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pENTR1A 2xHA HCAR3

Plasmid

#226807

Purpose

Gateway entry vector for 2xHA HCAR3

Depositor

Ramiro Iglesias-Bartolome

Publication

Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

Insert

HCAR3 (HCAR3 Human)

Use

Gateway Cloning

Tags

2xHA tag followed by a GDPPVAT linker

Mutation

Sequence optimized from NP_006009.2

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pME-lox-3xNLS-mCherry-V5-SV40pA-3xstop-lox (JDW 1232)

Plasmid

#224522

Purpose

A Gateway compatible middle entry clone containing loxP flanked 3xNLS-mCherry-V5-3xSTOP cassette

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

lox-3xNLS-mCherry-V5-SV40pA-3xstop-lox

Use

Cre/Lox

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCA16-Ubc-NLS-HA-dSaCas9-NLS-[10XGCN4]-P2A-BSD

Plasmid

#199447

Purpose

Expession of dSaCas9-10XGCN4 that recruits scFV-tdTomato (pCA15) for amplifying fluorescent signal

Depositor

Baohui Chen

Publication

Xu et al J Virol. 2022 Dec 21;96(24):e0092022. do

Insert

dSaCas9-10xGCN4

Use

Lentiviral

Expression

Mammalian

Promoter

human ubiquitin C (UBC)

Available since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pmCherry2-CD86-mEYFP(Q69K)-mEGFP

Plasmid

#190751

Purpose

Expression of triple tagged CD86 with mCherry2, mEYFP(Q69K), and mEGFP in mammalian cells.

Depositor

Dave Piston

Publication

Foust et al Biophys J. 2025 Oct 21;124(20):3354-3365

Insert

CD86 (CD86 Human)

Tags

mCherry2, mEGFP, and mEYFP(Q69K)

Expression

Mammalian

Promoter

CMV

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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Akaluc VLP

Plasmid

#182212

Purpose

YIp for expressing MPyV deltaVP1 (GAL1 promoter) and Akaluc tagged with VP2C (GAL10 promoter).

Depositor

Claudia Vickers

Inserts

VP2C-Akaluc
Murine polyomavirus deltaVP1 (NLS deletion mutant)

Use

Synthetic Biology; Tem imaging

Expression

Yeast

Promoter

GAL1 and GAL10

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α1.1-FLEX-rc [ChromeT-GFP]

Plasmid

#153541

Purpose

AAV-mediated expression of ChromeT-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner.

Depositor

Edward Boyden

Publication

Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

Insert

ChromeT-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

EF1α1.1

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-FLEX-rc [ChromeT-GFP]

Plasmid

#153539

Purpose

AAV-mediated expression of ChromeT-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner.

Depositor

Edward Boyden

Publication

Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

Insert

ChromeT-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

Syn

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-B8-VN

Plasmid

#135511

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-B*08:01

Depositor

Erik Procko

Publication

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-B*08:01 (HLA-B Human)

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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FN(9-10, RGE)-I27-CC

Plasmid

#85396

Purpose

MTFM mechanosensor for integrin. Fibronectin domains 9&10 with RGE mutation fused to titan I27 domain with two cysteines for immobilization, site for p-azidophenylalanine incorporation & Cy3 labeling

Depositor

Khalid Salaita

Publication

Galior et al Nano Lett. 2016 Jan 13;16(1):341-8. doi:

Insert

Fibronectin (FN) domains 9&10 containing RGE mutation fused to titin immunoglobulin domain (I27), containing a TAG (amber) codon

Tags

6x His

Expression

Bacterial

Mutation

GRGDS motif mutated to GRGES

Promoter

T7

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pCS3-MT-BX-6MYC p21 1-89

Plasmid

#78784

Purpose

To overexpress p21 1-89 in Mammalian Cells

Depositor

Jaewhan Song

Publication

Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

Insert

p21 (CDKN1A Human)

Tags

6xMYC

Expression

Mammalian

Mutation

Jan-89

Promoter

CMV IE94

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only
p-miR-SC

Plasmid

#65055

Purpose

Expression of miR148a with scrambled seed in mammalian cells

Depositor

Cristina Fillat

Publication

Bofill-De Ros et al Mol Ther. 2014 Sep;22(9):1665-77. doi: 1

Insert

hsa-miR-148a (mutated) (MIR148A Human)

Use

Retroviral

Expression

Mammalian

Promoter

SV40

Available since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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pHR-SIN-PTEN-del(1-25)

Plasmid

#30390

Depositor

Todd Waldman

Publication

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

PTEN-del(1-25) (PTEN Human)

Expression

Mammalian

Mutation

del(1-25)

Available since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
pHR-SIN-PTEN-del(1-15)

Plasmid

#30389

Depositor

Todd Waldman

Publication

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

PTEN-del(1-15) (PTEN Human)

Expression

Mammalian

Mutation

del(1-15)

Available since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
pHR-SIN-PTEN-L25A

Plasmid

#30388

Depositor

Todd Waldman

Publication

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

PTEN-L25A (PTEN Human)

Expression

Mammalian

Mutation

L25A

Available since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
pHR-SIN-PTEN-D24N

Plasmid

#30387

Depositor

Todd Waldman

Publication

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

PTEN-D24N (PTEN Human)

Expression

Mammalian

Mutation

D24N

Available since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pHR-SIN-PTEN-L23F

Plasmid

#30386

Depositor

Todd Waldman

Publication

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

PTEN-L23F (PTEN Human)

Expression

Mammalian

Mutation

L23F

Available since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
pHR-SIN-PTEN-F21A

Plasmid

#30385

Depositor

Todd Waldman

Publication

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

PTEN-F21A (PTEN Human)

Expression

Mammalian

Mutation

F21A

Available since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
pHR-SIN-PTEN-R14A

Plasmid

#30384

Depositor

Todd Waldman

Publication

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

PTEN-R14A (PTEN Human)

Expression

Mammalian

Mutation

R14A

Available since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
pHR-SIN-PTEN-R11A

Plasmid

#30383

Depositor

Todd Waldman

Publication

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

PTEN-R11A (PTEN Human)

Expression

Mammalian

Mutation

R11A

Available since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
pHR-SIN-PTEN-K6A

Plasmid

#30382

Depositor

Todd Waldman

Publication

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

PTEN-K6A (PTEN Human)

Expression

Mammalian

Mutation

K6A

Available since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only