophila where the active site is converted to the mosquito sequence. Expression by using Gal4DepositorPublicationInsertkkv (NEWENTRY Fly)
UseUas transgene vectorTagsNeon Green fused to C terminus of KkvExpressionInsectMutation8 bp that are in the region that encodes the cata…PromoterUASAvailable sinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDB-hCRY1-Luciferase-hvTK-bla
Plasmid#179444PurposeDonor vector to knock in firefly Luciferase C-terminal to human CRY1 geneDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLuciferase
UseCRISPRTagsHis/FlagPromoterNoneAvailable sinceFeb. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgCtrl_EF1a-Puro-T2A-BFP
Plasmid#195500PurposeLentiviral BFP expression vector bearing a sgRNA targeting a randomized human TSS sequenceDepositorInsertsgRNA
UseCRISPR and LentiviralTagsBFP, Puro, and T2AExpressionMammalianAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-Cas9-P2A-Puro_NFATC2-sgRNA
Plasmid#188704PurposeLentivirus transfer plasmid encoding Cas9, puromycin resistance, and 4 sgRNAs targeting human NFATC2DepositorInsertNFATC2 sgRNAs
UseLentiviralExpressionMammalianPromoterhUbCAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ef1a-DIO-somAion-citrine
Plasmid#192573PurposeAAV-vector for optogenetic long-term silencing of Cre-positive neuronsDepositorInsertsomAion
UseAAVTagsCitrine - soma-targeting motif from Kv2.1 channelExpressionMammalianMutationT59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…PromoterEf1-alphaAvailable sinceDec. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLAG-IRES-blasticidin-Sox2 S248A
Plasmid#182047Purposeexpression of Sox2 mutantsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSox2 (Sox2 Mouse)
TagsFLAGExpressionMammalianMutationS248APromoterCAGAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLAG-IRES-blasticidin-Sox2 T258A
Plasmid#182048Purposeexpression of Sox2 mutantsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSox2 (Sox2 Mouse)
TagsFLAGExpressionMammalianMutationT258APromoterCAGAvailable sinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
AiP993-Ai195 (TIT2L-GC7s-ICF-IRES-tTA2)
Plasmid#181762Purposetargeting vectorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGCaMP7s and tTA2
ExpressionMammalianAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR: pmU6 sgChr13q34 2xMS2 pUbC MCP-mCherry-p2a-Puro
Plasmid#174119PurposeExpression of sgRNA targeting Chr13q34 (chr13: 112277011 - 112319170, hg38)DepositorInsertsgChr13q34 2xMS2 and MCP-mCherry-p2a-Puro
UseLentiviralPromotermU6 and UbCAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR: pmU6 sgChr3q29 2xMS2 pUbC MCP-mCherry-p2a-Puro
Plasmid#174118PurposeExpression of sgRNA targeting Chr3q29 (chr3: 195478317 - 195506985, hg38)DepositorInsertsgChr3q29 2xMS2 and MCP-mCherry-p2a-Puro
UseLentiviralPromotermU6 and UbCAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_PTEN(C124S)-P2A-Hygro_Barcode
Plasmid#170248PurposeBarcoded lentiviral vector to express PTEN (C124S) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_Stat3(A662C,N664C,V667L)-P2A-Hygro_Barcode
Plasmid#170254PurposeBarcoded lentiviral vector to express Stat3 (A662C, N664C, V667L) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSico_U6-PLOD2 sgRNA4
Plasmid#136459PurposeExpression of gRNA against human PLOD2DepositorInsertgRNA against human PLOD2 (PLOD2 Human, Synthetic)
UseCRISPR and LentiviralExpressionMammalianAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-FLEX-rc [ChromeQ-GFP]
Plasmid#153540PurposeAAV-mediated expression of ChromeQ-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner.DepositorInsertChromeQ-GFP
UseAAVTagsGFPExpressionMammalianPromoterSynAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWPXL-p150-PIP1Δ
Plasmid#160993PurposeExpress mouse p150 protein with PIP1 deletion mutationDepositorInsertCHAF1A (Chaf1a Mouse)
TagsGFPExpressionMammalianMutationPIP1 (PCNA-interacting protein) motif deletionAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
908E
Plasmid#160238PurposeTtgO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attBDepositorInsertsTtg Operator (2x)
hsp70 minimal promoter
p-element promoter
Firefly luciferase
Renilla luciferase
Ubiquitin promoter
TagsT2A GFPExpressionInsectAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
908G
Plasmid#160239PurposePipO(3x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attBDepositorInsertsPip Operator (3x)
hsp70 minimal promoter
p-element promoter
Firefly luciferase
Renilla luciferase
Ubiquitin promoter
TagsT2A GFPExpressionInsectAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only