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pT7-L3T1L

Plasmid

#33288

Depositor

Terence Dermody

Article

Kobayashi et al Cell Host Microbe. 2007 Apr 19;1(2):147-

Insert

L3 (MoV1sL3gp1 Orthoreovirus T1L)

Expression

Mammalian

Promoter

T7

Available Since

Dec. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-HCA_sensor

Plasmid

#127182

Purpose

Eukaryotic expression of the fusion protein used for sulfa-drugs sensing

Depositor

Kai Johnsson

Article

Scarabelli et al ACS Sens. 2017 Aug 25;2(8):1191-1197. do

Insert

SNAP-YPeT-Pro15-GGS2-Pro15-CFP-HCA

Tags

HisTag and StrepTag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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mEos2-Beta-Catenin-20

Plasmid

#57350

Purpose

Localization: Tight Junctions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Beta-Catenin (Ctnnb1 Mouse)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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Dendra2-Keratin-17

Plasmid

#57726

Purpose

Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Keratin (KRT18 Human)

Tags

Dendra2

Expression

Mammalian

Mutation

V282L

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits
miniSOG-Lifeact-7

Plasmid

#54823

Purpose

Localization: Actin, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Lifeact-miniSOG

Tags

Lifeact

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits
Clover-Lysosomes-20

Plasmid

#56528

Purpose

Localization: Lysosome Membrane, Excitation: 505, Emission: 515

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

LAMP1

Tags

Clover

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mPapaya1-PDHA1-N-10

Plasmid

#56356

Purpose

Localization: Mitochondria, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

PDHA1 (PDHA1 Human)

Tags

mPapaya1

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pT7-M3T1L

Plasmid

#33291

Depositor

Terence Dermody

Article

Kobayashi et al Cell Host Microbe. 2007 Apr 19;1(2):147-

Insert

M3 (MoV1sM3gp1 Orthoreovirus T1L)

Expression

Mammalian

Promoter

T7

Available Since

Dec. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-S-Luc_mito

Plasmid

#113927

Purpose

Plasmid encoding the mitochondrial localization of the chimeric SNAP-NanoLuc fusion S-Luc in mammalian cells for BRET

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

S-Luc_mito

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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mCitrine-VE-Cadherin-N-10

Plasmid

#56319

Purpose

Localization: Tight Junctions, Excitation: 516, Emission: 529

Depositor

Michael Davidson

Article

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

VE-Cadherin (CDH5 Human)

Tags

mCitrine

Expression

Mammalian

Mutation

I503T in VE-Cadherin

Promoter

CMV

Available Since

Dec. 19, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pCA528-AMOT(1-300)-sortase

Plasmid

#166706

Purpose

expresses AMOT(1-300) with C-terminal sortase tag in bacterial cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

AMOT aa 1-300

Tags

His-SUMO and Sortase tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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mEos2-Calnexin-N-14

Plasmid

#57352

Purpose

Localization: Endoplasmic Reticulum, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Calnexin (CANX Human)

Tags

mEos2

Expression

Mammalian

Mutation

I474N in Calnexin

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
VYAIFIFQL_epitope

Plasmid

#165018

Purpose

Expression of CMV US20 (29-37) fused to IL-2 signal sequence

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

CMV US20 (29-37)

Use

Lentiviral

Tags

human IL2 signal sequence at N-term

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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mEos2-H1-10

Plasmid

#57383

Purpose

Localization: Nucleus/Histones, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

H1 (H1f0 Mouse)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pET51b(+)_S-Luc_CLIP

Plasmid

#113923

Purpose

Plasmid encoding the bacterial expression of the chimeric SNAP-NanoLuc fusion dubbed S-Luc c-terminally linked to CLIP-tag for BRET

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

S-Luc_CLIP

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.shNkx2-1

Plasmid

#32400

Depositor

Tyler Jacks, Monte Winslow

Article

Winslow et al Nature. 2011 May 5. 473(7345):101-4.

Insert

shRNA targetting Nkx2-1 (Nkx2-1 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
mEmerald-C-Src-7

Plasmid

#54052

Purpose

Localization: Membrane/Vesicles, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

Insert

C-Src

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pEBTet-p52-HDM2_sensor

Plasmid

#127184

Purpose

Eukaryotic expression of the fusion protein used for drug diruption the p53-HDM2 interaction

Depositor

Kai Johnsson

Article

Scarabelli et al ACS Sens. 2017 Aug 25;2(8):1191-1197. do

Insert

p53-YPeT-Pro15-GGS2-Pro15-CFP-HDM2

Tags

HisTag and StrepTag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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pJin-290

Plasmid

#125718

Purpose

ABA-inducible split T7 gRNA for GFP vector using RNAPN d5-19 with CGG RNAP-C

Depositor

Bryan Dickinson

Article

Pu et al ACS Chem Biol. 2018 Feb 16;13(2):431-437

Insert

PCGG-gRNA GFP-off expression, FKBP-linker-T7 RNAPC, T7 RNAPN (d5-19)-linker-FRB

Expression

Mammalian

Available Since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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mTFP1-VSVG-16

Plasmid

#55517

Purpose

Localization: Golgi/ER Transport, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

VSVG

Tags

mTFP1

Expression

Mammalian

Mutation

deltaK VSVG ts045

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only