We narrowed to 15,824 results for: LEA

1. pHL718

   Plasmid

   #62826

   Purpose

   Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::ipex::mCherry::T1 terminator + ColE1

   Depositor

   Han Lim

   Article

   Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

   Inserts

   gfp
   mCherry
   

   Use

   Synthetic Biology

   Tags

   ipex leader and ompC leader

   Expression

   Bacterial

   Mutation

   Promoter

   pLlacO-1 and pLtetO-1

   Available since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pBT309a_pBS-U-attPx3-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5

   Plasmid

   #52542

   Purpose

   Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

   Depositor

   Liqun Luo

   Article

   Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

   Inserts

   PhiC31o attPx3 (70 bp each)
   Neo
   PhiC31o integrase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. myc-actin-NES-1

   Plasmid

   #60618

   Purpose

   Encodes MYC tagged beta-actin with a mutation in the NES

   Depositor

   Primal de Lanerolle

   Article

   Hofmann et al J Cell Biol. 2009 Jul 27;186(2):193-200.

   Insert

   Beta-actin (ACTB Human)
   

   Use

   Tags

   MYC

   Expression

   Bacterial and Mammalian

   Mutation

   I178A, L180A

   Promoter

   Available since

   Nov. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pGEX-4T3-ZO-2-1st-PDZ

   Plasmid

   #27424

   Depositor

   Marius Sudol

   Article

   Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

   Insert

   Zonula Occludens 2 (TJP2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 31, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pGEX4T-1_OCT4_T234A_S235A

   Plasmid

   #40634

   Depositor

   James Thomson

   Article

   Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

   Insert

   POU5F1 (POU5F1 Human)
   

   Use

   Tags

   GST, TEV cleavage, and Thrombin cleavage

   Expression

   Mammalian

   Mutation

   T234A_S235A

   Promoter

   tac

   Available since

   July 26, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pGEX4T-1_OCT4_T234E_S235E

   Plasmid

   #40635

   Depositor

   James Thomson

   Article

   Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

   Insert

   POU5F1 (POU5F1 Human)
   

   Use

   Tags

   GST, TEV Cleavage, and Thrombin Cleavage

   Expression

   Bacterial

   Mutation

   T234E_S235E

   Promoter

   tac

   Available since

   July 26, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pGEX4T-1_OCT4_T234E

   Plasmid

   #40636

   Depositor

   James Thomson

   Article

   Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

   Insert

   POU5F1 (POU5F1 Human)
   

   Use

   Tags

   GST, TEV Cleavage, and Thrombin Cleavage

   Expression

   Bacterial

   Mutation

   T234E

   Promoter

   tac

   Available since

   July 26, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pGEX4T-1_OCT4_T234A

   Plasmid

   #40637

   Depositor

   James Thomson

   Article

   Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

   Insert

   POU5F1 (POU5F1 Human)
   

   Use

   Tags

   GST, TEV cleavage, and Thrombin cleavage

   Expression

   Bacterial

   Mutation

   T234A

   Promoter

   tac

   Available since

   July 26, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pGEX4T-1_OCT4_S235E

   Plasmid

   #40638

   Depositor

   James Thomson

   Article

   Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

   Insert

   POU5F1 (POU5F1 Human)
   

   Use

   Tags

   GST, TEV Cleavage, and Thrombin Cleavage

   Expression

   Bacterial

   Mutation

   S235E

   Promoter

   tac

   Available since

   July 26, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pEBB-GFP-hBEN-deltaNLS3

    Plasmid

    #22158

    Depositor

    Ananda Roy

    Article

    Tussie-Luna et al Proc Natl Acad Sci U S A. 2001 Jul 3. 98

    Insert

    hBEN (GTF2IRD1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 883-889

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA4/HisMaxB-YAP2-deltaC

    Plasmid

    #21124

    Depositor

    Marius Sudol

    Article

    Tsutomu Oka & Marius et al

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    5 Carboxy-terminal amino acids -FLTWL are deleted

    Promoter

    Available since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pWay21-MKP-1CH2A

    Plasmid

    #13473

    Depositor

    Anton Bennett

    Article

    Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

    Insert

    MKP-1CH2A (Dusp1 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Contains aa 1-46

    Promoter

    Available since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pWay21-MKP-1CH2B

    Plasmid

    #13474

    Depositor

    Anton Bennett

    Article

    Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

    Insert

    MKP-1CH2B (Dusp1 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Contains aa 47-136

    Promoter

    Available since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

14. PS Intein NLS-NES

    Plasmid

    #242021

    Purpose

    Blue light-induced reconstitution of mCherry and its relocalization to the nucleus.

    Depositor

    Vladislav Verkhusha

    Article

    Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

    Insert

    PS Intein NLS-NES
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Split PS Intein

    Plasmid

    #242022

    Purpose

    Blue-light-induced reconstitution of split mCherry. A T2A peptide is inserted at the natural split site of gp41-1.

    Depositor

    Vladislav Verkhusha

    Article

    Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

    Insert

    Split PS Intein
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PS Intein/C1A Bid

    Plasmid

    #242025

    Purpose

    PS Intein Bid construct containing the C1A mutation in gp41-1, which abolishes its autocatalytic splicing activity.

    Depositor

    Vladislav Verkhusha

    Article

    Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

    Insert

    PS Intein/C1A Bid
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. His-Thrombin-Munc13-1 C1-C2B-MUN-C2C R1598E F1658E (delta1408-1452 delta1533-1551)

    Plasmid

    #243822

    Purpose

    Munc13-1 protein purification

    Depositor

    Jose Rizo-Rey

    Article

    Quade et al Elife. 2019 Feb 28;8:e42806. doi: 10.755

    Insert

    Munc13-1 (Unc13a Rat)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    delta1408-1452 delta1533-1551 R1598E F1658E

    Promoter

    Available since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNS1D-sgRNA9-SpR

    Plasmid

    #191092

    Purpose

    aTc inducible gRNA expression plasmid with BbsI sites for Golden Gate insertion of crRNA oligos and homology arms for genome integration at NS1D in Synechococcus sp. PCC 7002

    Depositor

    Anne Ruffing

    Article

    Dallo et al ACS Synth Biol. 2023 Apr 21;12(4):1175-1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tet

    Available since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LentiCRISPR-B_2xsgRNAs-RMRP (pAVA3583)

    Plasmid

    #239260

    Purpose

    Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting RMRP

    Depositor

    Andrei Alexandrov

    Article

    Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

    Insert

    U6-driven sgRNA1 and 7SK-driven sgRNA2 targeting RMRP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LentiCRISPR-B_2xsgRNAs-RMRP (pAVA3584)

    Plasmid

    #239261

    Purpose

    Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting RMRP

    Depositor

    Andrei Alexandrov

    Article

    Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

    Insert

    U6-driven sgRNA1 and 7SK-driven sgRNA2 targeting RMRP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge